Recombinant Human Common gamma Chain Protein

Catalog #: 384-RG
2 Citations Datasheet / COA / SDS

Carrier Free

Catalog #	Availability	Size / Price	Qty
384-RG-050/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
384-RG-050

R&D Systems Recombinant Proteins and Enzymes

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All Common gamma Chain/IL-2 R gamma Proteins and Enzymes

Product Details

Citations (2)

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Common gamma Chain Protein Summary

Product Specifications

Purity

>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit the IL-2-dependent proliferation of MO7e human megakaryocytic leukemic cells. The ED₅₀ for this effect is typically 0.6-3.6 µg/mL in the presence of 30 µg/mL of soluble IL-2 R beta and 10 ng/mL of recombinant human IL-2.

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Common gamma Chain/IL-2 R gamma protein
Leu23-Asn254, with a C-terminal 6-His tag

Accession #

P31785

N-terminal Sequence
Analysis

Leu23

Predicted Molecular Mass

28.2 kDa

SDS-PAGE

40 kDa, reducing conditions

Product Datasheets

384-RG (with carrier)

Product Datasheet

COA

SDS

384-RG/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

384-RG

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 500 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

384-RG/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Common gamma Chain/IL-2 R gamma

The gamma chain of the high affinity functional human IL-2 receptor complex belongs to the hematopoietin receptor family. IL-2 R gamma is a 369 amino acid residue protein consisting of a 22 residue signal sequence, a 232 residue extracellular domain, a 29 residue transmembrane domain and an 86 residue cytoplasmic domain. Although IL-2 R gamma by itself does not bind IL-2 with any appreciable affinity, it is required for IL-2 receptor signaling. Besides IL-2, the gamma chain has been shown to be a component of the functional receptor complexes for IL-4, IL-7, IL-9 and IL-15. It has been proposed that IL-2 R gamma be designated the common gamma chain ( gamma _c). The site of molecular defects in X-linked SCID (severe combined immunodeficiency) has now been mapped to the IL-2 R gamma gene.

References

Minami, Y. et al. (1993) Annu. Rev. Immunol. 11:245.
Noguchi, M. et al. (1993) Science 262:1877.

Long Name

IL-2 Receptor gamma

Entrez Gene IDs

3561 (Human); 16186 (Mouse)

Alternate Names

CD132 antigen; CD132; CIDX; combined immunodeficiency, X-linked; common cytokine receptor gamma chain; Common gamma Chain; common gamma-chain; cytokine receptor common subunit gamma; gamma(c); IL-2 R gamma; IL-2 receptor subunit gamma; IL2R gamma; IL-2R subunit gamma; IL2RG; IL-2RG; IMD4; interleukin 2 receptor, gamma; Interleukin-2 receptor subunit gamma; P64; SCIDX; SCIDX1; severe combined immunodeficiency

Related Research Areas

Citations for Recombinant Human Common gamma Chain Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Highly potent anti-CD20-RLI immunocytokine targeting established human B lymphoma in SCID mouse.
Authors: Vincent M, Teppaz G, Lajoie L, Sole V, Bessard A, Maillasson M, Loisel S, Bechard D, Clemenceau B, Thibault G, Garrigue-Antar L, Jacques Y, Quemener A
MAbs, 2014-07-01;6(4):1026-37.
Applications: Bioassay
Identification and biochemical characterization of human plasma soluble IL-7R: lower concentrations in HIV-1-infected patients.
Authors: Rose T, Lambotte O, Pallier C, Delfraissy JF, Colle JH
J. Immunol., 2009-06-15;182(12):7389-97.
Applications: ELISA (Standard)