Recombinant Human CCL16/HCC-4 Protein Summary
Product Specifications
Gln24-Gln120
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
802-HC
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
802-HC/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: CCL16/HCC-4
Human HCC-4, also named NCC-4, liver-expressed chemokine (LEC), and lymphocyte and monocyte chemoattractant (LMC), is a novel CC chemokine identified through bioinformatics. HCC-4 cDNA encodes a 120 amino acid (aa) residue precursor protein with a 23 aa residue predicted signal peptide that is cleaved to generate a 97 aa residue mature protein. HCC-4 is distantly related to other CC chemokines, exhibiting less than 30% aa sequence identity. Among these CC chemokines, HCC-4 has the most similarity to HCC-1. Two potential polyadenylation signals are present on the human HCC-4 gene, and as a result, two transcripts containing approximately 1,500 base pairs and 500 base pairs have been detected. HCC-4 is expressed weakly by some lymphocytes, including NK cells, gamma δ T cells, and some T cell clones. The expression of HCC-4 in monocytes is highly upregulated in the presence of IL-10. The HCC-4 gene has been mapped to chromosome 17q where multiple CC chemokines are clustered.
Recombinant HCC-4 has been shown to chemoattract human monocytes and THP-1 cells but not resting lymphocytes or neutrophils. HCC-4 has also been found to suppress proliferation of myeloid progenitor cells. The HCC-4 induced calcium flux in THP-1 cells can be desensitized by prior exposure to RANTES, suggesting that HCC-4 and RANTES share the same receptor in THP-1 cells.
- Shoudai, K. et al. (1998) Biochim. Biophys. Acta 1396:273.
- Hedrick, J. et al. (1998) Blood 91:4242.
- Youn, B-S. et al. (1998) BBRC 247:217.
Citations for Recombinant Human CCL16/HCC-4 Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Distinct Plasma Immune Profile in ALS Implicates sTNFR-II in pAMPK/Leptin Homeostasis
Authors: V Picher-Mar, H Boutej, A Vézina, P Cordeau, H Kaneb, JP Julien, A Genge, N Dupré, J Kriz
International Journal of Molecular Sciences, 2023-03-07;24(6):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
CCR8(+)FOXp3(+) Treg cells as master drivers of immune regulation
Authors: Y Barsheshet, G Wildbaum, E Levy, A Vitenshtei, C Akinseye, J Griggs, SA Lira, N Karin
Proc. Natl. Acad. Sci. U.S.A., 2017-05-22;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
Nature, 2012-07-26;487(7408):505-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Regulation of human LZIP expression by NF-kappaB and its involvement in monocyte cell migration induced by Lkn-1.
Authors: Jang SW, Kim YS, Kim YR, Sung HJ, Ko J
J. Biol. Chem., 2007-02-12;282(15):11092-100.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Novel antiviral activity of chemokines.
Authors: Nakayama T, Shirane J, Hieshima K, Shibano M, Watanabe M, Jin Z, Nagakubo D, Saito T, Shimomura Y, Yoshie O
Virology, 2006-04-17;350(2):484-92.
Species: Virus
Sample Types: Virus, Whole Cells
Applications: Binding Assay, Bioassay -
CCL16/LEC powerfully triggers effector and antigen-presenting functions of macrophages and enhances T cell cytotoxicity.
Authors: Cappello P, Caorsi C, Bosticardo M
J. Leukoc. Biol., 2003-10-02;75(1):135-42.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
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