Recombinant Human GPR158 His-tag Protein, CF

Catalog #: 10286-GP Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10286-GP-050

Recombinant Human GPR158 His-tag Protein Binding Activity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human GPR158 His-tag Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human GPR158 is immobilized at 1 µg/mL (100 µL/well), it binds to Recombinant Human Neuropilin-1 (Catalog # 3870-N1) with an ED₅₀ of 0.8-4.8 μg/mL

Source

Human embryonic kidney cell, HEK293-derived human GPR158 protein
Ala24-Gln411, with a C-terminal 6-His tag

Accession #

Q5T848

N-terminal Sequence
Analysis

Ala24 & Ser25

Predicted Molecular Mass

45 kDa

SDS-PAGE

50-70 kDa, under reducing conditions

Product Datasheets

10286-GP

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10286-GP

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 1 mg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human GPR158 (Catalog # 10286-GP) is coated at 1 µg/mL, 100 µL/well, Recombinant Human Neuropilin-1 (Catalog # 3870-N1) binds with an ED₅₀ of 0.8-4 µg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human GPR158 His-tag Protein (Catalog # 10286-GP) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 50-70 kDa.

Reconstitution Calculator

Background: GPR158

G-protein coupled receptor 158 (GPR158) is a receptor belonging to the Class C GPCR family. It lacks the extracellular Venus flytrap module characteristic of the known members of that family and instead contains two other elements that are not typical of the class: a calcium-binding EGF-like domain and a leucine repeat region (1, 2). The mature extracellular domain of human GPR158 contains 393 amino acids (aa) and shares 89% identity with both mouse and rat GPR158. GPR158 is expressed at the highest level in the brain, but also in a variety of other tissues including retina, spleen, liver and lung (3). GPR158 was originally identified in functional screens linked with biological stress and has been implicated in the osteocalcin effect on cognitive processes in the brain (4, 5), and glaucoma and cancer in the periphery (4, 6).

References