Recombinant Human Glypican 3 His-tag Avi-tag Protein, CF

Catalog #: AVI2119 Datasheet / COA / SDS

Biotinylated

Catalog #	Availability	Size / Price	Qty
AVI2119-050

Recombinant Human Glypican 3 His-tag Avi-tag Protein Binding Activity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Glypican 3 His-tag Avi-tag Protein, CF Summary

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human FGF basic/FGF2 (Catalog # 233-FB/CF) is immobilized at 0.1 µg/mL (100 µL/well), Biotinylated Recombinant Human Glypican 3 His-tag Avi-tag (Catalog # AVI2119) binds with an ED₅₀ of 20-100 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human Glypican 3 protein

Human Glypican 3 (Gln25-His559) Accession # P51654.1	HHHHHH	Avi-tag
N-terminus		C-terminus

Accession #

P51654.1

N-terminal Sequence
Analysis

Sequence confirmed by mass spectrometry.

Structure / Form

Biotinylated via Avi-tag

Predicted Molecular Mass

63 kDa

SDS-PAGE

90-140 kDa under non-reducing conditions.

Product Datasheets

AVI2119

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI2119

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human FGF basic/FGF2/bFGF (146aa) Protein (233-FB/CF) is immobilized at 0.1 μg/mL (100 μL/well), Biotinylated Recombinant Human Glypican-3 His-tag Avi-tag (AVI2119) binds with an ED₅₀ of 20-120 ng/mL.

Reconstitution Calculator

Background: Glypican 3

Glypican 3 (GPC3), also known as GTR2-2, intestinal protein OCI-5 and MXR7, is a member of the heparan sulfate (HS) proteoglycan family (1). In mammals, six glypican family members have been identified, all sharing a conserved core protein that is linked to the cell membrane by a glycosylphosphatidylinositol (GPI) anchor. GPC3 can be cleaved by furin to produce two subunits that are linked by disulfide bonds: a 40 kDa N-terminal alpha subunit that can be secreted into the blood and a 30 kDa membrane-bound C-terminal beta subunit containing two HS glycan chains (1-3). Several isoforms of GPC3 due to alternative splicing have been reported (1). Within the mature extracellular domain, human GPC3 shares 96% amino acid sequence identity with both mouse and rat GPC3. GPC3 is an important biomarker present in the serum of hepatocellular carcinoma patients, which distinguishes benign from cancerous nodules (4). Glypican 3 is over-expressed in hepatocellular carcinomas and binding of GPC3 to CD81 promotes development of carcinomas by activation of Hippo pathways in hepatocytes (5). Our Avi-tag Biotinylated Recombinant Glypican 3 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

Ho, M. and Kim, H. (2012) Eur. J. Cancer. 47:333.
Haruyama, Y. and Kataoka, H. (2016) World J. Gastroenterol. 22:275.
Shimizu, Y. et al. (2019) Front Oncol. 9:248.
Ge, S. et al. (2018) Filmus, Int J Clin Exp Pathol. 11(12):5774.
Xue, Y. et al. (2018) Am J Pathol. 188(6):1469.

Entrez Gene IDs

2719 (Human); 14734 (Mouse); 25236 (Rat); 102137748 (Cynomolgus Monkey)

Alternate Names

DGSX; Glypican 3; glypican proteoglycan 3; glypican-3; GPC3; GTR2-2; heparan sulphate proteoglycan; Intestinal protein OCI-5; MXR7; OCI5; OCI-5; secreted glypican-3; SGB; SGBS; SGBS1SDYS