Recombinant Human FGFR3 (IIIc) Fc Chimera Protein, CF

Catalog #: 766-FR
4 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
766-FR-050

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Citations (4)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human FGFR3 (IIIc) Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.1 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit FGF acidic-dependent proliferation of NR6R‑3T3 mouse fibroblast cells. The ED₅₀ for this effect is 1‑3 ng/mL.

Source

Mouse myeloma cell line, NS0-derived human FGF R3 protein

Human FGF R3 (IIIc) (Glu23-Gly375) Accession # P22607	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

P22607

N-terminal Sequence
Analysis

Glu23

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

64.7 kDa (monomer)

SDS-PAGE

95-105 kDa, reducing conditions

Product Datasheets

766-FR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

766-FR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: FGFR3

Fibroblast growth factors (FGFs) comprise a family of at least eighteen structurally related proteins that are involved in a multitude of physiological and pathological cellular processes, including cell growth, differentiation, angiogenesis, wound healing and tumorgenesis. The biological activities of the FGFs are mediated by a family of type I transmembrane tyrosine kinases which undergo dimerization and autophosphorylation after ligand binding. Four distinct genes encoding closely related FGF receptors, FGF R1-4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid‑box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain. Multiple forms of FGF R1‑3 are generated by alternative splicing of the mRNAs. A frequent splicing event involving FGF R1 and 2 results in receptors containing all three Ig domains, referred to as the alpha isoform, or only IgII and IgIII, referred to as the beta isoform. Only the alpha isoform has been identified for FGF R3 and FGF R4. Additional splicing events for FGF R1-3, involving the C-terminal half of the IgIII domain encoded by two mutually exclusive alternative exons, generate FGF receptors with alternative IgIII domains (IIIb and IIIc). A IIIa isoform which is a secreted FGF binding protein containing only the N-terminal half of the IgIII domain plus some intron sequences has also been reported for FGF R1. Mutations in FGF R1-3 have been found in patients with birth defects involving craniosynostosis. The complex patterns of expression of these receptors as well as the specificity of their interactions with the various FGF ligand family members are under investigation.

References

Galzie, Z. et al. (1997) Biochem. Cell Biol. 75:669.
Burke, D. et al. (1998) Trends Biochem. Sci. 23:59.

Long Name

Fibroblast Growth Factor Receptor 3

Entrez Gene IDs

2261 (Human); 14184 (Mouse)

Alternate Names

CD333; CEK; EC 2.7.10; FGF R3; FGFR3; fibroblast growth factor receptor 3; HSFGFR3EX; JTK4; JTK4thanatophoric dwarfism

Related Research Areas

Citations for Recombinant Human FGFR3 (IIIc) Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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FGF21-FGFR4 signaling in cardiac myocytes promotes concentric cardiac hypertrophy in mouse models of diabetes
Authors: C Yanucil, D Kentrup, X Li, A Grabner, K Schramm, EC Martinez, J Li, I Campos, B Czaya, K Heitman, D Westbrook, AR Wende, A Sloan, JM Roche, A Fornoni, MS Kapiloff, C Faul
Scientific Reports, 2022-05-05;12(1):7326.
Species: Human
Sample Types: Recombinant Proteins
Applications: Bioassay
Semi-Automated Cell Panning for Efficient Isolation of FGFR3-Targeting Antibody
Authors: B Min, M Yoo, H Kim, M Cho, DH Nam, Y Yoon
International Journal of Molecular Sciences, 2021-06-09;22(12):.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay
2-O Heparan Sulfate Sulfation by Hs2st Is Required for Erk/Mapk Signalling Activation at the Mid-Gestational Mouse Telencephalic Midline.
Authors: Chan W, Howe K, Clegg J, Guimond S, Price D, Turnbull J, Pratt T
PLoS ONE, 2015-06-15;10(6):e0130147.
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay
Targeting the extracellular domain of fibroblast growth factor receptor 3 with human single-chain Fv antibodies inhibits bladder carcinoma cell line proliferation.
Authors: Martinez-Torrecuadrada J, Cifuentes G, Lopez-Serra P, Saenz P, Martinez A, Casal JI
Clin. Cancer Res., 2005-09-01;11(17):6280-90.
Species: Human
Sample Types: Antibody
Applications: Surface Plasmon Resonance