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Recombinant Human His6-USP1/His6-UAF1 Complex Protein, CF

Catalog #: E-568
4 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
E-568-050
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (4)
FAQs
Reviews (1)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human His6-USP1/His6-UAF1 Complex Protein, CF Summary

Product Specifications

Activity
Recombinant Human USP1/UAF1 complex is a Ubiquitin-specific deconjugating enzyme heterodimer. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human USP1/UAF1 complex concentration of 0.01-0.5 μM.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP1/UAF1 Complex protein
Met1 - Leu785 (Gly670Ala, Gly671Ala) with a C-terminal 6-His tag (USP1), Met1 - Thr677 with a C-terminal 6-His tag (UAF1)
Accession #
O94782.1 (USP1), Q8TAF3.1 (UAF1)
Predicted Molecular Mass
89 kDa (USP1), 77 kDa (UAF1)

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E-568

Product Datasheet
COA
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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-568

Formulation Supplied as a 0.2 μm filtered solution in HEPES, NaCl, TCEP and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: USP1/UAF1 Complex

USP1 (Ubiquitin-Specific-processing Protease 1, also known as Ubiquitin carboxyl-terminal hydrolase 1) is a deubiquitinating enzyme of the C19 peptidase family and functions as a negative regulator of the Fanconi Anemia pathway. Reported substrates of USP1 include monoubiquitinated FANCD2 and monoubiquitinated PCNA. USP1 plays important roles in DNA damage responses and cancer-related processes, and inhibiting the function of this deubiquitinase sensitizes some cancer cells to chemotherapy. Alone, USP1 is nearly completely inactive and requires a protein binding partner, UAF1 (USP1-Associated Factor 1, also known as WD Repeat-containing protein 48 or WDR48) to stimulate its deubiquitinase activity. In addition to the naturally occurring substates listed above, the USP1/UAF1 complex has been demonstrated to cleave K6, K33, and K63-linked polyubiquitin chains. This product is manufactured by combining His6-USP1 and His6-UAF1 at a 1:1 mass ratio.

References
  1. Cohn, M.A., et al. (2009) J. Biol. Chem. 284: 5343.
  2. Huang, T.T., et al. (2006) Nat. Cell Biol. 8: 339.
  3. Joo, H-Y., et al. (2011) Biol. Chem. 286: 7190.
  4. Moretti, J., et al. (2012) J. Biol. Chem. 287: 29429.
  5. Williams, S.A, et al. (2011) Cell 146: 918.
Long Name
Ubiquitin Specific Protease 1/USP1-associated Factor 1 Complex
Entrez Gene IDs
7398 (Human)
Alternate Names
USP1/UAF1 Complex

Citations for Recombinant Human His6-USP1/His6-UAF1 Complex Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. CRISPR/Cas9-based genome-wide screening for deubiquitinase subfamily identifies USP1 regulating MAST1-driven cisplatin-resistance in cancer cells
    Authors: A Tyagi, K Kaushal, AP Chandrasek, N Sarodaya, S Das, CH Park, SH Hong, KS Kim, S Ramakrishn
    Theranostics, 2022-08-08;12(13):5949-5970.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  2. Identification of deubiquitinase targets of isothiocyanates using SILAC-assisted quantitative mass spectrometry
    Authors: AP Lawson, DW Bak, DA Shannon, MJC Long, T Vijaykumar, R Yu, FE Oualid, E Weerapana, L Hedstrom
    Oncotarget, 2017-04-20;8(31):51296-51316.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  3. Mechanism of Ubiquitination and Deubiquitination in the Fanconi Anemia Pathway
    Authors: Sylvie van Twest
    Mol. Cell, 2016-12-13;0(0):.
    Applications: Bioassay
  4. USP7 is a SUMO deubiquitinase essential for DNA replication
    Authors: E Lecona, S Rodriguez-, J Specks, AJ Lopez-Cont, I Ruppen, M Murga, J Muñoz, J Mendez, O Fernandez-
    Nat Struct Mol Biol, 2016-03-07;23(4):270-7.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay

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Recombinant Human His6-USP1/His6-UAF1 Complex Protein, CF
By Anonymous on 04/29/2019
Application: Enzymatic activity in vitro
Enzymatic activity in vitro Recombinant Human His6-USP1/His6-UAF1 Complex Protein, CF E-568