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Recombinant Human Endosialin/CD248 Protein, CF

Catalog #: 7855-CD
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
7855-CD-050
R&D Systems Recombinant Proteins and Enzymes
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Citations (2)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Endosialin/CD248 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human Endosialin/CD248 is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Human Galectin‑3BP/MAC‑2BP (Catalog # 2226-GA) that produces 50% of the optimal binding response is approximately 0.08-0.4 μg/mL.
Source
Mouse myeloma cell line, NS0-derived human Endosialin/CD248 protein
Gln18-Arg685, with a C-terminal 6-His tag
Accession #
Q9HCU0
N-terminal Sequence
Analysis
Gln18 predicted: No results obtained, sequencing might be blocked
Predicted Molecular Mass
72 kDa
SDS-PAGE
110-150 kDa, reducing conditions

Product Datasheets

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7855-CD

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7855-CD

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Endosialin/CD248

Endosialin, also known as TEM1 (tumor endothelial marker 1) and designated CD248, is a 165‑175 kDa type I transmembrane O‑glycosylated protein that is expressed on activated perivascular and stromal cells in embyronic and tumor neovasculature, but down‑regulated in quiescent vasculature (1‑4). The human Endosialin cDNA encodes 757 amino acids (aa) including a 17 aa signal sequence, a 670 aa extracellular domain (ECD) with one C‑type lectin, one Sushi, one EGF‑like domain and a mucin-like stalk, a transmembrane sequence, and a short (49 aa) cytoplasmic domain with three potential phosphorylation sites and a PDZ binding motif (2, 3). Within the ECD, human Endosialin shares 76% and 74% aa sequence identity with mouse and rat Endosialin, respectively. Endosialin regulates pericyte proliferation, migration, and adhesion to endothelial cell‑deposited fibronectin and collagens I and IV in the extracellular matrix (3‑7). It binds Galectin‑3/MAC‑2BP, mediating a repulsive signal thought to guide tumor cell migration (5). In blood vessel development, it promotes endothelial cell apoptosis and pruning of unwanted capillaries (6). In humans, it is up‑regulated on mesenchymal stem cells, naïve CD8+ T cells, and leukocytes that express markers of endothelial and dendritic lineages (3, 8). It is dynamically expressed on lymphoid tissue stromal cells and is required for lymph node, spleen, and thymus remodeling during immune responses (8‑11). Its expression correlates with suppressed proliferation of human naïve CD8+ T cells, and with aggressiveness of some tumors (8, 12, 13). Endosialin expression is up‑regulated in vitro by high cell density and hypoxia (3, 14). Inflammatory conditions such as rheumatoid and psoriatic arthritis and IgA nephropathy can cause expression in stroma that do not normally express Endosialin (3).

References
  1. Rettig, W.J. et al. (1992) Proc. Natl. Acad. Sci. USA 89:10832.
  2. Christian, S. et al. (2001) J. Biol. Chem. 276:7408.
  3. Valdez, Y. et al. (2012) Curr. Drug Targets 13:432.
  4. MacFadyen, J.R. et al. (2005) FEBS Lett. 579:2569.
  5. Becker, R. et al. (2008/) FASEB J. 22:3059.
  6. Simonavicius, N. et al. (2012) Blood 120:1516.
  7. Tomkowicz, B. et al. (2010) Cancer Biol. Ther. 9:908.
  8. Hardie, D.L. et al. (2011) Immunology 133:288.
  9. Lax, S. et al. (2007) FEBS Lett. 581:3551.
  10. Lax, S. et al. (2010) Eur. J. Immunol. 40:1884.
  11. Lax, S. et al. (2012) FEBS Open Bio 2:187.
  12. Nanda, A. et al. (2006) Proc. Natl. Acad. Sci. USA 103:3351.
  13. Maia, M. et al. (2011) BMC Cancer 11:162.
  14. Ohradanova, A. et al. (2008) Br. J. Cancer 99:1348.
Entrez Gene IDs
57124 (Human); 70445 (Mouse); 293669 (Rat)
Alternate Names
2610111G01Rik; CD164 sialomucin-like 1; CD164L1; CD248 antigen; CD248 antigen, endosialin; CD248 molecule, endosialin; CD248; Endosialin; MGC119479; TEM1; TEM1MGC119478; Tumor endothelial marker 1

Citations for Recombinant Human Endosialin/CD248 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Targeting fibroblast CD248 attenuates CCL17-expressing macrophages and tissue fibrosis
    Authors: CH Pai, SR Lin, CH Liu, SY Pan, H Hsu, YT Chen, CT Yen, IS Yu, HL Wu, SL Lin, SW Lin
    Sci Rep, 2020-10-08;10(1):16772.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Generation of a novel Antibody-Drug Conjugate targeting endosialin: potent and durable antitumor response in sarcoma
    Authors: E Capone, E Piccolo, I Fichera, P Ciufici, D Barcaroli, A Sala, V De Laurenz, V Iacobelli, S Iacobelli, G Sala
    Oncotarget, 2017-07-22;8(36):60368-60377.
    Applications: Bioassay

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