Recombinant Human CRTAC1 Protein, CF Summary
Product Specifications
Ser28-Cys661, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
5234-CR
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 250 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: CRTAC1
Cartilage acidic protein 1 (CRTAC1), also known as CEP‑68, is a 95 kDa glycosylated protein that contains four FG‑GAP domains and one EGF‑like domain. An integrin‑binding RGD motif is present in the human protein but absent in the mouse and rat proteins. Mature human CRTAC1 shares 89% amino acid sequence identity with mouse and rat CRTAC. It is secreted by chondrocytes into the extracellular matrix of cartilage (1, 2). It is also up‑regulated in type II alveolar epithelial cells upon dexamethasone exposure (3). CRTAC1B, otherwise known as LOTUS, is an 85 kDa alternatively spliced isoform that has a substituted C‑terminal region with a potential transmembrane segment (2, 4). CRTAC1B is preferentially expressed in select regions of the brain on neuronal growth cones and neurite shafts (2, 4). It associates with the Nogo Receptor (NgR) and prevents Nogo‑induced growth cone collapse (4, 5). In mouse, CRTAC1B plays an important role in axonal bundling and formation of the lateral olfactory tract (4).
- Steck, E. et al. (2001) Biochem. J. 353:169.
- Steck, E. et al. (2007) Matrix Biol. 26:30.
- Ballard, P.L. et al. (2010) Am. J. Physiol. Lung Cell. Mol. Physiol. 299:L36.
- Sato, Y. et al. (2011) Science 333:769.
- Kurihara, Y. et al. (2012) Biochem. Biophys. Res. Commun. 418:390.
Citation for Recombinant Human CRTAC1 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Decreased plasma cartilage acidic protein 1 in COVID-19
Authors: Johansson, MW;Balnis, J;Muehlbauer, LK;Bukhman, YV;Stefely, MS;Overmyer, KA;Vancavage, R;Tiwari, A;Adhikari, AR;Feustel, PJ;Schwartz, BS;Coon, JJ;Stewart, R;Jaitovich, A;Mosher, DF;
Physiological reports
Species: Human
Sample Types:
Applications: ELISA Standard
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