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Recombinant Human CD277/BTN3A1 Fc Chimera Protein, CF

Catalog #: 8539-BT Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
8539-BT-050
Recombinant Human CD277/BTN3A1 Fc Chimera Protein Bioactivity
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CD277/BTN3A1 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE with silver staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is typically 1-5 µg/mL.
Source
Human embryonic kidney cell, HEK293-derived human CD277/BTN3A1 protein
Human CD277/BTN3A1
(Phe31-Gly254)
Accession # O00481		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
O00481
N-terminal Sequence
Analysis
Phe31
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
51 kDa
SDS-PAGE
54-62 kDa, reducing conditions

Product Datasheets

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8539-BT

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8539-BT

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Human CD277/BTN3A1 Fc Chimera Protein Bioactivity View Larger

Recombinant Human CD277/BTN3A inhibits anti-CD3 antibody-induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is 1-5 µg/mL.

SDS-PAGE Recombinant Human CD277/BTN3A1 Fc Chimera Protein SDS-PAGE View Larger

1 μg/lane of Recombinant Human CD277/BTN3A1 (Catalog # 8539-BT) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 58.8 and 150 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD277/BTN3A1

Butyrophilin 3A1 (also called BTN3A1) is a 57 kDa type I transmembrane glycoprotein member of the Ig superfamily. It is expressed on a wide variety of immune cells. Similar to BTN3A2 and BTN3A3, BTN3A1 (484 amino acids) is composed of an extracellular N-terminal IgV and a membrane-proximal IgC domain followed by a transmembrane domain and a cytoplasmic tail. These Ig domains are also found in B7 family co-stimulatory molecules, suggesting structural and functional similarities between the two protein families (1). The intracellular portion of BTN3A1 contains a B30.2 domain (2). Although the B30.2 domain of BTN1A1 binds to xanthine oxidoreductase (XOR) and is conserved among BTN1A1 orthologs, this interaction with XOR is not shared by BTN3A1 (3). The B30.2 domain of butyrophilins also functions as a sensor for detecting changes in intracellular phopho-antigen (pAg) concentrations produced during tumorigenesis and microbial infections (4, 5). The specific binding of pAg by the B30.2 domain of BTN3A1 induces a conformational change in its ECD, leading to the activation of V gamma 9Vδ2 T cells (6). Thus, BTN3A1 acts as a critical protein for the activation of V gamma 9Vδ2 T cells following detection of distressed cells (7). The anti-tumor responses of V gamma 9Vδ2 T cells may be enhanced with agonistic anti-BTNA3 antibodies (8). No BTN3A1 homolog has yet been identified in rodents. However, Human BTN3A1 shares 92.4% and 91.6% sequence identity with baboon and rhesus monkey BTN3A1 respectively (9).

References
  1. Abeler-Dorner, L. et al. (2012) Trends Immunol. 33:34.
  2. Rhodes, D.A. et al. (2001) Genomics 71:351.
  3. Jeong, J. et al. (2009) J. Biol. Chem. 284:22444.
  4. Harly, C. et al. (2012) Blood 120:2269.
  5. Constant, P. et al. (1994) Science 264:267.
  6. Sandstrom, A. et al. (2014) Immunity 40:490.
  7. Harly, C. et al. (2015) Front. Immunol. 5:657.
  8. Bonneville, M. et al. (2006) Curr. Opin. Immunol. 18:539.
  9. Wang, H. et al. (2013) J.Immunol. 191:1029.
Entrez Gene IDs
11119 (Human)
Alternate Names
BT3.1; BTF5; BTN3A1; butyrophilin, subfamily 3, member A1; CD277; dJ45P21.3 (butyrophilin, subfamily 3, member A1)

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