Recombinant Human HGF (NK1), Animal-Free Protein Summary
Product Specifications
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Qk013
| Formulation | Lyophilized from acetonitrile/TFA |
| Reconstitution | Resuspend in 10mM HCl at >100 µg/ml, prepare single use aliquots, add carrier protein if desired. |
| Shipping | The product is shipped lyophilized at ambient temperture, on ice blocks or dry ice. Shipping at ambient temperture does not affect the bioactivity or stability of the protein. Upon reciept, store immediately at the conditions stated below. |
| Stability & Storage: | Store lyophilized protein between -20 and -80 °C until the date of expiry. Avoid freeze-thaw cycles. |
Scientific Data
View Larger
HGF NK1 activity is determined using the Promega serum response element luciferase reporter assay (*) in transfected HEK293T cells. Cells were treated in duplicate with a serial dilution of HGF for 6 hours. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity. EC50 = 1.5 ng/ml (72.9 pM).*Promega pGL4.33[luc2P/SRE/Hygro] #E1340
View Larger
HGF NK1 migrates as major band at 20 kDa in non-reducing conditions and 18 kDa upon reduction.Purified recombinant protein (7 µg) was resolved using 15% w/v SDS-PAGE in reduced (+DTT, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250. NB reduced samples were not boiled as the protein is sensitive to high temperatures, which causes degradation.
Reconstitution Calculator
Background: HGF
HGF, also known as scatter factor and hepatopoietin A, is a pleiotropic protein in the plasminogen subfamily of S1 peptidases. It is a multidomain molecule that includes an N-terminal PAN/APPLE-like domain, four Kringle domains, and a serine proteinase-like domain that has no detectable protease activity (1-5) Human HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive disulfide-linked HGF with a 60 kDa alpha and 30 kDa beta chain. Alternate splicing generates human HGF isoforms that lack the proteinase-like domain and different numbers of the Kringle domains. Human HGF shares 91%-94% aa sequence identity with bovine, canine, feline, mouse, and rat HGF. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET (6, 7). HGF-dependent c-MET activation is implicated in the development of many human cancers (8). HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis (9, 10). HGF induces the up-regulation of integrin alpha 2 beta 1 in epithelial cells by a selective increase in alpha 2 gene transcription (11). This integrin serves as a collagen I receptor, and its blockade disrupts epithelial cell branching tubulogenesis (11, 12). HGF can also alter epithelium morphology by the induction of nectin-1 alpha ectodomain shedding, an adhesion protein component of adherens junctions (13). In the thyroid, HGF induces the proliferation, motility, and loss of differentiation markers of thyrocytes and inhibits TSH-stimulated iodine uptake (14). HGF promotes the motility of cardiac stem cells in damaged myocardium (15).
- Karihaloo, A. et al. (2005) Nephron Exp. Nephrol. 100:e40.
- Hammond, D.E. et al. (2004) Curr. Top. Microbiol. Immunol. 286:21.
- Rosario, M. and Birchmeier, W. (2004) Dev. Cell 7:3.
- Lesk, A.M. and Fordham, W.D. (1996) J. Mol. Biol. 258:501.
- Nakamura, T. et al. (1989) Nature 342:440.
- Mizuno, K., et al. (1994) J. Biol. Chem. 269:1131.
- Gheradi, E. et al. (2003) Proc. Natl. Acad. Sci. 100:12039.
- Corso, S. et al. (2005) Trends Mol. Med. 11:284.
- Maeshima, A. et al. (2000) Kid. Int. 58:1511.
- Montesano, R. et al. (1991) Cell 67:901.
- Chiu, S-J. et al. (2002) J. Biomed. Sci. 9:261.
- Saelman, E.U.M. et al. (1995) J. Cell Sci. 108:3531.
- 13. Tanaka, Y. et al. (2002) Biochem. Biophys. Res. Commun. 299:472.
- 14. Mineo, R. et al. (1994) Endocrinology 145:4355.
- 15. Urbanek, K. et al. (2005) Circ. Res. 97:663.
Product Specific Notices
The above product was manufactured, tested and released by R&D System's contract manufacturer, Qkine Ltd, at 1 Murdoch House, Cambridge, UK, CB5 8HW. The product is for research use only and not for the diagnostic or theraputic use.FAQs
No product specific FAQs exist for this product, however you may
View all Proteins and Enzyme FAQsReviews for Recombinant Human HGF (NK1), Animal-Free Protein
There are currently no reviews for this product. Be the first to review Recombinant Human HGF (NK1), Animal-Free Protein and earn rewards!
Have you used Recombinant Human HGF (NK1), Animal-Free Protein?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
