Recombinant Human CD47 Fc Chimera Protein, CF Summary
Product Specifications
Optimal dilutions should be determined by each laboratory for each application.
Also measured by its ability to antagonize red blood cell adhesion to immobilized rhSIRP alpha Fc Chimera. The ED50 for this effect is 0.5-2.0 μg/mL.| Human CD47 (Gln19-Pro139) Accession # Q08722 | IEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
4670-CD
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 200 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: CD47
CD47, also known as Integrin-Associated Protein (IAP) and OA3, is a 40-60 kDa variably glycosylated atypical member of the immunoglobulin superfamily (1, 2). Human CD47 is an integral membrane protein that consists of a 123 amino acid (aa) extracellular domain (ECD) with a single Ig-like domain, five membrane-spanning regions with short intervening loops, and a 34 aa C-terminal cytoplasmic tail (3). Alternate splicing of human CD47 generates additional isoforms with deletions in the cytoplasmic tail (3). Within the N-terminal ECD, human CD47 shares 63% aa sequence identity with mouse and rat CD47. A portion of the N‑terminal ECD can by shed from smooth muscle cells by MMP-2-mediated proteolysis (4). The ubiquitously expressed CD47 binds to SIRP family members on macrophages, neutrophils, and T cells (5, 6). These interactions prevent macrophage-mediated clearance of healthy CD47-expressing cells and promote immune cell transmigration across the vascular endothelium (5-8). The CD47-SIRP alpha interaction is species specific, and this lack of cross-species interaction has been implicated in xenotransplantation rejection (16). CD47 associates in cis with Fas on T cells and enhances Fas‑mediated apoptosis; its ligation promotes T cell anergy and dampens Th1 immune responses (9-11). CD47 also associates in cis with Integrins alpha 4 beta 1, alpha V beta 3, alpha 2b beta 3, and alpha 2 beta 1 which can positively or negatively modulate Integrin‑mediated function (2, 12). In the vasculature, CD47 binding by Thrombospondin-1 inhibits the angiogenic and vasorelaxant effects of nitric oxide (2, 13, 14). On dendritic cells and myeloma cells, CD47 ligation by TSP-1 induces giant cell formation and osteoclast differentiation (15).
- Sarfati, M. et al. (2009) Curr. Drug Targ. 9:842.
- Isenberg, J.S. et al. (2008) Arterioscler. Thromb. Vasc. Biol. 28:615.
- Campbell, I.G. et al. (1992) Cancer Res. 52:5416.
- Maile, L.A. et al. (2008) Mol. Endocrinol. 22:1226.
- Oldenborg, P.-A. et al. (2000) Science 288:2051.
- Liu, Y. et al. (2002) J. Biol. Chem. 277:10028.
- Stefanidakis, M. et al. (2008) Blood 112:1280.
- de Vries, H.E. et al. (2002) J. Immunol. 168:5832.
- Manna, P.P. et al. (2005) J. Biol. Chem. 280:29637.
- Avice, M.-N. et al. (2001) J. Immunol. 167:2459.
- Bouguermouh, S. et al. (2008) J. Immunol. 180:8073.
- Barazi, H.O. et al. (2002) J. Biol. Chem. 277:42859.
- Isenberg, J.S. et al. (2009) Nitric Oxide 21:52.
- Isenberg, J.S. et al. (2009) Matrix Biol. 28:110.
- Kukreja, A. et al. (2009) Blood 114:3413.
- Wang H. et al. (2007) Blood 109:836.
Citations for Recombinant Human CD47 Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Preclinical development of SGN-CD47M: Protease-activated antibody technology enables selective tumor targeting of the innate immune checkpoint receptor CD47
Authors: Levengood, MR;Carosino, CM;Zhang, X;Lucas, S;Ortiz, DJ;Westendorf, L;Chin, AP;Martin, AD;Wong, A;Hengel, SM;Sun, H;Zeng, W;Yumul, R;Dominguez, MM;Chen, Y;Zheng, JH;Karlsson, CAB;Trang, VH;Senter, PD;Gardai, SJ;
Molecular cancer therapeutics
Species: Human
Sample Types: Plasma, Antibody
Applications: ELISA Capture -
Comparative Analysis of piggyBac, CRISPR/Cas9 and TALEN Mediated BAC Transgenesis in the Zygote for the Generation of Humanized SIRPA Rats
Sci Rep, 2016-08-17;6(0):31455.
Species: Transgenic Rat
Sample Types: Whole Cells
Applications: Flow Cytometry -
Downregulation of CD47 and CD200 in patients with focal cortical dysplasia type IIb and tuberous sclerosis complex
Authors: FJ Sun, CQ Zhang, X Chen, YJ Wei, S Li, SY Liu, ZL Zang, JJ He, W Guo, H Yang
J Neuroinflammation, 2016-04-19;13(1):85.
Species: Human
Sample Types: Whole Tissue
Applications: Bioassay
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