Recombinant Human CD63 Fc Chimera Protein, CF

Recombinant Human CD63 Fc Chimera Protein, CF Summary

Product Specifications

Product Datasheets

Carrier Free

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: CD63

CD63, also known as Tspan-30, is a member of the tetraspanin family of proteins that mediate signal transduction events which play a role in the regulation of cell development, activation, growth and motility (1, 2). These membrane proteins are characterized by the presence of four transmembrane domains, a small extracellular loop (SEL) and a large extracellular loop (LEL) (2, 3). Regions of the tetraspanin LEL are known to associate with integrins to regulate membrane protein trafficking, leukocyte recruitment, and adhesion processes (4, 5). Deficiency of CD63 is associated with Hermansky-Pudlak syndrome (6). Human CD63-LEL shares 67% and 66% aa sequence identity with mouse and rat CD63-LEL, respectively.  CD63 has been identified as a cell surface binding partner for tissue inhibitor of metalloproteinase 1 (TIMP-1), modulating the tetraspanin/integrin signaling complex which regulates cell survival and polarization (7).

Related Research Areas

• Adhesion Molecules in Angiogenesis
• Alpha Granule Membrane Proteins
• Cancer Biomarkers
• Integrins and Associated Molecules
• Lysosomal Enzymes
• Platelet Markers