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Recombinant Human HA-Ubiquitin Vinyl Sulfone Protein, CF

Catalog #: U-212
22 Citations 1 Review Datasheet / COA / SDS
Soon to be discontinued
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U-212-025
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Citations (22)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human HA-Ubiquitin Vinyl Sulfone Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Add Recombinant Human HA-Ubiquitin-Vinyl Sulfone to in vitro assays to inhibit deubiquitinating enzymes. The HA-tag allows for detection and purification of deubiquitinating enzymes activity. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human HA-Ubiquitin-Vinyl Sulfone concentration of 1-5 μM.
Source
E. coli-derived human Ubiquitin protein
Met1 - Gly76 with a N‑terminal HA (YPYDVPDYA) tag and a C‑terminal Vinyl Sulfone
Accession #
P0CG47.1
Predicted Molecular Mass
9.9 kDa

Product Datasheets

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U-212

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

U-212

Formulation Supplied as a solution in MES.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ubiquitin

Ubiquitin is a 76 amino acid (aa) protein that is ubiquitously expressed in all eukaryotic organisms. Ubiquitin is highly conserved with 96% aa sequence identity shared between human and yeast Ubiquitin, and 100% aa sequence identity shared between human and mouse Ubiquitin (1). In mammals, four Ubiquitin genes encode for two Ubiquitin-ribosomal fusion proteins and two poly-Ubiquitin proteins. Cleavage of the Ubiquitin precursors by deubiquitinating enzymes gives rise to identical Ubiquitin monomers each with a predicted molecular weight of 8.6 kDa. Conjugation of Ubiquitin to target proteins involves the formation of an isopeptide bond between the C-terminal glycine residue of Ubiquitin and a lysine residue in the target protein. This process of conjugation, referred to as ubiquitination or ubiquitylation, is a multi-step process that requires three enzymes: a Ubiquitin-activating (E1) enzyme, a Ubiquitin-conjugating (E2) enzyme, and a Ubiquitin ligase (E3). Ubiquitination is classically recognized as a mechanism to target proteins for degradation and as a result, Ubiquitin was originally named ATP-dependent Proteolysis Factor 1 (APF-1) (2,3). In addition to protein degradation, ubiquitination has been shown to mediate a variety of biological processes such as signal transduction, endocytosis, and post-endocytic sorting (4-7).

This N-terminal HA-tagged Ubiquitin is a potent, irreversible and specific inhibitor of most deubiquitinating enzymes (DUBs) including Ubiquitin C-terminal hydrolases (UCHs) and Ubiquitin-specific proteases (USPs). Useful for inhibiting the hydrolysis of poly-Ubiquitin chains on substrate proteins in vitro and thus enhances poly-Ubiquitin chain accumulation.

References
  1. Sharp, P.M. & W.-H. Li. (1987) Trends Ecol. Evol. 2:328.
  2. Ciechanover, A. et al. (1980 ) Proc. Natl. Acad. Sci. USA 77:1365.
  3. Hershko, A. et al. (1980) Proc. Natl. Acad. Sci. USA 77:1783.
  4. Greene, W. et al. (2012) PLoS Pathog. 8:e1002703.
  5. Tong, X. et al. (2012) J. Biol. Chem. 287:25280.
  6. Wei, W. et al. (2004) Nature 428:194.
  7. Wertz, I.E. et al. (2004) Nature 430:694.
Entrez Gene IDs
7314 (Human); 298693 (Rat)
Alternate Names
RPS27A; UBA52; UBB ubiquitin B; UBB; UBC; Ubiquitin

Citations for Recombinant Human HA-Ubiquitin Vinyl Sulfone Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
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  1. Accelerating inhibitor discovery for deubiquitinating enzymes
    Authors: WC Chan, X Liu, RS Magin, NM Girardi, SB Ficarro, W Hu, MI Tarazona G, CA Starnbach, A Felix, G Adelmant, AC Varca, B Hu, AS Bratt, E DaSilva, NJ Schauer, I Jaen Maiso, EK Dolen, AX Ayala, JA Marto, SJ Buhrlage
    Nature Communications, 2023-02-08;14(1):686.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  2. Targeting ubiquitin-specific protease 8 sensitizes anti-programmed death-ligand 1 immunotherapy of pancreatic cancer
    Authors: H Yang, X Zhang, M Lao, K Sun, L He, J Xu, Y Duan, Y Chen, H Ying, M Li, C Guo, Q Lu, S Wang, W Su, T Liang, X Bai
    Cell Death and Differentiation, 2022-12-20;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  3. Targeting USP10 induces degradation of oncogenic ANLN in esophageal squamous cell carcinoma
    Authors: YF Cao, L Xie, BB Tong, MY Chu, WQ Shi, X Li, JZ He, SH Wang, ZY Wu, DX Deng, YQ Zheng, ZM Li, XE Xu, LD Liao, YW Cheng, LY Li, LY Xu, EM Li
    Cell Death and Differentiation, 2022-12-16;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  4. USP1-trapping lesions as a source of DNA replication stress and genomic instability
    Authors: KE Coleman, Y Yin, SKL Lui, S Keegan, D Fenyo, DJ Smith, E Rothenberg, TT Huang
    Nature Communications, 2022-04-01;13(1):1740.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  5. Antibody toolkit reveals N-terminally ubiquitinated substrates of UBE2W
    Authors: CW Davies, SE Vidal, L Phu, J Sudhamsu, TB Hinkle, S Chan Rosen, FR Schumacher, YJ Zeng, C Schwerdtfe, AS Peterson, JR Lill, CM Rose, AS Shaw, IE Wertz, DS Kirkpatric, JT Koerber
    Nature Communications, 2021-07-29;12(1):4608.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  6. Selective USP7 inhibition elicits cancer cell killing through a p53-dependent mechanism
    Authors: NJ Schauer, X Liu, RS Magin, LM Doherty, WC Chan, SB Ficarro, W Hu, RM Roberts, RE Iacob, B Stolte, AO Giacomelli, S Perera, K McKay, SA Boswell, EL Weisberg, A Ray, D Chauhan, S Dhe-Pagano, KC Anderson, JD Griffin, J Li, WC Hahn, PK Sorger, JR Engen, K Stegmaier, JA Marto, SJ Buhrlage
    Sci Rep, 2020-03-24;10(1):5324.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Tumor necrosis factor related apoptosis inducing ligand (TRAIL) regulates deubiquitinase USP5 in tumor cells
    Authors: H Potu, M Kandarpa, LF Peterson, NJ Donato, M Talpaz
    Oncotarget, 2019-10-08;10(56):5745-5754.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  8. A new gold(I) complex-Au(PPh3)PT is a deubiquitinase inhibitor and inhibits tumor growth
    Authors: X Li, Q Huang, H Long, P Zhang, H Su, J Liu
    EBioMedicine, 2018-12-05;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Inhibiting Multiple Deubiquitinases to Reduce Androgen Receptor Expression in Prostate Cancer Cells
    Authors: A de Las Poz, T Reiner, V De Cesare, M Trost, C Perez-Stab
    Sci Rep, 2018-09-03;8(1):13146.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. ZUFSP Deubiquitylates K63-Linked Polyubiquitin Chains to Promote Genome Stability
    Authors: P Haahr, N Borgermann, X Guo, D Typas, D Achuthanku, S Hoffmann, R Shearer, TK Sixma, N Mailand
    Mol. Cell, 2018-03-22;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  11. Reactive-site-centric chemoproteomics identifies a distinct class of deubiquitinase enzymes
    Authors: DS Hewings, J Heideker, TP Ma, AP AhYoung, F El Oualid, A Amore, GT Costakes, D Kirchhofer, B Brasher, T Pillow, N Popovych, T Maurer, C Schwerdtfe, WF Forrest, K Yu, J Flygare, M Bogyo, IE Wertz
    Nat Commun, 2018-03-21;9(1):1162.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  12. Usp9x Promotes Survival in Human Pancreatic Cancer and Its Inhibition Suppresses Pancreatic Ductal Adenocarcinoma In Vivo Tumor Growth
    Authors: A Pal, M Dziubinski, MP Di Maglian, DM Simeone, S Owens, D Thomas, L Peterson, H Potu, M Talpaz, NJ Donato
    Neoplasia, 2017-12-14;20(2):152-164.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  13. Characterization of the deubiquitination activity and substrate specificity of the chicken ubiquitin-specific protease 1/USP associated factor 1 complex
    Authors: H Zheng, M Wang, C Zhao, S Wu, P Yu, Y Lü, T Wang, Y Ai
    PLoS ONE, 2017-11-01;12(11):e0186535.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  14. Usp9x regulates Ets-1 ubiquitination and stability to control NRAS expression and tumorigenicity in melanoma
    Authors: H Potu, LF Peterson, M Kandarpa, A Pal, H Sun, A Durham, PW Harms, PC Hollenhors, U Eskiocak, M Talpaz, NJ Donato
    Nat Commun, 2017-02-15;8(0):14449.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  15. PCNA-Dependent Cleavage and Degradation of SDE2 Regulates Response to Replication Stress
    PLoS Genet, 2016-12-01;12(12):e1006465.
    Species: Human
    Sample Types: Whole Cells
    Applications: Enzyme Assay
  16. Coinhibition of the deubiquitinating enzymes, USP14 and UCHL5, with VLX1570 is lethal to ibrutinib- or bortezomib-resistant Waldenstrom macroglobulinemia tumor cells
    Blood Cancer J, 2016-11-04;6(11):e492.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  17. USP9X stabilizes XIAP to regulate mitotic cell death and chemoresistance in aggressive B-cell lymphoma
    Authors: Katharina Engel
    EMBO Mol Med, 2016-08-01;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  18. Pyk2 Controls Integrin-Dependent CTL Migration through Regulation of De-Adhesion
    J Immunol, 2016-07-25;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Enzyme Assay
  19. Ubiquitination of ROR?t at Lysine 446 Limits Th17 Differentiation by Controlling Coactivator Recruitment
    J Immunol, 2016-07-18;0(0):.
  20. Usp9X Is Required for Lymphocyte Activation and Homeostasis through Its Control of ZAP70 Ubiquitination and PKC? Kinase Activity
    Authors: E Naik, VM Dixit
    J Immunol, 2016-03-02;196(8):3438-51.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  21. Differential role of the proteasome in the early and late phases of BDNF-induced facilitation of LTP.
    Authors: Santos A, Mele M, Vaz S, Kellermayer B, Grimaldi M, Colino-Oliveira M, Rombo D, Comprido D, Sebastiao A, Duarte C
    J Neurosci, 2015-02-25;35(8):3319-29.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  22. Caspase-1 promotes Epstein-Barr virus replication by targeting the large tegument protein deneddylase to the nucleus of productively infected cells.
    Authors: Gastaldello S, Chen X, Callegari S, Masucci M
    PLoS Pathog, 2013-10-10;9(10):e1003664.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Reviews for Recombinant Human HA-Ubiquitin Vinyl Sulfone Protein, CF

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Recombinant Human HA-Ubiquitin Vinyl Sulfone Protein, CF
By Anonymous on 05/17/2016
Application: in vitro dub activity xenopus extract
Reason for Rating: it worked very well but too expensive