Recombinant Human Chitinase 3-like 2 Protein, CF
Recombinant Human Chitinase 3-like 2 Protein, CF Summary
Product Specifications
When 5 x 104 cells per well are added to rhCHI3L2 coated plates for 30 minutes at 37 °C, cell adhesion is enhanced in a dose dependent manner with typical ED50 range of 0.7-2.8 μg/mL.
Opitimal dilutions should be determined by each laboratory for each application.
Tyr27-Leu390, with a C-terminal 10-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
5112-CH
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Chitinase 3-like 2
Chitinase 3-like 2 (CHI3L2), also called chondrocyte protein 39 or YKL-39, is a 39 kDa potentially glycosylated member of the glycosyl hydrolase 18 family (1 - 3). It is secreted by synovial fibroblasts, chondrocytes, and TGF beta /IL-4-stimulated monocyte-derived macrophages (1, 4, 5). The human CHI3L2 cDNA encodes 390 amino acids (aa), including a 26 aa signal sequence and a 364 aa mature region. Potential alternate start sites at Met-24 and Met+80 would create isoforms of 413 aa and 311 aa, while a potential deletion of aa 15 - 24 would create a 380 aa isoform (6). Mature human CHI3L2 shares 89%, 87% and 84% aa sequence identity with bovine, porcine and equine CHI3L2, respectively. The rodent genome does not include a CHI3L2 ortholog (2). Human CHI3L1 and CHI3L2 share 43% aa sequence identity. Neither shows chitotriosidase activity, but both bind chitin and are thus termed chi-lectins (1 - 3). Unlike CHI3L1, CHI3L2 does not bind heparin (1). While CHI3L1 is the major protein excreted by chondrocytes, up-regulation of CHI3L2 and not CHI3L1 correlates with osteoarthritic cartilage degeneration (1, 7, 8). CHI3L2 has been proposed as a potential biomarker for osteoarthritis (4, 7, 8). CHI3L1 has been found to coordinate adhesion receptors and promote cell signaling and tumor angiogenesis; like CHI3L1, CHI3L2 can enhance cell adhesion (9).
- Hu, B. et al. (1996) J. Biol. Chem. 271:19415.
- Bussink, A.P. et al. (2007) Genetics 177:959.
- Renkema, G.H. et al. (1998) Eur. J. Biochem. 251:504.
- Gratchev, A. et al. (2008) Biomarker Insights 3:39.
- Benz, K. et al. (2002) Biochem. Biophys. Res. Commun. 293:284.
- Entrez Accession # EAW56480, NP_001020370, NP_001020368.
- Steck, E. et al. (2002) Biochem. Biophys. Res. Commun. 299:109.
- Knorr, T. et al. (2003) Ann. Rheum. Dis. 62:995.
- Shao, R. et al. (2009) Oncogene 28:4456.
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