Recombinant Human CD25/IL-2R alpha Protein
Recombinant Human CD25/IL-2R alpha Protein Summary
Product Specifications
Glu22-Cys213
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
223-2A
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
223-2A/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 50 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: CD25/IL-2R alpha
Human IL-2 receptor alpha (IL-2 R alpha ), also known as Tac antigen and as CD25, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL‑2. The IL-2 R alpha cDNA encodes a 272 amino acid residue precursor Type I membrane protein with a 21 residue signal peptide, a 219 residue extracellular region, a 19 residue transmembrane region and a 13 residue cytoplasmic domain. IL-2 R alpha lacks structural features characteristic of members of the cytokine receptor superfamily. By itself, IL-2 R alpha binds IL-2 with low affinity. However, when IL-2 R alpha is associated with the IL-2 receptor beta and gamma chains, a high affinity heterotrimeric receptor complex that transduces IL-2 signals is formed.
Soluble forms of many cytokine receptors have been reported, and a soluble form of IL-2 R alpha (IL‑2 sR alpha ) appears in serum, concomitant with its increased expression on cells. The function of the soluble IL-2 R alpha is unclear. Increased levels of IL‑2 sR alpha in biological fluids reportedly correlate with increased T and B cell activation and immune system activation. Increased serum concentration of IL-2 sR alpha has been observed in patients with a variety of inflammatory conditions and in the course of some leukemias and lymphomas.
Citations for Recombinant Human CD25/IL-2R alpha Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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An IL-2-grafted antibody immunotherapy with potent efficacy against metastatic cancer
Authors: D Sahin, N Arenas-Ram, M Rath, U Karakus, M Hümbelin, M van Gogh, L Borsig, O Boyman
Nature Communications, 2020-12-22;11(1):6440.
Species: Human
Sample Types: Recombinant Protein
Applications: ELISA Capture -
The CD25-binding antibody Daclizumab High-Yield Process has a distinct glycosylation pattern and reduced antibody-dependent cell-mediated cytotoxicity in comparison to Zenapax�
MAbs, 2016-07-01;0(0):0.
Applications: Bioassay -
Imbalance in the peritoneal levels of interleukin 1 and its decoy inhibitory receptor type II in endometriosis women with infertility and pelvic pain.
Authors: Akoum A, Al-Akoum M, Lemay A, Maheux R, Leboeuf M
Fertil. Steril., 2007-10-24;89(6):1618-24.
Applications: ELISA (Standard)
FAQs
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What are the differences between Recombinant Human CD25/IL-2R alpha Protein (Catalog # 223-2A) and Recombinant Human CD25/IL-2R alpha His-tag Protein, CF (Catalog # 10305-RL)?
Catalog # 223-2A is expressed using the Mouse myeloma cell line, NS0, and is untagged.
Catalog # 10305-RL is expressed using the Human embryonic kidney cell line, HEK293, and contains a C-terminal 6-His tag.
The activity of these proteins is measured by their ability to inhibit the IL-2-dependent proliferation of MO7e human megakaryocytic leukemic cells. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. Catalog # 223-2A has an ED50 of 0.15‑0.75 µg/mL in the presence of 30 ng/mL of recombinant human IL-2, while Catalog # 10305-RL has an ED50 of 0.15-1.2 µg/mL in the presence of 30 ng/mL of Recombinant Human IL-2.
Reviews for Recombinant Human CD25/IL-2R alpha Protein
Average Rating: 5 (Based on 2 Reviews)
Have you used Recombinant Human CD25/IL-2R alpha Protein?
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Reason for Rating: This recombinant IL-2Ra worked very well as a calibrator in a sandwich ELISA. Human serum concentrations of IL-2Ra were calculated based of this calibration curve.
