Recombinant Human CD44 Fc Chimera Protein, CF Summary
Product Specifications
| Human CD44 (Gln21-Pro220) Accession # P16070 |
IEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
3660-CD
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: CD44
CD44 is a ubiquitously expressed protein that is the major receptor for hyaluronan and exerts control over cell growth and migration (1-3). Human CD44 has a 20 amino acid (aa) signal sequence, an extracellular domain (ECD) with a 100 aa hyaluronan-binding disulfide-stabilized link region and a 325-530 aa stem region, a 21 aa transmembrane domain, and a 72 aa cytoplasmic domain. Within the stem, ten variably spliced exons (v1-10, exons 6-15) produce multiple protein isoforms (1-3). The standard or hematopoietic form, CD44H, does not include the variable segments (1-3). Cancer aggressiveness and T cell activation have been correlated with expression of specific isoforms (1, 3). With variable N- and O-glycosylation and splicing within the stalk, CD44 can range from 80 to 200 kDa (1). Within the N-terminal invariant portion of the ECD (aa 21-220), human CD44 shares 76%, 76%, 86%, 83% and 79% identity with corresponding mouse, rat, equine, canine and bovine CD44, respectively. The many reported functions of CD44 fall within three categories (1). First, CD44 binds hyaluronan and other ligands within the extracellular matrix and can function as a “platform” for growth factors and metalloproteinases. Second, CD44 can function as a co-receptor that modifies activity of receptors including MET and the ERBB family of tyrosine kinases. Third, the CD44 intracellular domain links the plasma membrane to the actin cytoskeleton via the ERM proteins, ezrin, radixin and moesin. CD44 can be synthesized in a soluble form (4) or may be cleaved at multiple sites by either membrane-type matrix metalloproteinases, or ADAM proteases to produce soluble ectodomains (5, 6). The cellular portion may then undergo gamma secretase-dependent intramembrane cleavage to form an A beta -like transmembrane portion and a cytoplasmic signaling portion that affects gene expression (7, 8). These cleavage events are thought to promote metastasis by enhancing tumor cell motility and growth (1, 5).
- Ponta, H. et al. (2003) Nat. Rev. Mol. Cell Biol. 4:33.
- Screaton, G.R. et al. (1992) Proc. Natl. Acad. Sci. USA 89:12160.
- Lynch, K.W. (2004) Nat. Rev. Immunol. 4:931.
- Yu, Q. and B.P. Toole (1996) J. Biol. Chem. 271:20603.
- Nagano, O. and H. Saya (2004) Cancer Sci. 95:930.
- Nakamura, H. et al. (2004) Cancer Res. 64:876.
- Murakami, D. et al. (2003) Oncogene 22:1511.
- Lammich, S. et al. (2002) J. Biol. Chem. 277:44754.
Citations for Recombinant Human CD44 Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
14
Citations: Showing 1 - 10
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Clinical Staphylococcus aureus inhibits human T-cell activity through interaction with the PD-1 receptor
Authors: Mellergaard, M;Skovbakke, SL;Jepsen, SD;Panagiotopoulou, N;Hansen, ABR;Tian, W;Lund, A;Høgh, RI;Møller, SH;Guérillot, R;Hayes, AS;Erikstrup, LT;Andresen, L;Peleg, AY;Larsen, AR;Stinear, TP;Handberg, A;Erikstrup, C;Howden, BP;Goletz, S;Frees, D;Skov, S;
mBio
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry Control -
Advanced bioactive glue tethering Lubricin/PRG4 to promote integrated healing of avascular meniscus tears
Authors: Tarafder, S;Ghataure, J;Langford, D;Brooke, R;Kim, R;Eyen, SL;Bensadoun, J;Felix, JT;Cook, JL;Lee, CH;
Bioactive materials
Species: Human
Sample Types: Whole Tissue
Applications: IHC, Bioassay -
CD44 acts as a coreceptor for cell-specific enhancement of signaling and regulatory T cell induction by TGM1, a parasite TGF-? mimic
Authors: van Dinther, M;Cunningham, KT;Singh, SP;White, MPJ;Campion, T;Ciancia, C;van Veelen, PA;de Ru, AH;González-Prieto, R;Mukundan, A;Byeon, CH;Staggers, SR;Hinck, CS;Hinck, AP;Dijke, PT;Maizels, RM;
Proceedings of the National Academy of Sciences of the United States of America
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Highly sensitive quantification of bacterial chondroitin in a culture based on ELISA techniques
Authors: K Suzuki, H Kaseyama-T, S Ito
Journal of microbiological methods, 2022-09-16;0(0):106579.
Species: Bacteria - Escherichia coli
Sample Types: Protein
Applications: Surface Plasmon Resonance (SPR -
Oxidative damage to hyaluronan-CD44 interactions as an underlying mechanism of action of oxidative stress-inducing cancer therapy
Authors: M Yusupov, A Privat-Mal, RM Cordeiro, H Verswyvel, P Shaw, J Razzokov, E Smits, A Bogaerts
Redox Biology, 2021-04-11;43(0):101968.
Species: Human
Sample Types: Protein
Applications: Bioassay -
Identification of novel CD44v6-binding peptides that block CD44v6 and deliver a pro-apoptotic peptide to tumors to inhibit tumor growth and metastasis in mice
Authors: F Khan, S Gurung, GR Gunassekar, SMP Vadevoo, L Chi, U Permpoon, ME Haque, YK Lee, SW Lee, S Kim, B Lee
Theranostics, 2021-01-01;11(3):1326-1344.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay -
Proteoglycan serglycin promotes non-small cell lung cancer cell migration through the interaction of its glycosaminoglycans with CD44
Authors: JY Guo, CH Chiu, MJ Wang, FA Li, JY Chen
J. Biomed. Sci., 2020-01-02;27(1):2.
Species: Human
Sample Types:
Applications: Cell Culture -
An innovative immunotherapeutic strategy for ovarian cancer: CLEC10A and glycomimetic peptides
Authors: LL Eggink, KF Roby, R Cote, J Kenneth Ho
J Immunother Cancer, 2018-04-17;6(1):28.
Species: Human
Sample Types: Peptide
Applications: Binding Assay -
Role of CD44 in Regulating TLR2 Activation of Human Macrophages and Downstream Expression of Proinflammatory Cytokines
Authors: M Qadri, S Almadani, GD Jay, KA Elsaid
J. Immunol., 2017-12-01;0(0):.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay -
Soluble Siglec-5 associates to PSGL-1 and displays anti-inflammatory activity
Sci Rep, 2016-11-28;6(0):37953.
Applications: Bioassay -
CD44 Binding to Hyaluronic Acid Is Redox Regulated by a Labile Disulfide Bond in the Hyaluronic Acid Binding Site.
Authors: Kellett-Clarke H, Stegmann M, Barclay A, Metcalfe C
PLoS ONE, 2015-09-17;10(9):e0138137.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay -
Langerhans Cell-Dendritic Cell Cross-Talk via Langerin and Hyaluronic Acid Mediates Antigen Transfer and Cross-Presentation of HIV-1.
Authors: van den Berg L, Cardinaud S, van der Aar A, Sprokholt J, de Jong M, Zijlstra-Willems E, Moris A, Geijtenbeek T
J Immunol, 2015-07-13;195(4):1763-73.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Broad spectrum activity of a lectin-like bacterial serine protease family on human leukocytes.
Authors: Ayala-Lujan, Jorge Lu, Vijayakumar, Vidhya, Gong, Mei, Smith, Rachel, Santiago, Araceli, Ruiz-Perez, Fernando
PLoS ONE, 2014-09-24;9(9):e107920.
Species: Bacteria, Human
Sample Types: Protein, Whole Cells
Applications: Enzyme Assay, Enzyme Assay Substrate -
Anticancer chemotherapy inhibits MHC class I-related chain a ectodomain shedding by downregulating ADAM10 expression in hepatocellular carcinoma.
Authors: Kohga K, Takehara T, Tatsumi T, Miyagi T, Ishida H, Ohkawa K, Kanto T, Hiramatsu N, Hayashi N
Cancer Res., 2009-10-13;69(20):8050-7.
Species: Human
Sample Types: Whole Tissue
Applications: Bioassay
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MAB7045 was used as the capture antibody along with BBA10 as the detection antibody. Recombinant human CD44 Fc Chimera (3660-CD) was used as the calibrator material. Human serum and plasma samples were diluted 1:5 and all were quantifiable. Parallelism looked good.
