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Recombinant Human CD94 Protein, CF

Catalog #: 9270-CD Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
9270-CD-050
Recombinant Human CD94 Protein Bioactivity
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CD94 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse NKG2A/CD159a Fc Chimera (Catalog # 9198-NK) is coated at 0.5 µg/mL, 100 μL/well, recombinant human CD94 binds with a typical ED50 of 0.1-0.6 μg/mL
Source
Chinese Hamster Ovary cell line, CHO-derived human CD94 protein
Lys32-Ile179, with an N-terminal 6-His tag
Accession #
Q13241
N-terminal Sequence
Analysis
Lys32
Predicted Molecular Mass
18 kDa
SDS-PAGE
21-38 kDa, reducing conditions

Product Datasheets

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9270-CD

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9270-CD

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Human CD94 Protein Bioactivity View Larger

When Recombinant Mouse NKG2A Fc Chimera (Catalog # 9198-NK) is coated at 0.5 µg/mL, Recombinant Human CD94 (Catalog # 9270-CD) binds with an ED50 of 0.1-0.6 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD94

CD94 is an approximately 25 kDa type 2 transmembrane protein that plays an important role in regulating natural killer (NK) cell activation (1). Human CD94 consists of a 10 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 148 aa extracellular domain (ECD) with a stem region and one C-type lectin domain (2). Alternative splicing generates additional isoforms that lack either the stem region, the terminal half of the ECD, or the cytoplasmic and transmembrane regions (3). CD94 is expressed at varying cell surface density on NK cells during their differentiation and on a subset of CD8+ T cells (4). It associates into
disulfide-linked heterodimers with NKG2A/B, C, or E (5-8), and these complexes function as receptors for the nonclassical MHC class I molecule, HLA-E (9, 10). Ligation of CD94-NKG2A or CD94-NKG2C on NK cells triggers inhibitory or activating signals, respectively (11).

References
  1. Chester, C. et al. (2015) Front. Immunol. 6:601.
  2. Chang, C. et al. (1995) Eur. J. Immunol. 25:2433.
  3. Lieto, L.D. et al. (2006) Genes Immun. 7:36.
  4. Yu, J. et al. (2010) Blood 115:274.
  5. Phillips, J.H. et al. (1996) Immunity 5:163.
  6. Lazetic, S. et al. (1996) J. Immunol. 157:4741.
  7. Brooks, A.G. et al. (1997) J. Exp. Med. 185:795.
  8. Carretero, M. et al. (1997) Eur. J. Immunol. 27:563.
  9. Braud, V.M. et al. (1998) Nature 391:795.
  10. Kraemer, T. et al. (2014) J. Immunol. Res. 2014:352160.
  11. Houchins, J.P. et al. (1997) J. Immunol. 158:3603.
Entrez Gene IDs
3824 (Human); 16643 (Mouse); 102119829 (Cynomolgus Monkey)
Alternate Names
CD94 antigen; CD94; CD94natural killer cells antigen CD94; Killer cell lectin-like receptor subfamily D member 1; killer cell lectin-like receptor subfamily D, member 1; KLRD1; KP43; NK cell receptor

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