Recombinant Human CEACAM-21 Fc Chimera Protein, CF Summary
Product Specifications
0.07-0.7 μg/mL.
| Human CEACAM-21 (Trp35-Gly240) Accession # NP_001091976 | IEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9870-CM
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: |
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Scientific Data
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When Recombinant Human Galectin-3 (Human Cell-expressed) (Catalog # 8259-GA) is coated at 1 µg/mL, 100 µL/well, Recombinant Human CEACAM-21 Fc Chimera (Catalog # 9870-CM) binds with an ED50 of 0.07-0.7 µg/mL.
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2 μg/lane of Recombinant Human CEACAM-21 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 62 - 72 kDa and 130 - 140 kDa, respectively.
Reconstitution Calculator
Background: CEACAM-21
Carcinoembryonic antigen-related cell adhesion molecule-21 (CEACAM-21) is a member of the CEACAM subfamily of glycoproteins in the immunoglobulin (Ig) superfamily. The CEACAM-21 gene codes for both the CEACAM-3 and CEACAM-21 proteins and is located on chromosome 19. For CEACAM-21, the gene codes a 258 amino acid (aa) polypeptide consisting of a 206 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 32 aa cytoplasmic domain. The mature ECD contains a single IgV-like domain, common to all members of the CEACAM family, and one IgC2-like domain (1). CEACAM-21 is thought to be a primate-specific gene, and to date, no orthologs have been identified in lower species (2). Originally discovered as a biomarker for colorectal cancer (3), CEACAMs have now been associated with numerous intracellular signaling processes including cell adhesion, cell growth, recognition and differentiation, angiogenesis, and apoptosis (4-6). Although the specific function of the CEACAM-21 protein has yet to be elucidated, CEACAM-21 has been found to be overexpressed in heavy smokers (7) and is associated with COPD susceptibility (8). It is also a candidate gene for schizophrenia in the Jewish population (9). CEACAM family members were identified as the major Galectin-3 receptor candidates on human neutrophils (10). Binding of carbohydrate ligands to CEACAMs may be important in the release of proinflammatory mediators (11, 12).
- Tchoupa, A. et al. (2014) J Cell Commun Signal 12:27.
- Zebhauser, R. et al. (2005) Genomics 86:566.
- Gold, P. and Freedman S.O. (1965) J Exp Med 122:467.
- Obrink, B. (1997) Curr Opin Cell Biol 9:616.
- Horst, A.K. Wagener, C. (2004) Handb Exp Pharmacol 283.
- Kuespert, K. et al. (2006) Curr Opin Cell Biol 18:565.
- Fukuda, I. et al. (1998) J. Med. Syst. 22:89.
- Qiu, W. et al. (2011) PLoS ONE 6:e24395.
- Alkelai, A. et al. (2012) Int. J. Neuropsycopharmacol. 15:459.
- Feuk-Lagerstedt, E. et al. (1999) J. Immunol. 163:5592.
- Yoon, J. et al. (2007) J. Immunol. 179:8454.
- Schröder, A.K. et al. (2006) Hum Immunol. 67:676.
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