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Recombinant Human CXCL3/GRO gamma (aa 39-107) Protein

Catalog #: 8297-GG Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
8297-GG-025/CF

With Carrier

Catalog # Availability Size / Price Qty
8297-GG-025
Recombinant Human CXCL3/GRO gamma (aa 39-107) Protein Bioactivity
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CXCL3/GRO gamma (aa 39-107) Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE with silver staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR2. The ED50 for this effect is 0.4-2.4 ng/mL.
Source
E. coli-derived human CXCL3/GRO gamma/CINC-2/DCIP-1 protein
Thr39-Asn107
Accession #
P19876
N-terminal Sequence
Analysis
Thr39
Predicted Molecular Mass
8 kDa
SDS-PAGE
7 kDa, reducing conditions

Product Datasheets

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8297-GG (with carrier)

Product Datasheet
COA
SDS

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8297-GG/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8297-GG

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 250 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

8297-GG/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Human CXCL3/GRO gamma (aa 39-107) Protein Bioactivity View Larger

Recombinant Human CXCL3/GRO gamma (Catalog # 8297-GG) chemoattracts BaF3 mouse pro B cells transfected with human CXCR2. The ED50 for this effect is 0.4-2.4 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CXCL3/GRO gamma/CINC-2/DCIP-1

CXCL3, also known as Growth-regulated Oncogene gamma (GRO gamma), Macrophage Inflammatory Protein 2 beta (MIP-2beta), Dendritic Cell Inflammatory Protein 1 (DCIP-1), or Cytokine-induced Neutrophil Attractant 2 (CINC-2), is an 8 kDa pro-inflammatory member of the CXC subfamily of heparin-binding chemokines (1-3). Mature human CXCL3 (aa 35-107) shares 70% and 74% amino acid (aa) sequence identity with mouse and rat CXCL3, respectively. CXCL3 binds and activates CXCR2 to induce chemoattraction of CXCR2-expressing cells including neutrophils and endothelial cells (4, 5). Additional N-terminal processing of mature CXCL3 by the removal of aa 35-38 increases its chemotactic activity by several fold (6). In addition to binding CXCR2, CXCL3 can also bind and be sequestered by the Duffy Antigen Receptor for Chemokines (DARC) decoy receptor (7).

References
  1. Tekamp-Olson, P. et al. (1990) J. Exp. Med. 172:911.
  2. Haskill, S. et al. (1990) Proc. Natl. Acad. Sci. USA 87:7732.
  3. Zlotnik, A. and O. Yoshie (2012) Immunity 36:705.
  4. Strydom, N. and S.M. Rankin (2013) J. Innate Immun. 5:304.
  5. Mehrad, B. et al. (2007) Thromb. Haemost. 97:755.
  6. Wuyts, A. et al. (1999) Eur. J. Biochem. 260:421.
  7. Hansell, C.A. et al. (2011) Immunol. Cell Biol. 89:197.
Entrez Gene IDs
2921 (Human); 330122 (Mouse); 171551 (Rat)
Alternate Names
chemokine (C-X-C motif) ligand 3; CINC-2; CINC-2b; C-X-C motif chemokine 3; CXCL3; Dcip1; Gm1960; GRO gamma; GRO3 oncogene; GRO3; GROG; GRO-gamma; GRO-gamma(1-73); Growth-regulated protein gamma; Macrophage inflammatory protein 2-beta; melanoma growth stimulatory activity gamma; MGSA gamma; MIP2B; MIP-2b; MIP2-beta; SCYB3

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