Recombinant Human EGFR Isoform vIII Fc Avi-tag Protein, CF

Catalog #: AVI10494
2 Citations Datasheet / COA / SDS

Biotinylated

Catalog #	Availability	Size / Price	Qty
AVI10494-050

Recombinant Human EGFR Isoform vIII Fc Avi-tag Protein Binding Activity

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Product Details

Citations (2)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human EGFR Isoform vIII Fc Avi-tag Protein, CF Summary

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Human EGFR Isoform vIII antibody (Novus Catalog # 50599) is immobilized at 0.1 µg/mL (100 µL/well), Biotinylated Recombinant Human EGFR Isoform vIII Fc Chimera Avi-tag (Catalog # AVI10494)binds with an ED₅₀ of 25-150 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human EGFR protein

Human EGFR Isoform vIII (Leu25-Ser378) Accession # NP_001333870.1	IEGRMD	Human IgG₁ (Pro100-Lys330)	Avi-tag
N-terminus			C-terminus

Accession #

NP_001333870.1

N-terminal Sequence
Analysis

Leu25

Structure / Form

Disulfide-linked homodimer, biotinylated via Avi-tag

Predicted Molecular Mass

67 kDa

SDS-PAGE

95-115 kDa, under reducing conditions

Product Datasheets

AVI10494

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI10494

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Human EGFR Isoform vIII antibody (Novus Catalog # BNP-50599) is immobilized at 0.1 μg/mL (100 μL/well), Biotinylated Recombinant Human EGFR Isoform vIII Fc Chimera Avi-tag (AVI10494) binds with an ED₅₀ of 25-150 ng/mL.

SDS-PAGE

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2 μg/lane of Biotinylated Recombinant Human EGFR Isoform vIII Fc Avi-tag Protein (AVI10494) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 95-115 kDa and 190-230 kDa, respectively.

Reconstitution Calculator

Background: EGFR

Epidermal growth factor receptor (EGFR), also known as HER-1 and ErbB1, is a member of a subfamily of receptor tyrosine kinases comprised of four members: EGFR, ErbB2 (Neu, HER-2), ErbB3 (HER-3), and ErbB4 (HER-4). All family members are type I transmembrane glycoproteins with an extracellular domain (ECD) containing two cysteine-rich domains separated by a spacer region and a cytoplasmic domain containing a tyrosine kinase domain followed by multiple tyrosine autophosphorylation sites (1, 2). Several soluble isoforms lacking the intracellular domain are generated by alternate splicing (3‑4). EGFRvIII is a tumor‐specific mutation that results from an in‐frame deletion removing 267 amino acids from the ECD and insertion of a glycine residue (5). EGFRvIII has a molecular mass of approximately 145 kDa and has been shown to have weaker activity than full-length EGFR (6). EGFR binds a subset of the EGF family ligands, including EGF, amphiregulin, TGF-alpha, betacellulin, epiregulin, HB-EGF, and epigen (1, 2). Ligand binding induces EGFR homodimerization as well as heterodimerization with ErbB2, resulting in kinase activation, heterodimerization tyrosine phosphorylation and cell signaling (7‑9). EGFR can also be recruited to form heterodimers with the ligand‑activated ErbB3 or ErbB4. EGFR signaling regulates multiple biological functions including cell proliferation, differentiation, motility, and apoptosis (7-9). EGFR is overexpressed in a wide variety of tumors, with EGFRvIII overexpressed particularly in glioblastoma multiforme (GMB), and is the target of several anti-cancer therapeutics (5,10,11). Our Avi-tag Biotinylated Recombinant Human EGFRvIII features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

Singh, A.B. and R.C. Harris (2005) Cell. Signal. 17:1183.
Shilo, B.Z. (2005) Development 132:4017.
Guillaudeau, A. et al. (2012) PLoS One. 7:1.
Reiter J.L. et al. (2001) Genomics 71:1.
Gan HK et al. (2013) FEBS J. 280:5350
Batra SK, et al. (1995) Cell Growth Differ 6:1251
Freed, D. M. et al. (2017) Cell. 171:683.
Burgess, A.W. et al. (2003) Mol. Cell 12:541.
Faria, J. A. et al. (2016) BBRC. 478:39.
An Z. et al. (2018) Oncogene. 37:1561.
Lee, C. K. et al. (2017) J. Thoracic Oncology. 12:403.

Long Name

Epidermal Growth Factor Receptor

Entrez Gene IDs

1956 (Human); 13649 (Mouse); 24329 (Rat); 102138724 (Cynomolgus Monkey)

Alternate Names

avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10; EC 2.7.10.1; EGF R; EGFR; epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog); epidermal growth factor receptor; ErbB; ErbB1; ERBB1PIG61; HER1; HER-1; mENA; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

Related Research Areas

Citations for Recombinant Human EGFR Isoform vIII Fc Avi-tag Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Intrathecal bivalent CAR T cells targeting EGFR and IL13R?2 in recurrent glioblastoma: phase 1 trial interim results
Authors: Bagley, SJ;Logun, M;Fraietta, JA;Wang, X;Desai, AS;Bagley, LJ;Nabavizadeh, A;Jarocha, D;Martins, R;Maloney, E;Lledo, L;Stein, C;Marshall, A;Leskowitz, R;Jadlowsky, JK;Christensen, S;Oner, BS;Plesa, G;Brennan, A;Gonzalez, V;Chen, F;Sun, Y;Gladney, W;Barrett, D;Nasrallah, MP;Hwang, WT;Ming, GL;Song, H;Siegel, DL;June, CH;Hexner, EO;Binder, ZA;O'Rourke, DM;
Nature medicine
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
A Rational Designed Novel Bispecific Antibody for the Treatment of GBM
Authors: R Sun, Y Zhou, L Han, Z Pan, J Chen, H Zong, Y Bian, H Jiang, B Zhang, J Zhu
Biomedicines, 2021-06-03;9(6):.
Species: Human
Sample Types:
Applications: Surface Plasmon Resonance (SPR