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Recombinant Human Desmoglein-2 Fc Chimera Protein, CF

Catalog #: 947-DM
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
947-DM-100
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Desmoglein-2 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of BUD‑8 human fibroblast cells. When 3 x 104 cells are added to recombinant human Desmoglein‑2 coated plates (10 μg/mL, 100 μL/well), approximately 50%‑80% will adhere after 1 hour at 37°C.  Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived human Desmoglein-2 protein
Human Desmoglein-2
(Ala49-Gly608)
Accession # CAA81226		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
CAA81226
N-terminal Sequence
Analysis
Ala49
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
89 kDa (monomer)
SDS-PAGE
115 kDa, reducing conditions

Product Datasheets

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947-DM

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

947-DM

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Desmoglein-2

Desmoglein-2 (DSG2) is a 160 kDa transmembrane glycoprotein in the cadherin family of calcium dependent adhesion molecules. It is a major protein component of desmosomal contacts between epithelial cells (1, 2). Mature human Desmoglein-2 consists of a 560 amino acid (aa) extracellular domain (ECD) with four cadherin domains, a 25 aa transmembrane segment, and a 484 aa cytoplasmic domain with six desmoglein repeats (3). Within the ECD, human Desmoglein-2 shares 81% aa sequence identity with mouse and rat Desmoglein-2. A 60 kDa ECD fragment can be proteolytically shed, and this is enhanced by EGF R activation (4‑6). The cytoplasmic domain is cleaved during apoptosis, leaving the transmembrane segment and ECD as a cell-associated fragment (7). Desmoglein-2 is widely expressed in epithelia and is required for maintenance of the epithelial barrier (1, 2, 8). It can be either up- or down-regulated in a variety of carcinomas during tumor progression (9). Desmoglein-2 also functions as a cell adhesion receptor for several adenovirus serotypes (10). This binding disrupts intercellular junctions between epithelial cells and promotes epithelial-mesenchymal transition (EMT) (10). Mutations in Desmoglein-2 cause cardiac dysfunction typical of arrythmogenic right ventricular cardiomyopathy (ARVC) (11).

References
  1. Brooke, M.A. et al. (2012) J. Pathol. 226:158.
  2. Thomason, H.A. et al. (2010) Biochem. J. 429:419.
  3. Schafer, S. et al. (1994) Exp. Cell Res. 211:391.
  4. Bech-Serra, J.J. et al. (2006) Mol. Cell. Biol. 26:5086.
  5. Klessner, J.L. et al. (2009) Mol. Biol. Cell 20:328.
  6. Ramani, V.C. et al. (2008) BMC Cancer 8:373.
  7. Nava, P. et al. (2007) Mol. Biol. Cell 18:4565.
  8. Schlegel, N. et al. (2010) Am. J. Physiol. Gastrointest. Liver Physiol. 298:G774.
  9. Dusek, R.L. and L.D. Attardi (2011) Nat. Rev. Cancer 11:317.
  10. Wang, H. et al. (2011) Nat. Med. 17:96.
  11. Pilichou, K. et al. (2009) J. Exp. Med. 206:1787.
Entrez Gene IDs
1829 (Human); 13511 (Mouse)
Alternate Names
ARVC10; ARVD10; Cadherin family member 5; CDHF5; CDHF5MGC117034; CMD1BB; desmoglein 2; Desmoglein2; Desmoglein-2; DSG2; HDGC; MGC117036; MGC117037

Citation for Recombinant Human Desmoglein-2 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Diversity within the adenovirus fiber knob hypervariable loops influences primary receptor interactions
    Authors: AT Baker, A Greenshiel, L Coughlan, JA Davies, H Uusi-Kertt, DK Cole, PJ Rizkallah, AL Parker
    Nat Commun, 2019-02-14;10(1):741.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance

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