Recombinant Human CCL4/MIP-1 beta Protein

Name: Recombinant Human CCL4/MIP-1 beta Protein
Brand: R&D Systems
SKU: 271-BME
Rating: 5 (2 reviews)

Catalog #: 271-BME
26 Citations 2 Reviews Datasheet / COA / SDS

Carrier Free

Catalog #	Availability	Size / Price	Qty
271-BME-010/CF
271-BME-050/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
271-BME-010
271-BME-050

R&D Systems Recombinant Proteins and Enzymes

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All CCL4/MIP-1 beta Proteins and Enzymes

Product Details

Citations (26)

FAQs

Reviews (2)

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CCL4/MIP-1 beta Protein Summary

Product Specifications

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR5. The ED₅₀ for this effect is 1‑6 ng/mL.

Source

E. coli-derived human CCL4/MIP-1 beta protein
Ala24-Asn92 (major); Pro30-Asn92 (Minor)

Accession #

P13236.1

N-terminal Sequence
Analysis

Ala24 (Major); Pro30 (Minor)

Predicted Molecular Mass

7.8 kDa & 7.3 kDa

Product Datasheets

271-BME (with carrier)

Product Datasheet

COA

SDS

271-BME/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

271-BME

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

271-BME/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: CCL4/MIP-1 beta

CCL4, also known as macrophage inflammatory protein 1 beta (MIP-1 beta ) is a 7.8 kDa beta chemokine that is secreted at sites of inflammation by activated leukocytes, lymphocytes, vascular endothelial cells, and pulmonary smooth muscle cells (1, 2). CCL4 attracts a variety of immune cells to sites of microbial infection as well as to other pathologic inflammation such as allergic asthma and ischemic myocardium (3 - 8). A CCL4 deficiency in mice promotes the development of autoantibodies, possibly as a result of compromised regulatory T cell recruitment (6). CCL4 is secreted from activated monocytes as a heterodimer with CCL3/MIP-1 alpha (9). The first two N-terminal amino acids can be cleaved from human CCL4 by CD26/DPPIV (10, 11). Both the full length and truncated forms exert biological activity through CCR5, and the truncated form additionally interacts with CCR1 and CCR2b (10). In humans, the ability of CCL4 to bind CCR5 inhibits the cellular entry of M-tropic HIV-1 which utilizes CCR5 as a coreceptor (2). Both forms of CCL4 block HIV-1 infection of T cells by inducing the downregulation of CCR5 (10). Mature human CCL4 shares 77% and 80% aa sequence identity with mouse and rat CCL4, respectively.

References

Rot, A. and U.H. von Andrian (2004) Annu. Rev. Immunol. 22:891.
Menten, P. et al. (2002) Cytokine Growth Factor Rev. 13:455.
Sun, X. et al. (2006) Infec. Immun. 74:5943.
Bisset, L.R. and Schmid-Grendelmeier, P. (2005) Curr. Opin. Pulm. Med. 11:35.
Frangogiannis, N.G. (2004) Inflamm. Res. 53:585.
Bystry, R.S. et al. (2001) Nat. Immunol. 2:1126.
Oliveira, S.H.P. et al. (2002) J. Leukoc. Biol. 71:1019.
Schall, T.J. et al. (1993) J. Exp. Med. 177:1821.
Guan, E. et al. (2001) J. Biol. Chem. 276:12404.
Guan, E. et al. (2002) J. Biol. Chem. 277:32348.
Guan, E. et al. (2004) J. Cell. Biochem. 92:53.

Entrez Gene IDs

6351 (Human); 20303 (Mouse); 116637 (Rat); 448786 (Canine); 102117861 (Cynomolgus Monkey)

Alternate Names

ACT2; ACT-2; AT744.1; C-C motif chemokine 4; CCL4; chemokine (C-C motif) ligand 4; Exodus-3; G-26 T-lymphocyte-secreted protein; G-26; HC21; LAG1; LAG-1; Lymphocyte activation gene 1 protein; Macrophage inflammatory protein 1-beta; MIP1 beta; MIP-1 beta; MIP1B; MIP1B1; MIP-1-beta; MIP-1-beta(1-69); PAT 744; Protein H400; SCYA2; SCYA4; secreted protein G-26; SIS-gamma; small inducible cytokine A4 (homologous to mouse Mip-1b); Small-inducible cytokine A4; T-cell activation protein 2

Related Research Areas

Citations for Recombinant Human CCL4/MIP-1 beta Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

26 Citations: Showing 1 - 10
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A keratinocyte-adipocyte signaling loop is reprogrammed by loss of BTG3 to augment skin carcinogenesis
Authors: Cheng, YC;Acedera, JD;Li, YJ;Shieh, SY;
Cell death and differentiation
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Role of CCR3 in respiratory syncytial virus infection of airway epithelial cells.
Authors: Wellemans V, Benhassou H, Fuselier E, Bellesort F, Dury S, Lebargy F, Dormoy V, Fichel C, Naour R, Gounni A, Lamkhioued B
iScience, 2021-11-14;24(12):103433.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Transmigration across a Steady-State Blood-Brain Barrie Induces Activation of Circulating Dendritic Cells Partly Mediated by Actin Cytoskeletal Reorganization
Authors: M Meena, M Van Delen, M De Laere, A Sterkens, C Costas Rom, Z Berneman, N Cools
Membranes, 2021-09-13;11(9):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Proinflammatory oscillations over the menstrual cycle drives bystander CD4 T cell recruitment and SHIV susceptibility from vaginal challenge
Authors: A Swaims-Koh, AN Sheth, J Brody, FP Hardnett, S Sharma, EW Bonning, I Ofotokun, I Massud, JG García-Ler
EBioMedicine, 2021-07-03;69(0):103472.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Human NK cells adapt their immune response towards increasing multiplicities of infection of Aspergillus fumigatus
Authors: L Marischen, A Englert, AL Schmitt, H Einsele, J Loeffler
BMC Immunol., 2018-12-18;19(1):39.
Applications: Western Blot
Adsorption as a Contributor for Inflammatory Mediators Removal by Different Hemofiltration Membranes: A Pilot Study
Authors: Benjamin Malard
Artif Organs, 2016-12-02;0(0):.
Species: Human
Sample Types: Plasma
Applications: Hemofiltration Procedure
HIV-1 gp120 activates the STAT3/interleukin-6 axis in primary human monocyte-derived dendritic cells.
Authors: Del Corno M, Donninelli G, Varano B, Da Sacco L, Masotti A, Gessani S
J Virol, 2014-07-09;88(19):11045-55.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
CCR5 is a receptor for Staphylococcus aureus leukotoxin ED.
Authors: Alonzo, Francis, Kozhaya, Lina, Rawlings, Stephen, Reyes-Robles, Tamara, DuMont, Ashley L, Myszka, David G, Landau, Nathanie, Unutmaz, Derya, Torres, Victor J
Nature, 2012-12-12;493(7430):51-5.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
Nature, 2012-07-26;487(7408):505-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Thymoquinone inhibits the CXCL12-induced chemotaxis of multiple myeloma cells and increases their susceptibility to Fas-mediated apoptosis.
Authors: Badr G, Lefevre EA, Mohany M
PLoS ONE, 2011-09-01;6(9):e23741.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

CCL19 is a specific ligand of the constitutively recycling atypical human chemokine receptor CRAM-B.
Authors: Leick M, Catusse J, Follo M, Nibbs RJ, Hartmann TN, Veelken H, Burger M
Immunology, 2009-12-02;129(4):536-46.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases.
Authors: Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D
J. Immunol., 2008-12-01;181(11):8053-67.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

C-reactive protein (CRP) induces chemokine secretion via CD11b/ICAM-1 interaction in human adherent monocytes.
Authors: Montecucco F, Steffens S, Burger F, Pelli G, Monaco C, Mach F
J. Leukoc. Biol., 2008-06-30;84(4):1109-19.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Regulation of D6 chemokine scavenging activity by ligand- and Rab11-dependent surface up-regulation.
Authors: Bonecchi R, Borroni EM, Anselmo A, Doni A, Savino B, Mirolo M, Fabbri M, Jala VR, Haribabu B, Mantovani A, Locati M
Blood, 2008-05-14;112(3):493-503.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Expression of CCR5 receptors on Reed-Sternberg cells and Hodgkin lymphoma cell lines: involvement of CCL5/Rantes in tumor cell growth and microenvironmental interactions.
Authors: Aldinucci D, Lorenzon D, Cattaruzza L, Pinto A, Gloghini A, Carbone A, Colombatti A
Int. J. Cancer, 2008-02-15;122(4):769-76.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Cognate CD4+ T-cell-dendritic cell interactions induce migration of immature dendritic cells through dissolution of their podosomes.
Authors: Nobile C, Lind M, Miro F, Chemin K, Tourret M, Occhipinti G, Dogniaux S, Amigorena S, Hivroz C
Blood, 2008-01-18;111(7):3579-90.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Human dendritic cells acquire a semimature phenotype and lymph node homing potential through interaction with CD4+CD25+ regulatory T cells.
Authors: Bayry J, Triebel F, Kaveri SV, Tough DF
J. Immunol., 2007-04-01;178(7):4184-93.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
Blood, 2007-02-08;109(10):4272-9.
Applications: ELISA (Standard)

Inhibition of HIV-1 infection by viral chemokine U83A via high-affinity CCR5 interactions that block human chemokine-induced leukocyte chemotaxis and receptor internalization.
Authors: Catusse J, Parry CM, Dewin DR, Gompels UA
Blood, 2007-01-05;109(9):3633-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1.
Authors: Grivel JC, Elliott J, Lisco A, Biancotto A, Condack C, Shattock RJ, McGowan I, Margolis L, Anton P
AIDS, 2007;21(10):1263-72.
Species: N/A
Sample Types: N/A
Applications: ELISA (Standard)

Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
Authors: Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B
Clin. Immunol., 2006-06-16;120(2):220-31.
Applications: ELISA (Standard)

Novel antiviral activity of chemokines.
Authors: Nakayama T, Shirane J, Hieshima K, Shibano M, Watanabe M, Jin Z, Nagakubo D, Saito T, Shimomura Y, Yoshie O
Virology, 2006-04-17;350(2):484-92.
Species: Virus
Sample Types: Virus, Whole Cells
Applications: Binding Assay, Bioassay

Changes in soluble factor-mediated CD8+ cell-derived antiviral activity in cynomolgus macaques infected with simian immunodeficiency virus SIVmac251: relationship to biological markers of progression.
Authors: Dioszeghy V, Benlhassan-Chahour K, Delache B, Dereuddre-Bosquet N, Aubenque C, Gras G, Le Grand R, Vaslin B
J. Virol., 2006-01-01;80(1):236-45.
Applications: ELISA (Standard)

Inhibition of the development of collagen-induced arthritis in rhesus monkeys by a small molecular weight antagonist of CCR5.
Authors: Vierboom MP, Zavodny PJ, Chou CC, Tagat JR, Pugliese-Sivo C, Strizki J, Steensma RW, McCombie SW, Celebi-Paul L, Remarque E, Jonker M, Narula SK, Hart B
Arthritis Rheum., 2005-02-01;52(2):627-36.
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Whole Cells
Applications: Bioassay

CCR5-binding chemokines modulate CXCL12 (SDF-1)-induced responses of progenitor B cells in human bone marrow through heterologous desensitization of the CXCR4 chemokine receptor.
Authors: Honczarenko M, Le Y, Glodek AM, Silberstein LE
Blood, 2002-10-01;100(7):2321-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Induction of inhibitory antibodies to the CCR5 chemokine receptor and their complementary role in preventing SIV infection in macaques.
Authors: Lehner T, Wang Y, Doyle C, Tao L, Bergmeier LA, Mitchell E, Bogers WM, Heeney J, Kelly CG
Eur. J. Immunol., 1999-08-01;29(8):2427-35.
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Whole Cells
Applications: Bioassay