Home / Ubiquitin-activating Enzyme/UBE1 / Recombinant Human His6-Ubiquitin E1 Enzyme (UBE1), CF

Recombinant Human His6-Ubiquitin E1 Enzyme (UBE1), CF

Catalog #: E-304
64 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
E-304-050
R&D Systems Recombinant Proteins and Enzymes
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Citations (64)
FAQs
Reviews (2)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human His6-Ubiquitin E1 Enzyme (UBE1), CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Recombinant Human His6-Ubiquitin Activating Enzyme (UBE1) is a member of the Ubiquitin-activating (E1) enzyme family that is required for the first step of the enzymatic cascade that subsequently utilizes a Ubiquitin-conjugating (E2) enzyme and a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-Ubiquitin Activating Enzyme (UBE1) concentration of 50-200 nM.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Ubiquitin-activating Enzyme/UBE1 protein
Contains an N-terminal Met-Ser-Tyr-Tyr and 6-His tag
Accession #
P22314.3
Predicted Molecular Mass
121 kDa

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E-304

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-304

Formulation Supplied as a solution in HEPES, NaCl and TCEP.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ubiquitin-activating Enzyme/UBE1

Ubiquitin-activating Enzyme (UBE1), also known as Ubiquitin-like Modifier Activating Enzyme 1 (UBA1), is a 1058 amino acid (aa) canonical member of the Ubiquitin-activating (E1) enzyme family of proteins with a predicted molecular weight of 118 kDa.  It is ubiquitously expressed and highly conserved; mouse and rat UBE1 share 95% and 96% aa sequence identity with the human UBE1 protein, respectively. UBE1 is found in the cytoplasm and nucleus, and contains a conserved active-site cysteine residue and ATP-binding site common to E1 enzymes (1-3). UBE1 is responsible for the first step in Ubiquitin-protein isopeptide bond formation (4,5). Ubiquitin is activated by UBE1 and thereafter linked to the side chain of a cysteine residue in UBE1, Cys632 in humans, yielding a Ubiquitin-UBE1 conjugate via a thioester bond (5-8). The activated Ubiquitin is then transferred to a lysine residue on the target protein via the Ubiquitin-conjugating  – Ubiquitin ligase enzyme cascade. UBE1 is required for cell cycle progression and has been linked to cellular responses to DNA damage such as nucleotide excision repair (3,9,10). Mutations in UBE1 are associated with X-linked lethal infantile spinal muscular atrophy (11).  UBE1 is a critical component for the initiation of in vitro ubiquitin conjugation reactions. 

 

References
  1. Handley, P.M. et al. (1991) Proc. Natl. Acad. Sci. USA 88:258.
  2. Nagai, Y. et al. (1995) J. Cell Sci. 108:2145.
  3. Stephen, A.G. et al. (1996) J. Biol. Chem. 271:15608.
  4. Hershko, A. et al. (1983) J. Biol. Chem. 258:8206.
  5. Schulman, B.A. & J.W. Harper (2009) Nat. Rev. Mol. Cell Biol. 10:319.
  6. Haas, A.L. et al. (1982) J. Biol. Chem. 257:2543.
  7. Haas, A.L. & I.A. Rose (1982) J. Biol. Chem. 257:10329.
  8. Pickart, C.M. et al. (1994) J. Biol. Chem. 269:7115.
  9. Nouspikel, T. & P.C. Hanawalt (2006) Proc. Natl. Acad. Sci. USA 103:16188.
  10. Moundry, P. et al. (2012) Cell Cycle 11:1573.
  11. Ramser, J. et al. (2008) Am. J. Hum. Genet. 82:188.
Entrez Gene IDs
7317 (Human); 22201 (Mouse); 314432 (Rat); 100009225 (Rabbit); 853670 (Yeast)
Alternate Names
A1S9T and BN75 temperature sensitivity complementing; A1S9T; A1ST; AMCX1; GXP1; MGC4781; POC20 centriolar protein homolog; POC20; Protein A1S9; SMAX2; UBA1; UBA1, ubiquitin-activating enzyme E1 homolog A; UBA1A; UBE1; UBE1A1S9; UBE1X; ubiquitin-activating enzyme E1 (A1S9T and BN75 temperature sensitivitycomplementing); Ubiquitin-activating enzyme E1; Ubiquitinactivating Enzyme; Ubiquitin-activating Enzyme; ubiquitin-like modifier activating enzyme 1; ubiquitin-like modifier-activating enzyme 1

Citations for Recombinant Human His6-Ubiquitin E1 Enzyme (UBE1), CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

64 Citations: Showing 1 - 10
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  1. Lipid-mediated intracellular delivery of recombinant bioPROTACs for the rapid degradation of undruggable proteins
    Authors: Chan, A;Haley, RM;Najar, MA;Gonzalez-Martinez, D;Bugaj, LJ;Burslem, GM;Mitchell, MJ;Tsourkas, A;
    Nature communications
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Structure of the p53 degradation complex from HPV16
    Authors: Wang, JCK;Baddock, HT;Mafi, A;Foe, IT;Bratkowski, M;Lin, TY;Jensvold, ZD;Preciado López, M;Stokoe, D;Eaton, D;Hao, Q;Nile, AH;
    Nature communications
    Species: Bacteria - Escherichia coli
    Sample Types: Whole Cells
    Applications: Bioassay
  3. DELTEX E3 ligases ubiquitylate ADP-ribosyl modification on nucleic acids
    Authors: Zhu, K;Suskiewicz, MJ;Chatrin, C;Strømland, Ø;Dorsey, BW;Aucagne, V;Ahel, D;Ahel, I;
    Nucleic acids research
    Species: N/A
    Sample Types: Recombinant Proteins
    Applications: Bioassay
  4. Structure of the DDB1-AMBRA1 E3 ligase receptor complex linked to cell cycle regulation
    Authors: Liu, M;Wang, Y;Teng, F;Mai, X;Wang, X;Su, MY;Stjepanovic, G;
    Nature communications
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay
  5. Selective Smurf1 E3 ligase inhibitors that prevent transthiolation
    Authors: Chuong, P;Statsyuk, A;
    bioRxiv : the preprint server for biology
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay
  6. A CK2 and SUMO-dependent, PML NB-involved regulatory mechanism controlling BLM ubiquitination and G-quadruplex resolution
    Authors: Liu, S;Atkinson, E;Paulucci-Holthauzen, A;Wang, B;
    Nature communications
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay
  7. Coupled deglycosylation-ubiquitination cascade in regulating PD-1 degradation by MDM2
    Authors: Wu, Z;Cao, Z;Yao, H;Yan, X;Xu, W;Zhang, M;Jiao, Z;Zhang, Z;Chen, J;Liu, Y;Zhang, M;Wang, D;
    Cell reports
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  8. Monitoring ?-synuclein ubiquitination dynamics reveals key endosomal effectors mediating its trafficking and degradation
    Authors: Zenko, D;Marsh, J;Castle, AR;Lewin, R;Fischer, R;Tofaris, GK;
    Science advances
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  9. Parkin Precipitates on Mitochondria via Aggregation and Autoubiquitination
    Authors: Ardah, MT;Radwan, N;Khan, E;Kitada, T;Haque, ME;
    International journal of molecular sciences
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay
  10. HAPSTR1 localizes HUWE1 to the nucleus to limit stress signaling pathways
    Authors: Monda, JK;Ge, X;Hunkeler, M;Donovan, KA;Ma, MW;Jin, CY;Leonard, M;Fischer, ES;Bennett, EJ;
    Cell reports
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay
  11. Calpain activity is negatively regulated by a KCTD7-Cullin-3 complex via non-degradative ubiquitination
    Authors: J Sharma, S Mulherkar, UI Chen, Y Xiong, L Bajaj, BK Cho, YA Goo, HE Leung, KF Tolias, M Sardiello
    Cell Discovery, 2023-03-24;9(1):32.
    Species: Human
    Sample Types: Whole Cells
    Applications: Ubiquitination
  12. Calpain activity is negatively regulated by a KCTD7-Cullin-3 complex via non-degradative ubiquitination
    Authors: J Sharma, S Mulherkar, UI Chen, Y Xiong, L Bajaj, BK Cho, YA Goo, HE Leung, KF Tolias, M Sardiello
    Cell Discovery, 2023;9(1):32.
    Species: Human
    Sample Types: Whole Cells
    Applications: Ubiquitination
  13. Cisplatin-induced cell death increases the degradation of the MRE11-RAD50-NBS1 complex through the autophagy/lysosomal pathway
    Authors: A Belmonte-F, J Herrero-Ru, M Galindo-Mo, MC Limón-Mort, M Mora-Santo, C Sáez, MÁ Japón, M Tortolero, F Romero
    Cell Death and Differentiation, 2022-12-08;0(0):.
    Species: Human
    Sample Types: Recombinant Proteins
    Applications: Bioassay
  14. DELTEX E3 ligases ubiquitylate ADP-ribosyl modification on protein substrates
    Authors: K Zhu, MJ Suskiewicz, A Hloušek-Ka, H Meudal, A Miko?, V Aucagne, D Ahel, I Ahel
    Science Advances, 2022-10-05;8(40):eadd4253.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  15. Induction of zinc finger protein RNF6 auto-ubiquitination for the treatment of myeloma and chronic myeloid leukemia
    Authors: H Zhuang, Y Ren, C Mao, Y Zhong, Z Zhang, B Cao, Y Zhang, J Huang, G Xu, Z Huang, Y Xu, X Mao
    The Journal of Biological Chemistry, 2022-08-01;298(9):102314.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay
  16. Membrane Targeting and GTPase Activity of Rab7 Are Required for Its Ubiquitination by RNF167
    Authors: K Ghilarducc, VC Cabana, A Harake, L Cappadocia, MP Lussier
    International Journal of Molecular Sciences, 2022-07-16;23(14):.
    Species: Human
    Sample Types: Recombinant Proteins
    Applications: Bioassay
  17. Regulated interaction of ID2 with the anaphase-promoting complex links progression through mitosis with reactivation of cell-type-specific transcription
    Authors: SB Lee, L Garofano, A Ko, F D'Angelo, B Frangaj, D Sommer, Q Gan, K Kim, T Cardozo, A Iavarone, A Lasorella
    Nature Communications, 2022-04-19;13(1):2089.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  18. Cisplatin-induced cell death increases the degradation of the MRE11-RAD50-NBS1 complex through the autophagy/lysosomal pathway
    Authors: A Belmonte-F, J Herrero-Ru, M Galindo-Mo, MC Limón-Mort, M Mora-Santo, C Sáez, MÁ Japón, M Tortolero, F Romero
    Cell Death and Differentiation, 2022;0(0):.
    Species: Human
    Sample Types: Recombinant Proteins
    Applications: Bioassay
  19. Identification of disease-linked hyperactivating mutations in UBE3A through large-scale functional variant analysis
    Authors: KP Weston, X Gao, J Zhao, KS Kim, SE Maloney, J Gotoff, S Parikh, YC Leu, KP Wu, M Shinawi, JP Steimel, JS Harrison, JJ Yi
    Nature Communications, 2021-11-23;12(1):6809.
    Species: Human
    Sample Types: Recombinant Proteins
    Applications: Bioassay
  20. G3BP1 inhibits Cul3SPOP to amplify AR signaling and promote prostate cancer
    Authors: C Mukhopadhy, C Yang, L Xu, D Liu, Y Wang, D Huang, LD Deonarine, J Cyrta, E Davicioni, A Sboner, BD Robinson, AM Chinnaiyan, MA Rubin, CE Barbieri, P Zhou
    Nature Communications, 2021-11-18;12(1):6662.
    Species: Human
    Sample Types: Recombinant Proteins
    Applications: Bioassay
  21. RNF19A-mediated ubiquitination of BARD1 prevents BRCA1/BARD1-dependent homologous recombination
    Authors: Q Zhu, J Huang, H Huang, H Li, P Yi, JA Kloeber, J Yuan, Y Chen, M Deng, K Luo, M Gao, G Guo, X Tu, P Yin, Y Zhang, J Su, J Chen, Z Lou
    Nature Communications, 2021-11-17;12(1):6653.
    Species: Human
    Sample Types: Recombinant Proteins
    Applications: Bioassay
  22. A modular toolbox to generate complex polymeric ubiquitin architectures using orthogonal sortase enzymes
    Authors: M Fottner, M Weyh, S Gaussmann, D Schwarz, M Sattler, K Lang
    Nature Communications, 2021-11-11;12(1):6515.
    Species: Human
    Sample Types: Recombinant Proteins
    Applications: Bioassay
  23. Structure of the FA core ubiquitin ligase closing the ID clamp on DNA
    Authors: S Wang, R Wang, C Peralta, A Yaseen, NP Pavletich
    Nature structural & molecular biology, 2021-03-08;28(3):300-309.
    Species: Human, N/A
    Sample Types: Protein, Whole Cells
    Applications: Ubiquitination
  24. The deubiquitinase TRABID stabilizes the K29/K48-specific E3 ubiquitin ligase HECTD1
    Authors: LD Harris, J Le Pen, N Scholz, J Mieszczane, N Vaughan, S Davis, G Berridge, BM Kessler, M Bienz, JDF Licchesi
    The Journal of Biological Chemistry, 2021-01-08;296(0):100246.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  25. A role for LSH in facilitating DNA methylation by DNMT1 through enhancing UHRF1 chromatin association
    Authors: M Han, J Li, Y Cao, Y Huang, W Li, H Zhu, Q Zhao, JJ Han, Q Wu, J Li, J Feng, J Wong
    Nucleic Acids Res, 2020-12-02;0(0):.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  26. Mutual regulation between OGT and XIAP to control colon cancer cell growth and invasion
    Authors: HG Seo, HB Kim, JY Yoon, TH Kweon, YS Park, J Kang, J Jung, S Son, EC Yi, TH Lee, WH Yang, JW Cho
    Cell Death Dis, 2020-09-29;11(9):815.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  27. Extracellular matrix stiffness determines DNA repair efficiency and cellular sensitivity to genotoxic agents
    Authors: M Deng, J Lin, S Nowsheen, T Liu, Y Zhao, PW Villalta, D Sicard, DJ Tschumperl, S Lee, J Kim, Z Lou
    Science Advances, 2020-09-11;6(37):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  28. Targeting a helix-in-groove interaction between E1 and E2 blocks ubiquitin transfer
    Authors: AM Cathcart, GH Bird, TE Wales, HD Herce, EP Harvey, ZJ Hauseman, CE Newman, U Adhikary, MS Prew, T Oo, S Lee, JR Engen, LD Walensky
    Nat. Chem. Biol., 2020-08-17;0(0):.
    Species: Human, N/A
    Sample Types: Peptide, Protein
    Applications: Bioassay
  29. L ARP7 Is a BRCA1�Ubiquitinase Substrate and Regulates Genome Stability and Tumorigenesis
    Authors: F Zhang, P Yan, H Yu, H Le, Z Li, J Chen, X Liang, S Wang, W Wei, L Liu, Y Zhang, X Ji, A Xie, W Chen, Z Han, WT Pu, S Chen, Y Chen, K Sun, B Ge, B Zhang
    Cell Rep, 2020-07-28;32(4):107974.
    Species: Human
    Sample Types: Protein
    Applications: Ubiquitination
  30. piRNA-independent function of PIWIL1 as a co-activator for anaphase promoting complex/cyclosome to drive pancreatic cancer metastasis
    Authors: F Li, P Yuan, M Rao, CH Jin, W Tang, YF Rong, YP Hu, F Zhang, T Wei, Q Yin, T Liang, L Wu, J Li, D Li, Y Liu, W Lou, S Zhao, MF Liu
    Nat. Cell Biol., 2020-03-16;0(0):.
    Species: Human
    Sample Types: Recombinat Protein
    Applications: Bioassay
  31. Competitive ubiquitination activates the tumor suppressor p53
    Authors: X Li, M Guo, L Cai, T Du, Y Liu, HF Ding, H Wang, J Zhang, X Chen, C Yan
    Cell Death Differ., 2019-12-02;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  32. The FBXW7-SHOC2-Raptor Axis Controls the Cross-Talks between the RAS-ERK and mTORC1 Signaling Pathways
    Authors: CM Xie, M Tan, XT Lin, D Wu, Y Jiang, Y Tan, H Li, Y Ma, X Xiong, Y Sun
    Cell Rep, 2019-03-12;26(11):3037-3050.e4.
    Applications: Bioassay
  33. Site-specific ubiquitylation and SUMOylation using genetic-code expansion and sortase
    Authors: M Fottner, AD Brunner, V Bittl, D Horn-Ghetk, A Jussupow, VRI Kaila, A Bremm, K Lang
    Nat. Chem. Biol., 2019-02-15;15(3):276-284.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Enzyme Assay
  34. RNF126 Quenches RNF168 Function in the DNA Damage Response
    Authors: L Zhang, Z Wang, R Shi, X Zhu, J Zhou, B Peng, X Xu
    Genomics Proteomics Bioinformatics, 2018-12-04;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  35. Thalidomide promotes degradation of SALL4, a transcription factor implicated in Duane Radial Ray syndrome
    Authors: KA Donovan, J An, RP Nowak, JC Yuan, EC Fink, BC Berry, BL Ebert, ES Fischer
    Elife, 2018-08-01;7(0):.
    Species: Human
    Sample Types: Protein
    Applications: Ubiquitination
  36. The E3 ubiquitin ligase HERC1 controls the ERK signaling pathway targeting C-RAF for degradation
    Authors: T Schneider, A Martinez-M, M Cubillos-R, R Bartrons, F Ventura, JL Rosa
    Oncotarget, 2018-07-31;9(59):31531-31548.
    Applications: Bioassay
  37. Phosphorylation of Arabidopsis SINA2 by CDKG1 affects its ubiquitin ligase activity
    Authors: Y Chen, M Fokar, M Kang, N Chen, RD Allen, Y Chen
    BMC Plant Biol., 2018-07-16;18(1):147.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  38. Inheritance of the Golgi Apparatus and Cytokinesis Are Controlled by Degradation of GBF1
    Authors: R Magliozzi, ZI Carrero, TY Low, L Yuniati, C Valdes-Que, F Kruiswijk, K van Wijk, AJR Heck, CL Jackson, D Guardavacc
    Cell Rep, 2018-06-12;23(11):3381-3391.e4.
    Applications: Bioassay
  39. Peptide inhibitors of the anaphase promoting-complex that cause sensitivity to microtubule poison
    Authors: SC Schuyler, YO Wu, HY Chen, YS Ding, CJ Lin, YT Chu, TC Chen, L Liao, WW Tsai, A Huang, LI Wang, TW Liao, JH Jhuo, V Cheng
    PLoS ONE, 2018-06-08;13(6):e0198930.
    Applications: Bioassay
  40. PCGF5 is required for neural differentiation of embryonic stem cells
    Authors: M Yao, X Zhou, J Zhou, S Gong, G Hu, J Li, K Huang, P Lai, G Shi, AP Hutchins, H Sun, H Wang, H Yao
    Nat Commun, 2018-05-15;9(1):1463.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  41. Functional crosstalk between histone H2B ubiquitylation and H2A modifications and variants
    Authors: F Wojcik, GP Dann, LY Beh, GT Debelouchi, R Hofmann, TW Muir
    Nat Commun, 2018-04-11;9(1):1394.
    Applications: Bioassay
  42. Prp19/Pso4 Is an Autoinhibited Ubiquitin Ligase Activated by Stepwise Assembly of Three Splicing Factors
    Authors: TR de Moura, S Mozaffari-, CZK Szabó, J Schmitzová, O Dybkov, C Cretu, M Kachala, D Svergun, H Urlaub, R Lührmann, V Pena
    Mol. Cell, 2018-03-15;69(6):979-992.e6.
    Species: Bacteria
    Sample Types: Cell Lysates
    Applications: Bioassay
  43. Selection and Characterization of a DNA Aptamer Specifically Targeting Human HECT Ubiquitin Ligase WWP1
    Authors: WO Tucker, AB Kinghorn, LA Fraser, YW Cheung, JA Tanner
    Int J Mol Sci, 2018-03-07;19(3):.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  44. Ub-ProT reveals global length and composition of protein ubiquitylation in cells
    Authors: H Tsuchiya, D Burana, F Ohtake, N Arai, A Kaiho, M Komada, K Tanaka, Y Saeki
    Nat Commun, 2018-02-06;9(1):524.
    Applications: Bioassay
  45. A Regulatory Module Controlling Homeostasis of a Plant Immune Kinase
    Authors: J Wang, LE Grubb, J Wang, X Liang, L Li, C Gao, M Ma, F Feng, M Li, L Li, X Zhang, F Yu, Q Xie, S Chen, C Zipfel, J Monaghan, JM Zhou
    Mol. Cell, 2018-01-18;0(0):.
    Applications: Bioassay
  46. Expression, purification, and characterization of the TRIM49 protein
    Authors: DS Guimaraes, MD Gomes
    Protein Expression and Purification, 2017-11-05;143(0):57-61.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  47. Activity-Based Probes for HECT E3 ubiquitin ligases
    Authors: R Byrne, T Mund, J Licchesi
    Chembiochem, 2017-06-28;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  48. RNF8- and Ube2S-Dependent Ubiquitin Lysine 11-Linkage Modification in Response to DNA Damage
    Authors: A Paul, B Wang
    Mol. Cell, 2017-05-18;66(4):458-472.e5.
    Applications: Bioassay
  49. Ubiquitin Modification by the E3 Ligase/ADP-Ribosyltransferase Dtx3L/Parp9
    Authors: CS Yang, K Jividen, A Spencer, N Dworak, L Ni, LT Oostdyk, M Chatterjee, B Ku?mider, B Reon, M Parlak, V Gorbunova, T Abbas, E Jeffery, NE Sherman, BM Paschal
    Mol. Cell, 2017-05-18;66(4):503-516.e5.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  50. Ubiquitinated proteins promote the association of proteasomes with the deubiquitinating enzyme Usp14 and the ubiquitin ligase Ube3c
    Authors: CL Kuo, AL Goldberg
    Proc. Natl. Acad. Sci. U.S.A., 2017-04-10;114(17):E3404-E3413.
    Species: Bacteria - E. Coli
    Sample Types: Protein
    Applications: Bioassay
  51. Internally tagged ubiquitin: a tool to identify linear polyubiquitin-modified proteins by mass spectrometry
    Authors: K Kliza, C Taumer, I Pinzuti, M Franz-Wach, S Kunzelmann, B Stieglitz, B Macek, K Husnjak
    Nat. Methods, 2017-03-20;0(0):.
    Applications: Bioassay
  52. Structure-guided mutagenesis reveals a hierarchical mechanism of Parkin activation
    Authors: MY Tang, M Vranas, AI Krahn, S Pundlik, JF Trempe, EA Fon
    Nat Commun, 2017-03-09;8(0):14697.
    Applications: Bioassay
  53. Hemi-methylated DNA regulates DNA methylation inheritance through allosteric activation of H3 ubiquitylation by UHRF1
    Elife, 2016-09-06;5(0):.
    Species: Human
    Sample Types: Protein
    Applications: Ubiquitination
  54. Role of SUMO activating enzyme in cancer stem cell maintenance and self-renewal
    Nat Commun, 2016-07-28;7(0):12326.
    Applications: Bioassay
  55. Ubiquilins Chaperone and Triage Mitochondrial Membrane Proteins for Degradation
    Mol Cell, 2016-06-23;63(1):21-33.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  56. PTP1B controls non-mitochondrial oxygen consumption by regulating RNF213 to promote tumour survival during hypoxia
    Authors: RS Banh, C Iorio, R Marcotte, Y Xu, D Cojocari, AA Rahman, J Pawling, W Zhang, A Sinha, CM Rose, M Isasa, S Zhang, R Wu, C Virtanen, T Hitomi, T Habu, SS Sidhu, A Koizumi, SE Wilkins, T Kislinger, SP Gygi, CJ Schofield, JW Dennis, BG Wouters, BG Neel
    Nat. Cell Biol, 2016-06-20;18(7):803-13.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Ubiquitination
  57. The adenovirus E4-ORF3 protein functions as a SUMO E3 ligase for TIF-1? sumoylation and poly-SUMO chain elongation
    Proc Natl Acad Sci USA, 2016-05-31;113(24):6725-30.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  58. USP7 is a SUMO deubiquitinase essential for DNA replication
    Authors: E Lecona, S Rodriguez-, J Specks, AJ Lopez-Cont, I Ruppen, M Murga, J Muñoz, J Mendez, O Fernandez-
    Nat Struct Mol Biol, 2016-03-07;23(4):270-7.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  59. FBXW7 Facilitates Nonhomologous End-Joining via K63-Linked Polyubiquitylation of XRCC4
    Authors: Q Zhang, D Karnak, M Tan, TS Lawrence, MA Morgan, Y Sun
    Mol. Cell, 2016-01-07;61(3):419-33.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  60. ASH2L regulates ubiquitylation signaling to MLL: trans-regulation of H3 K4 methylation in higher eukaryotes.
    Authors: Wu L, Lee S, Zhou B, Nguyen U, Muir T, Tan S, Dou Y
    Mol Cell, 2013-02-28;49(6):1108-20.
    Applications: Bioassay
  61. Ubiquitination of neuronal nitric-oxide synthase in the calmodulin-binding site triggers proteasomal degradation of the protein.
    Authors: Clapp, Kelly M, Peng, Hwei-Min, Jenkins, Gary J, Ford, Michael, Morishima, Yoshihir, Lau, Miranda, Osawa, Yoichi
    J Biol Chem, 2012-10-29;287(51):42601-10.
    Species: Rat
    Sample Types: Recombinant Protein
    Applications: Ubiquitination
  62. The F-box protein FBXO44 mediates BRCA1 ubiquitination and degradation.
    Authors: Lu, Yunzhe, Li, Jiezhi, Cheng, Dongmei, Parameswaran, Balaji, Zhang, Shaohua, Jiang, Zefei, Yew, P Renee, Peng, Junmin, Ye, Qinong, Hu, Yanfen
    J Biol Chem, 2012-10-18;287(49):41014-22.
    Applications: Ubiquitination
  63. The H3K4me3 histone demethylase Fbxl10 is a regulator of chemokine expression, cellular morphology, and the metabolome of fibroblasts.
    Authors: Janzer, Andreas, Stamm, Katrin, Becker, Astrid, Zimmer, Andreas, Buettner, Reinhard, Kirfel, Jutta
    J Biol Chem, 2012-07-23;287(37):30984-92.
    Applications: Bioassay
  64. p47 negatively regulates IKK activation by inducing the lysosomal degradation of polyubiquitinated NEMO.
    Authors: Shibata Y, Oyama M, Kozuka-Hata H, Han X, Tanaka Y, Gohda J, Inoue J
    Nat Commun, 2012-01-01;3(0):1061.
    Applications: Binding Assay

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Recombinant Human His6-Ubiquitin E1 Enzyme (UBE1), CF
By Patrick Chuong on 11/06/2018
Application: E1 E2 E3 Autoubiquitination Assay
Other Recombinant Human His6-Ubiquitin E1 Enzyme (UBE1), CF E-304
Recombinant Human His6-Ubiquitin E1 Enzyme (UBE1), CF
By Patrick Chuong on 09/18/2018
Application: Ubiquitination Assay with E1/E2/E3