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Recombinant Human CD40 Ligand/TNFSF5 (Homotrimer)

Catalog #: 617-CL
10 Citations Datasheet / COA / SDS

Discontinued Product

617-CL has been discontinued.
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Citations (10)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CD40 Ligand/TNFSF5 (Homotrimer) Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using B cell-enriched peripheral blood mononuclear cells (PBMC) in the presence of IL-4. Spriggs, M.K. et al. (1992) J. Exp. Med. 176:1543. The ED50 for this effect is 1‑3 µg/mL in the presence of 20 ng/mL of
rhIL-4.
Source
E. coli-derived human CD40 Ligand/TNFSF5 protein
Glu108-Leu261, with an N-terminal Met
Accession # P29965
Accession #
P29965
N-terminal Sequence
Analysis
Met
Structure / Form
Non-covalently linked trimer
Predicted Molecular Mass
16.9 kDa (monomer)
SDS-PAGE
18 kDa, reducing conditions

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617-CL (with carrier)

Product Datasheet
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617-CL/CF (carrier free)

Discontinued Product

617-CL

Formulation Lyophilized from a 0.2 μm filtered solution in NaH2PO4, NaCl and EDTA with BSA as a carrier protein.
Reconstitution Reconstitute at 500 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: CD40 Ligand/TNFSF5

CD40 ligand, CD40L (also known as CD154, TRAP or gp39), is a 261 amino acid type II transmembrane glycoprotein belonging to the TNF family, CD40L is expressed predominantly on activated CD4+ T lymphocytes, and also found in other types of cells, like NK cells, mast cells, basophils and eosinophils. Human CD40L shares 78% amino acid identity with its murine counterpart. The receptor of CD40L is CD40, a type I transmembrane glycoprotein belonging to the TNF receptor family. CD40 is expressed on B lymphocytes, monocytes, dendritic cells and thymic epithelium. Although all monomeric, dimeric and trimeric forms of soluble CD40L can bind to CD40, the trimeric form of soluble CD40L has the most potent biological activity through oligomerization of cell surface CD40, a common feature of TNF receptor family members. CD40L mediates a range of activities on B cells including induction of activation-associated surface antigen, entry into cell cycle, isotype switching and Ig secretion and memory generation. CD40-CD40L interaction also plays important roles in monocyte activation and dendritic cell maturation.

References
  1. Armitage, R.J. et al. (1992) Nature 357:80.
  2. Hollenbaugh, D. et al. (1992) EMBO J. 11:4313.
  3. Spriggs, M.K. et al. (1992) J. Exp. Med. 176:1543.
  4. Fanslow, W.C. et al. (1994) Seminars in Immunology 6:267.
  5. Kooten, C.V. and J. Banchereau (2000) J. Leukoc. Biol. 67:2.
Entrez Gene IDs
959 (Human); 21947 (Mouse); 84349 (Rat)
Alternate Names
CD154 antigen; CD154; CD40 antigen ligand; CD40 Ligand; CD40L; CD40-L; CD40LG; CD40LIGM; gp39; hCD40L; HIGM1; T-B cell-activating molecule; T-BAM; T-cell antigen Gp39; TNF-related activation protein; TNFSF5; TNFSF5IMD3; TRAP; TRAPtumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome); tumor necrosis factor (ligand) superfamily member 5; Tumor necrosis factor ligand superfamily member 5

Citations for Recombinant Human CD40 Ligand/TNFSF5 (Homotrimer)

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Zinc-finger protein 91 plays a key role in LIGHT-induced activation of non-canonical NF-kappaB pathway.
    Authors: Jin HR, Jin X, Lee JJ
    Biochem. Biophys. Res. Commun., 2010-09-06;400(4):581-6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Expression of soluble vascular endothelial growth factor receptor-1 in human monocyte-derived mature dendritic cells contributes to their antiangiogenic property.
    Authors: Kishuku M, Nishioka Y, Abe S, Kishi J, Ogino H, Aono Y, Azuma M, Kinoshita K, Batmunkh R, Rentsenhand B, Makino H, Ranjan P, Minakuchi K, Sone S
    J. Immunol., 2009-12-15;183(12):8176-85.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Interleukin-24 inhibits the plasma cell differentiation program in human germinal center B cells.
    Authors: Maarof G, Bouchet-Delbos L, Gary-Gouy H, Durand-Gasselin I, Krzysiek R, Dalloul A
    Blood, 2009-11-24;115(9):1718-26.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Soluble CD40 ligand as a biomarker for storage-related preanalytic variations of human serum.
    Authors: Lengelle J, Panopoulos E, Betsou F
    Cytokine, 2008-10-11;44(2):275-82.
    Species: Human
    Sample Types: Serum
    Applications: Bioassay
  5. Maturing dendritic cells are an important source of IL-29 and IL-20 that may cooperatively increase the innate immunity of keratinocytes.
    Authors: Wolk K, Witte K, Witte E, Proesch S, Schulze-Tanzil G, Nasilowska K, Thilo J, Asadullah K, Sterry W, Volk HD, Sabat R
    J. Leukoc. Biol., 2008-02-15;83(5):1181-93.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Unique cell surface expression of receptor tyrosine kinase ROR1 in human B-cell chronic lymphocytic leukemia.
    Authors: Baskar S, Kwong KY, Hofer T, Levy JM, Kennedy MG, Lee E, Staudt LM, Wilson WH, Wiestner A, Rader C
    Clin. Cancer Res., 2008-01-15;14(2):396-404.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Decreased expression of tumor necrosis factor family receptors involved in humoral immune responses in preterm neonates.
    Authors: Kaur K, Chowdhury S, Greenspan NS, Schreiber JR
    Blood, 2007-07-18;110(8):2948-54.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Human dendritic cells acquire a semimature phenotype and lymph node homing potential through interaction with CD4+CD25+ regulatory T cells.
    Authors: Bayry J, Triebel F, Kaveri SV, Tough DF
    J. Immunol., 2007-04-01;178(7):4184-93.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. A soluble BAFF antagonist, BR3-Fc, decreases peripheral blood B cells and lymphoid tissue marginal zone and follicular B cells in cynomolgus monkeys.
    Authors: Vugmeyster Y, Seshasayee D, Chang W, Storn A, Howell K, Sa S, Nelson T, Martin F, Grewal I, Gilkerson E, Wu B, Thompson J, Ehrenfels BN, Ren S, Song A, Gelzleichter TR, Danilenko DM
    Am. J. Pathol., 2006-02-01;168(2):476-89.
    Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Effect of piceatannol in human monocyte-derived dendritic cells in vitro.
    Authors: Takei M, Umeyama A, Arihara S, Matsumoto H
    J Pharm Sci, 2005-05-01;94(5):974-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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