Home / CEACAM-6/CD66c / Recombinant Human CEACAM-6/CD66c Protein, CF

Recombinant Human CEACAM-6/CD66c Protein, CF

Catalog #: 3934-CM
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
3934-CM-050
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CEACAM-6/CD66c Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of calcium ionophore treated human neutrophils. When 2 x 105 cells/well are added to CEACAM-6 coated plates (10 µg/mL, 100 µL/well), 35‑60% of the cells will adhere after 20 minutes at 37° C.
Source
Mouse myeloma cell line, NS0-derived human CEACAM-6/CD66c protein
Lys35-Gly320, with a C-terminal 6-His tag
Accession #
Q53XP7
N-terminal Sequence
Analysis
Lys35
Predicted Molecular Mass
32.0 kDa
SDS-PAGE
57-75 kDa, reducing conditions

Product Datasheets

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3934-CM

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3934-CM

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CEACAM-6/CD66c

Carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM-6), previously called nonspecific crossreacting antigen (NCA) or CD66c, is one of seven human CEACAM family members within the immunoglobulin superfamily (1 - 4). In humans, CEACAMs include type I transmembrane proteins (CEACAM-1, -3, and -4) and GPI-linked molecules (CEACAM-5 through -8) (1). There is no human CEACAM-2. Human CEACAM-6 is a 90 kDa, GPI-linked membrane protein that contains a 34 amino acid (aa) signal sequence, a 286 aa extracellular domain (ECD), and a 24 aa hydrophobic C-terminal propeptide. The GPI membrane anchor is attached at the C-terminus following cleavage of the propeptide. CEACAM-6 contains one N-terminal V-type Ig-like domain (N domain), followed by two C2-type Ig-like domains (2 - 4). It shows considerable glycosylation, including (sialyl) LewisX, which mediates binding to E-selectin, galectins and some bacterial fimbrae (1, 2). Mature human CEACAM-6 shows 84%, 85%, 80%, 87% and 51% aa identity to the equivalent extracellular regions of human CEACAMs 1, 5 (CEA) and 8, rhesus CEACAM-2, and bovine CEACAM-6, respectively. CEACAM-6 is expressed by granulocytes and their precursors. Activation enhances surface expression by mobilizing CEACAM-6 from storage in azurophilic granules (5, 6). It often shows aberrant expression in acute lymphocytic leukemias (10). CEACAM-6 is also expressed in epithelia of various organs and is upregulated in pancreatic and colon adenocarcinomas and hyperplastic polyps (7, 8). Over-expression confers resistance to adhesion-related apoptosis (anoikis) in tumor cells (8, 9). CEACAM-6 is an intercellular adhesion molecule, forming both homotypic, and heterotypic bonds with CEACAM-1, -5 and -8 through interaction of the V-type Ig-like domains (11, 12). Cross-linking of neutrophil CEACAM-6 augments alpha v beta 3 and beta 2 integrin-mediated adhesion, apparently by src and caveolin-mediated inside-out integrin activation(8, 13, 14).

References
  1. Beauchemin, N. et al. (1999) Exp. Cell Res. 252:243.
  2. Skubitz, K.M. et al. (1999) J. Biol. Regul. Homeost. Agents 13:244.
  3. Barnett, T. et al. (1988) Genomics 3:59.
  4. Tawaragi, Y. et al. (1988) Biochem. Biophys. Res. Comm. 150:89.
  5. Kuroki, M. et al. (1995) Immunol. Invest. 24:829.
  6. Ducker, T.P. and K.M. Skubitz (1992) J. Leukoc. Biol. 52:11.
  7. Scholzel, S. et al. (2000) Am. J. Pathol. 156:595.
  8. Duxbury, M.S. et al. (2004) J. Biol. Chem. 279:23176.
  9. Ilantzis, C. et al. (2002) Neoplasia 4:151.
  10. Kalina, T. et al. (2005) BMC Cancer 5:38.
  11. Oikawa, S. et al. (1992) Biochem. Biophys. Res. Commun. 186:881.
  12. Kuroki, M. et al. (2001) J. Leukoc. Biol. 70:543.
  13. Duxbury, M.S. et al. (2004) Biochem. Biophys. Res. Comm. 317:133.
  14. Skubitz, K.M. et al. (1999) J. Leukoc. Biol. 60:106.
Long Name
Carcinoembryonic Antigen-related Cell Adhesion Molecule 6
Entrez Gene IDs
4680 (Human); 107126399 (Cynomolgus Monkey)
Alternate Names
carcinoembryonic antigen-related cell adhesion molecule 6 (non-specific crossreacting antigen); carcinoembryonic antigen-related cell adhesion molecule 6; CD66c antigen; CD66c; CEACAM6; CEACAM-6; CEAL; NCA; NCACEAL; Non-specific crossreacting antigen; Normal cross-reacting antigen

Citations for Recombinant Human CEACAM-6/CD66c Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Overexpression of CEACAM6 activates Src-FAK signaling and inhibits anoikis, through homophilic interactions in lung adenocarcinomas.
    Authors: Kim E, Cha Y, Jeong S, Chang Y
    Transl Oncol, 2022-03-28;20(0):101402.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. The Immunomodulatory CEA Cell Adhesion Molecule 6 (CEACAM6/CD66c) Is a Protein Receptor for the Influenza a Virus
    Authors: SK Rahman, MA Ansari, P Gaur, I Ahmad, C Chakravart, DK Verma, A Sharma, S Chhibber, N Nehal, D Wirth, SK Lal
    Viruses, 2021-04-21;13(5):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Bioassay

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