Recombinant Human CD151 Fc Chimera Protein, CF Summary
Product Specifications
| MD | Human IgG1 (Pro100-Lys330) | IEGR | Human CD151-LEL (Ala113-Arg221) Accession # P48509 |
| N-terminus | C-terminus | ||
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1884-CD
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: |
|
Scientific Data
View Larger
When Recombinant Human Integrin aV beta 3 (Catalog # 3050-AV) is immobilized at 1 µg/mL, 100 µL/well, Recombinant Human CD151 Fc Chimera binds with an ED50 of 0.3-1.8 µg/mL.
View Larger
2 μg/lane of Recombinant Human CD11b/Integrin alpha M was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 41-55 kDa and 80 - 110 kDa, respectively.
Reconstitution Calculator
Background: CD151
Human CD151, also known as SFA-1, Tetraspanin-24, and GP27, is a palmitoylated glycoprotein in the tetraspanin superfamily. It is the first tetraspanin member to be identified as a promoter of cancer metastasis (1, 2), and it is found to participate in nearly all stages of cancer progression (3). CD-151 is normally expressed in endothelial cells, platelets, and frequently over-expressed in cancer cells (4). Mature CD-151 is a multi-pass membrane protein that contains four transmembrane domains, three cytoplasmic domains, and two extracellular loops. The region of amino acids (aa) 113-221 contains the largest extracellular loop (LEL) that involves in interacting with integrins and regulating integrin functions (5). Human CD151 LEL shares 88.9% and 87.0% aa identity with that of mouse and rat respectively. CD151 interacts with integrins such as alpha v beta 3, alpha 3 beta 1, alpha 6 beta 1, alpha 7 beta 1, and alpha 6 beta 4 to regulate their activities and thus resulting in modulation of adhesion, spreading, migration, angiogenesis, invasion and metastasis (1, 3-5). CD151 can also complex with immunoglobulin super family proteins and other tetraspanins such as CD9, CD81, and CD63 (3). Clinically, high levels of CD151 are correlated with poor prognosis in a variety of tumors (3, 4). CD151 has been implicated as a potential diagnostic marker in osteosarcoma and prostate cancer and a putative target for antibody-based immunotherapy (3). CD-151 is a key player in the formation of basement membranes in kidney and skin tissues; it is also associated with human papillomavirus (HPV) infection (6, 7).
- Detchokul, S. et al. (2014) Br. J. Pharmacol. 171(24):5462.
- Hasegawa, H. et al. (1996). Journal of Virology 70: 3258.
- Sadej, R. et al. (2014) Laboratory Investigation 94:41.
- Kumari, S. et al. (2015) Biomark Cancer 7:7.
- Yu J. et al. (2017) Biochem. J. 474(4):589.
- Scheffer, K.D. et al. (2014) Viruses 6:893.
- Karamatic Crew, V. et al. (2004) Blood 104:2217.
FAQs
No product specific FAQs exist for this product, however you may
View all Proteins and Enzyme FAQsReviews for Recombinant Human CD151 Fc Chimera Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Human CD151 Fc Chimera Protein, CF and earn rewards!
Have you used Recombinant Human CD151 Fc Chimera Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
