Recombinant Human FGFR3 alpha (IIIb) Fc Avi-tag Protein, CF

Catalog #: AVI11029 Datasheet / COA / SDS

Biotinylated

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AVI11029-050

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human FGFR3 alpha (IIIb) Fc Avi-tag Protein, CF Summary

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. In a Human FGF acidic/FGF1 antibody (Catalog # AF232) coated plate, in the presence of 50.0 ng/mL of Recombinant Human FGF acidic/FGF1, Biotinylated Recombinant Human FGFR3 alpha (IIIb) Fc Chimera Avi-tag Protein binds with an ED₅₀ of 40.0-240 ng/mL.

Source

Human embryonic kidney cell, HEK293-derived human FGFR3 protein

Human FGFR3 alpha (IIIB) (Glu23-Gly377) Accession # NP_001156685.1	IEGRMD	Human IgG₁ Fc (Pro100-Lys330)	Avi-tag
N-terminus			C-terminus

Accession #

NP_001156685.1

N-terminal Sequence
Analysis

Glu23

Structure / Form

Disulfide-linked homodimer

Biotinylated via Avi-tag

Predicted Molecular Mass

67 kDa

SDS-PAGE

92 - 104 kDa, under reducing conditions.

Product Datasheets

AVI11029

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI11029

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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In a Human FGFacidic/FGF1 antibody (AF232) coated plate, in the presence of 50 ng/mL of Recombinant Human FGFacidic/FGF1 (232-FA), Recombinant Human FGFR3 alpha (IIIb) Fc Chimera Avi-tag Protein (Catalog # AVI11029) binds with an ED₅₀ of 40.0-240 ng/mL.

SDS-PAGE

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2 μg/lane of Biotinylated Recombinant Human FGFR3 (IIIb) Fc Chimera Avi-tag Protein (Catalog # AVI11029) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 92-104 kDa and 184-208 kDa, respectively.

Reconstitution Calculator

Background: FGFR3

Fibroblast growth factor receptor 1 (FGFR1) belongs to a family of type I transmembrane tyrosine kinases which mediate the biological functions of FGFs that are involved in a multitude of physiological and pathological cellular processes (1). The FGFR family is comprised of 4 structurally conserved members (FGFR1-4) all possessing and extracellular domain (ECD) with three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain (1, 2). The ECD of mature, full-length FGFR3 shares 92% amino acid sequence identity with mouse FGFR3. Alternative splicing generates multiple forms of FGFR1-3, each with unique signaling characteristics (1-3). For FGFR3, alternative splicing of the ECD, specifically the IgIII domain, results in IIIb, or IIIc isoforms (4). The FGFR splice variants also exhibit distinct and varying binding affinities for different FGF ligands (2). FGFRs mediate the FGF signaling cascade which regulate developmental processes including cellular proliferation, differentiation, and migration, morphogenesis, and patterning (5). FGFRs transduce the signals through three dominant pathways including RAS/MAPK, PI3k/AKT, and PLC gamma (6). FGFR3 is normally expressed in the tissues of the central nervous system, brain, kidney and testis, with the FGFR3A(IIIb) splice variant predominantly expressed in epithelial cells (7, 8). FGFR3 signaling is critical for bone growth regulation and mutations in FGFR3 or misregulation of FGFR3 mediated signaling is found in multiple skeletal dysplasias, with FGFR3A(IIIb) specifically upregulated in hepatocellular carcinoma but down regulated in colorectal cancer (1,4, 8, 9). Our Avi-tag Biotinylated FGFR3A(IIIb) features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References