Recombinant Human CD45RB Fc Chimera Protein, CF

Catalog #: 10477-CD Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10477-CD-050

Recombinant Human CD45RB Fc Chimera Protein Binding Activity

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Product Details

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CD45RB Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human Galectin-9 (Catalog # 2045-GA) is immoblized at 1 µg/mL (100 µL/well), the concentration of Recombinant Human CD45RB Fc Chimera (Catalog # 10477-CD) that produces a 50% optimal binding response is found to be 125-750 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human CD45RB protein

Human CD45RB (Gln26-Lys463) Accession # XP_006711537.1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

XP_006711537.1

N-terminal Sequence
Analysis

Gln26, inferred by deblocking revealing Ser25

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

75.6 kDa

SDS-PAGE

123-152 kDa, under reducing conditions

Product Datasheets

10477-CD

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10477-CD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human Galectin-9 (2045-GA) is immobilized at 1 µg/mL (100 µL/well), the concentration of Recombinant Human CD45RB Fc Chimera (Catalog # 10477-CD) that produces 50% optimal binding response is found to be 125‑750 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human CD45RB Fc Chimera Protein (Catalog # 10477-CD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 123-152 kDa and 250-300 kDa, respectively.

Reconstitution Calculator

Background: CD45RB

CD45, previously called LCA (leukocyte common antigen), T200, or Ly5 in mice, is member C of the class 1 (receptor-like) protein tyrosine phosphatase family (PTPRC) (1, 2). It is a variably glycosylated 180-220 kDa transmembrane protein that is abundantly expressed on all nucleated cells of hematopoietic origin (1-3). Multiple splicing isoforms of exon 4 (A), 5 (B), and 6 (C) are expressed according to cell type, developmental stage and antigenic exposure (1-5). The longest form, CD45RABC (called B220 in mouse) is expressed on B lymphocytes and the shortest form, CD45R0 is expressed on memory cells (5). Isoform CD45RB contains exon 5 (B exon) in the extracellular domain, which shares 41% and 40% homology with the ECD of mouse and rat CD45RB. CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds (3, 6-8). CD45 is moved into or out of the immunological synapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton (9, 10). Interaction of galaction can be fine-tuned by varying usage of the heavily O-glycosylated spliced regions and sialylation of N-linked carbohydrates of CD45 (4, 9). Within the immunological synapse, CD45 dephosphorylates and negatively regulates the Src family kinase, Lck (8-10). In other leukocytes, CD45 influences differentiation and links immunoreceptor signaling with cytokine secretion and cell survival, partially overlapping in function with DEP-1/CD148 (11-14). CD45 deletion causes in severe immunodeficiency, while point mutations may be associated with autoimmune disorders (6, 7).

References

Anderson, J.N. et al. (2004) FASEB J. 18:8.
Streuli, M. et al. (1987) J. Exp. Med. 166:1548.
Hermiston, M.L. et al. (2003) Annu. Rev. Immunol. 21:107.
Earl, L.A. and L.G. Baum (2008) Immunol. Cell Biol. 86:608.
Ralph, S.J. et al. (1987) EMBO J. 6:1251.
Falahti, R. and D. Leitenberg (2008) J. Immunol. 181:6082.
Tchilian, E.Z. and P.C.L. Beverley (2006) Trends Immunol. 27:146.
McNiell, L. et al. (2007) Immunity 27:425.
Chen, I-J. et al. (2007) J. Biol. Chem. 282:35361.
Freiberg, B.A. et al. (2002) Nat. Immunol. 3:911.
Zhu, J.W. et al. (2008) Immunity 28:183.
Huntington, N.D. et al. (2006) Nat. Immunol. 7:190.
Hesslein, D.G. et al. (2006) Proc. Natl. Acad. Sci. USA 103:7012.
Cross, J.L. et al. (2008) J. Immunol. 180:8020.

Long Name

CD45R, B isoform

Entrez Gene IDs

5788 (Human); 19264 (Mouse)

Alternate Names

B220; CD45 antigen; CD45R; CD45RB; EC 3.1.3.48; L-CA; LY5; protein tyrosine phosphatase, receptor type, C; receptor-type tyrosine-protein phosphatase C; T200 glycoprotein; T200 leukocyte common antigen; T200receptor type, c polypeptide