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Recombinant Human GPR115 Protein, CF

Catalog #: 5437-GP Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
5437-GP-050
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human GPR115 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of U‑87 MG human glioblastoma/astrocytoma cells. When 5 x 104 cells per well are added to rhGPR-115 coated plates (5 μg/mL, 100 μL/well), approximately 60-85% will adhere after 60 minutes at 37 °C.
Source
Chinese Hamster Ovary cell line, CHO-derived human GPR115 protein
Ser22-Ala347, with a C-terminal 6-His tag
Accession #
Q8IZF3
N-terminal Sequence
Analysis
Ser22
Predicted Molecular Mass
37 kDa
SDS-PAGE
66-100 kDa, reducing conditions

Product Datasheets

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5437-GP

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5437-GP

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GPR115

GPR115 is a member of the LN-7TM family of adhesion-type 7-transmembrane (TM) G-protein coupled receptors (GPCR) that show a long extracellular N-terminus (1, 2). The 695 amino acid (aa) human GPR115 sequence predicts a 21 aa signal sequence, a 385 aa N-terminal extracellular domain (ECD), seven TM regions separated by 6 - 24 aa intracellular and extracellular regions, and a 40 aa cytoplasmic tail. Like other LN-7TM members, the ECD contains a highly glycosylated mucin-like stalk that is predicted to function in adhesion. This is followed by a cysteine-rich GPCR proteolytic cleavage site (GPS) (1). GPS domains, which have been described in other 7TM proteins including ETL, GPR126, HE6, and Latrophilin-1, are cleavage sites for processing proteins into two subunits (3 - 7). Within the N terminal region that ends with the predicted cleavage site (aa 22 - 347), human GPR115 shares 58% aa sequence identity with the corresponding region of mouse and rat GPR115. GPR115 was identified from expressed sequence tags (ESTs) found in pregnant uterus, breast, and the genitourinary tract (1).

References
  1. Fredriksson, R. et al. (2002) FEBS Lett. 531:407.
  2. Bjarnadottir, T.K. et al. (2004) Genomics 84:23.
  3. Nechiporuk, T. et al. (2001) J. Biol. Chem. 276:4150.
  4. Moriguchi, T. et al. (2004) Genes Cells 9:549.
  5. Kierszenbaum, A.L. (2003) Mol. Reprod. Dev. 64:1.
  6. Krasnoperov, V.G. et al. (1997) Neuron 18:925.
  7. Krasnoperov, V. et al. (2002) J. Biol. Chem. 277:46518.
Long Name
G Protein-coupled Receptor 115
Entrez Gene IDs
221393 (Human); 78249 (Mouse); 501106 (Rat)
Alternate Names
FLJ38076; G protein-coupled receptor 115; GPR115; G-protein coupled receptor PGR18; PGR18; PGR18G-protein coupled receptor 115; probable G-protein coupled receptor 115; seven transmembrane helix receptor

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