Recombinant Human CCL24/Eotaxin-2/MPIF-2 Protein
Recombinant Human CCL24/Eotaxin-2/MPIF-2 Protein Summary
Product Specifications
Val27-Cys119
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
343-E2
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 20 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
343-E2/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: CCL24/Eotaxin-2/MPIF-2
Eotaxin, also named MPIF-2 and Ck beta 6, is a novel CC chemokine identified in the Human Genome Sciences, Inc. database based on the presence of the CC motif and homology with other known CC chemokines. Eotaxin-2 cDNA encodes a 119 amino acid residue precursor protein with a 26 aa residue signal peptide that is cleaved to generate a mature protein predicted to contain 93 amino acid residues with an N-glycosylation site. Although one recombinant preparation of MPIF-2/Eotaxin-2 from insect cells was reported to be glycosylated and of the predicted size (10.5 kDa), a second preparation of recombinant Eotaxin-2 prepared by a different group, also from insect cells, was shown to contain a 78 amino acid residue carboxy-terminally truncated variant of Eotaxin-2. Additional minor carboxy-terminally truncated variants with 73, 75 and 76 residues were also isolated. Compared to other CC chemokines, Eotaxin-2 exhibits 40%, 42% and 39% amino acid identity to MCP-3, MIP-1 alpha, and Eotaxin, respectively. Eotaxin-2 mRNA is weakly expressed in activated monocytes and T lymphocytes.
Recombinant Eotaxin-2 has been shown to induce chemotaxis of eosinophils, basophils, and resting T lymphocytes but not monocytes and activated T lymphocytes. Eotaxin-2 has also been shown to suppress the colony formation by the high proliferative potential colony-forming cells which represent multipotential hematopoietic progenitors. On eosinophils, the effects of Eotaxin-2 was shown to be inhibited by an anti-CCR-3 antibody and to be cross-desensitized by Eotaxin or MCP-4, suggesting that all three CC chemokines act through CCR-3, at least on eosinophils.
- Forssmann, U. et al. (1997) J. Exp. Med. 185:2171.
- Patel, V.P. et al. (1997) J. Exp. Med. 185:1163.
Citations for Recombinant Human CCL24/Eotaxin-2/MPIF-2 Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Physiologic concentrations of HMGB1 have no impact on cytokine-mediated eosinophil survival or chemotaxis in response to Eotaxin-2 (CCL24).
Authors: Dyer, Kimberly, Rosenberg, Helene F
PLoS ONE, 2015-03-16;10(3):e0118887.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Chemotaxis of bone marrow derived eosinophils in vivo: a novel method to explore receptor-dependent trafficking in the mouse.
Authors: Sturm E, Dyer K, Percopo C, Heinemann A, Rosenberg H
Eur J Immunol, 2013-06-14;43(8):2217-28.
Species: Mouse
Sample Types: In Vivo
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Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
Nature, 2012-07-26;487(7408):505-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Coexpression of IL-5 and eotaxin-2 in mice creates an eosinophil-dependent model of respiratory inflammation with characteristics of severe asthma.
Authors: Ochkur SI, Jacobsen EA, Protheroe CA, Biechele TL, Pero RS, McGarry MP, Wang H, O'Neill KR, O'Neill KR, Colbert DC, Colby TV, Shen H, Blackburn MR, Irvin CC, Lee JJ, Lee NA
J. Immunol., 2007-06-15;178(12):7879-89.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Functional analysis of the chemokine receptor CCR3 on airway epithelial cells.
Authors: Beck LA, Tancowny B, Brummet ME, Asaki SY, Curry SL, Penno MB, Foster M, Bahl A, Stellato C
J. Immunol., 2006-09-01;177(5):3344-54.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
CD16+ monocyte-derived macrophages activate resting T cells for HIV infection by producing CCR3 and CCR4 ligands.
Authors: Ancuta P, Autissier P, Wurcel A, Zaman T, Stone D, Gabuzda D
J. Immunol., 2006-05-15;176(10):5760-71.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
CC chemokines and transmigration of eosinophils in the presence of vascular cell adhesion molecule 1.
Authors: Yamamoto H, Nagata M, Sakamoto Y
Ann. Allergy Asthma Immunol., 2005-02-01;94(2):292-300.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Bioassay -
Identification of full, partial and inverse CC chemokine receptor 3 agonists using [35S]GTPgammaS binding.
Authors: Wan Y, Jakway JP, Qiu H, Shah H, Garlisi CG, Tian F, Ting P, Hesk D, Egan RW, Billah MM, Umland SP
Eur. J. Pharmacol., 2002-12-05;456(1):1-10.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
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