Recombinant Human Common gamma Chain Avi-tag His Protein, CF New
Recombinant Human Common gamma Chain Avi-tag His Protein, CF Summary
Product Specifications
Leu23-Asn254 with a C-terminal Avi-tag & 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
AVI384
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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Measured by its binding ability in a functional ELISA. Biotinylated Recombinant Human Common gamma Chain Avi-tag His-tag (Catalog # AVI384) binds to Recombinant Human CD25/IL-2R alpha Protein (223-2A), Recombinant Human IL-2R beta Protein (224-2B) and Recombinant Human IL-2 Protein (202-IL) with an ED50 of 0.100-1.00 μg/mL.
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2 μg/lane of Biotinylated Recombinant Human Common gamma Chain Avi-tag His-tag Protein (Catalog # AVI384) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 60‑67 kDa, under reducing conditions.
Reconstitution Calculator
Background: Common gamma Chain/IL-2 R gamma
The gamma chain of the high affinity functional human IL-2 receptor complex belongs to the hematopoietin receptor family. IL-2 R gamma is a 369 amino acid residue protein consisting of a 22 residue signal sequence, a 232 residue extracellular domain, a 29 residue transmembrane domain and an 86 residue cytoplasmic domain. Although IL-2 R gamma by itself does not bind IL-2 with any appreciable affinity, it is required for IL-2 receptor signaling. Besides IL-2, the gamma chain has been shown to be a component of the functional receptor complexes for IL-4, IL-7, IL-9 and IL-15. It has been proposed that IL-2 R gamma be designated the common gamma chain ( gamma c). The site of molecular defects in X-linked SCID (severe combined immunodeficiency) has now been mapped to the IL-2 R gamma gene. Our Avi-tag Biotinylated IL-2 R gamma features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.
- Minami, Y. et al. (1993) Annu. Rev. Immunol. 11:245.
- Noguchi, M. et al. (1993) Science 262:1877.
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