Home / DC-SIGNR/CD299 / Recombinant Human DC-SIGNR/CD299 Fc Chimera Protein, CF

Recombinant Human DC-SIGNR/CD299 Fc Chimera Protein, CF

Catalog #: 162-D2
6 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
162-D2-050
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Citations (6)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human DC-SIGNR/CD299 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>80%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of ICAM-3 expressing CHO Chinese hamster ovary cells. When 5 x 104 cells/well are added to rhDC-SIGNR/Fc Chimera coated plates (5 µg/mL with 100 µL/well), approximately 40%-70% of added cells will adhere after 1 hour at room temperature.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived human DC-SIGNR/CD299 protein
MD Human IgG1
(Pro100-Lys330)		 IEGR Human DC-SIGNR
(Ser73-Glu376)
Accession # NP_001138382
N-terminus C-terminus
Accession #
NP_001138382
N-terminal Sequence
Analysis
Met
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
61.4 kDa (monomer)
SDS-PAGE
61-66 kDa, reducing conditions

Product Datasheets

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162-D2

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

162-D2

Formulation Lyophilized from a 0.2 μm filtered solution in Tris-Citrate.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DC-SIGNR/CD299

Human Dendritic Cell-specific ICAM-3 Grabbing Non-integrin (DC-SIGN)/CD209 is a member of the C-type lectin family (1). The canonical DC-SIGN/CD209 isoform is a 46 kDa, 404 amino acid (aa) type II transmembrane protein (2). The extracellular region contains a Ca2+-dependent carbohydrate-binding lectin domain (2). Multiple human DC-SIGN/CD209 splice forms exist, generating both membrane-bound and soluble forms (3). DC-SIGN/CD209 is not well conserved between mouse and human, with the extracellular domain sharing only 63% aa identity. The DC-SIGN/CD209 lectin domain binds mannose oligosaccharides on pathogens including HIV as well as self glycoproteins including ICAMs (2, 4). DC-SIGN/CD209 is expressed on dendritic cells (DC) and inflammatory macrophages and contributes to antigen presentation (5, 6).

References
  1. Liu, W. et al. (2004) J. Biol. Chem. 279:18748.
  2. Curtis, B.M. et al. (1992) Proc. Natl. Acad. Sci. USA 89:8356.
  3. Mummidi, S. et al. (2001) J. Biol. Chem. 276:33196.
  4. Anthony, R.M. et al. (2008) Proc. Natl. Acad. Sci. USA 105:19571.
  5. Geijtenbeek, T.B. et al. (2000) Cell 100:575.
  6. Garcia-Vallejo, J.J. and Y. van Kooyk (2013) Trends Immunol. 34:482.
Long Name
Dendritic Cell-specific ICAM-3-grabbing Non-integrin 2
Entrez Gene IDs
10332 (Human)
Alternate Names
CD209L; CD209L1; CD209Ldendritic cell-specific ICAM-3-grabbing nonintegrin 2; CD299 antigenMGC129964; CD299; CLEC4M; C-type lectin domain family 4, member M; DC-SIGN2; DC-SIGN2CD209 antigen-like protein 1; DCSIGNR; DC-SIGNR; DC-SIGNRDC-SIGN-related protein; DCSIGNRMGC47866; Dendritic cell-specific ICAM-3-grabbing non-integrin 2; HP10347; liver/lymph node-specific ICAM-3 grabbing non-integrin; Liver/lymph node-specific ICAM-3-grabbing non-integrin; LSIGN; L-SIGN; LSIGNC-type lectin domain family 4 member M; mannose binding C-type lectin DC-SIGNR

Citations for Recombinant Human DC-SIGNR/CD299 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Overexpression of Ebola virus envelope GP1 protein
    Authors: Z Zou, J Misasi, N Sullivan, PD Sun
    Protein Expr. Purif., 2017-04-27;0(0):.
    Species: Virus - Ebola
    Sample Types: Protein
    Applications: Enzyme Assay
  2. DC - SIGNR by influencing the lncRNA HNRNPKP2 upregulates the expression of CXCR4 in gastric cancer liver metastasis
    Authors: Y Zhang, Q Zhang, M Zhang, M Yuan, Z Wang, J Zhang, X Zhou, Y Zhang, F Lin, H Na, S Ren, Y Zuo
    Mol. Cancer, 2017-04-13;16(1):78.
    Species: Human
    Sample Types: Serum
    Applications: ELISA (Standard)
  3. Developing the IVIG biomimetic, hexa-Fc, for drug and vaccine applications.
    Authors: Czajkowsky D, Andersen J, Fuchs A, Wilson T, Mekhaiel D, Colonna M, He J, Shao Z, Mitchell D, Wu G, Dell A, Haslam S, Lloyd K, Moore S, Sandlie I, Blundell P, Pleass R
    Sci Rep, 2015-04-27;5(0):9526.
    Applications: Bioassay
  4. The clinical significance of DC-SIGN and DC-SIGNR, which are novel markers expressed in human colon cancer.
    Authors: Jiang, Yanmei, Zhang, Changfu, Chen, Kai, Chen, Zhe, Sun, Zhigang, Zhang, Zhuqing, Ding, Dongbing, Ren, Shuangyi, Zuo, Yunfei
    PLoS ONE, 2014-12-12;9(12):e114748.
    Species: Human
    Sample Types: Serum
    Applications: ELISA (Standard)
  5. O-mannosylation of the Mycobacterium tuberculosis adhesin Apa is crucial for T cell antigenicity during infection but is expendable for protection.
    Authors: Nandakumar, Subhadra, Kannanganat, Sunil, Dobos, Karen M, Lucas, Megan, Spencer, John S, Fang, Sunan, McDonald, Melissa, Pohl, Jan, Birkness, Kristin, Chamcha, Venkates, Ramirez, Melissa, Plikaytis, Bonnie B, Posey, James E, Amara, Rama Rao, Sable, Suraj B
    PLoS Pathog, 2013-10-10;9(10):e1003705.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Respiratory syncytial virus glycoprotein G interacts with DC-SIGN and L-SIGN to activate ERK1 and ERK2.
    Authors: Johnson TR, McLellan JS, Graham BS
    J. Virol., 2011-11-16;86(3):1339-47.
    Applications: Bioassay

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