Recombinant Human CCL11/Eotaxin Protein Summary
Product Specifications
Gly24-Pro97
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
320-EO
Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. |
Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
320-EO/CF
Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. |
Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: CCL11/Eotaxin
CCL11 is a potent eosinophil chemoattractant that was originally purified from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol challenge with ovalbumin. Microsequencing of the purified protein revealed the guinea pig CCL11 to be a member of the beta (CC) chemokine family of inflammatory and immunoregulatory cytokines. cDNA clones for guinea pig, mouse and human CCL11 have been isolated. Human CCL11 cDNA encodes a 97 amino acid residue precursor protein from which the amino-terminal 23 amino acid residues are cleaved to generate the 74 amino acid residue mature human CCL11. At the protein sequence level, mature human CCL11 is approximately 60% identical to mature mouse and guinea pig CCL11. In addition, human CCL11 also shows high amino acid sequence identity to human MCP-1, 2 and 3. Human CCL11 is chemotactic for eosinophils, but not mononuclear cells or neutrophils. The CC chemokine receptor 3 (CCR3) has now been identified to be a specific human CCL11 receptor (1 ‑ 3). CCR3 has also been shown to serve as a cofactor for a restricted subset of primary HIV viruses and binding of CCL11 to CCR3 inhibited infection by the HIV isolates (4).
- Kitamura, M. et al. (1996) J. Biol. Chem 271:7725.
- Garcia-Zepeda, E.A. et al. (1996) Nature Medicine 2:449.
- Ponath, P.D. et al. (1996) J. Clin. Invest. 97:604.
- Choe, H. et al. (1996) Cell 85:1135.
Citations for Recombinant Human CCL11/Eotaxin Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
31
Citations: Showing 1 - 10
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IL-5 antagonism reverses priming and activation of eosinophils in severe eosinophilic asthma
Authors: Luo, J;Chen, W;Liu, W;Jiang, S;Ye, Y;Shrimanker, R;Hynes, G;Klenerman, P;Pavord, ID;Xue, L;
Mucosal immunology
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Role of CCR3 in respiratory syncytial virus infection of airway epithelial cells.
Authors: Wellemans V, Benhassou H, Fuselier E, Bellesort F, Dury S, Lebargy F, Dormoy V, Fichel C, Naour R, Gounni A, Lamkhioued B
iScience, 2021-11-14;24(12):103433.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Chemoattraction of Neoplastic Glial Cells with CXCL10, CCL2 and CCL11 as a Paradigm for a Promising Therapeutic Approach for Primary Brain Tumors
Authors: L Déry, G Charest, B Guérin, M Akbari, D Fortin
International Journal of Molecular Sciences, 2021-11-10;22(22):.
Species: Human, Rat
Sample Types: In Vivo, Whole Cells
Applications: Bioassay, In Vivo -
The Release Kinetics of Eosinophil Peroxidase and Mitochondrial DNA Is Different in Association with Eosinophil Extracellular Trap Formation
Authors: N Germic, T Fettrelet, D Stojkov, A Hosseini, MP Horn, A Karaulov, D Simon, S Yousefi, HU Simon
Cells, 2021-02-03;10(2):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
A field-applicable method for flow cytometric analysis of granulocyte activation: Cryopreservation of fixed granulocytes
Authors: K de Ruiter, S van Staver, B Hilvering, E Knol, N Vrisekoop, L Koenderman, M Yazdanbakh
Cytometry A, 2018-03-13;0(0):.
Species: Human
Sample Types: Whole Blood
Applications: Bioassay -
Adsorption as a Contributor for Inflammatory Mediators Removal by Different Hemofiltration Membranes: A Pilot Study
Authors: Benjamin Malard
Artif Organs, 2016-12-02;0(0):.
Species: Human
Sample Types: Plasma
Applications: Hemofiltration Procedure -
The soluble guanylyl cyclase activator BAY 60-2770 inhibits murine allergic airways inflammation and human eosinophil chemotaxis
Authors: Edson Antunes
Pulm Pharmacol Ther, 2016-11-03;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Extracellular Microvesicle Production by Human Eosinophils Activated by Inflammatory Stimuli
Authors: Rossana C N Melo
Front Cell Dev Biol, 2016-10-27;4(0):117.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Anti-Inflammatory Effects of Metformin Irrespective of Diabetes Status
Circ Res, 2016-07-14;0(0):.
Species: Human
Sample Types: Plasma
Applications: Bioassay -
Functional analysis of free fatty acid receptor GPR120 in human eosinophils: implications in metabolic homeostasis.
Authors: Konno, Yasunori, Ueki, Shigehar, Takeda, Masahide, Kobayashi, Yoshiki, Tamaki, Mami, Moritoki, Yuki, Oyamada, Hajime, Itoga, Masamich, Kayaba, Hiroyuki, Omokawa, Ayumi, Hirokawa, Makoto
PLoS ONE, 2015-03-19;10(3):e0120386.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Helminth infections and type 2 diabetes: a cluster-randomized placebo controlled SUGARSPIN trial in Nangapanda, Flores, Indonesia.
Authors: Tahapary D, de Ruiter K, Martin I, van Lieshout L, Guigas B, Soewondo P, Djuardi Y, Wiria A, Mayboroda O, Houwing-Duistermaat J, Tasman H, Sartono E, Yazdanbakhsh M, Smit J, Supali T
BMC Infect Dis, 2015-03-18;15(0):133.
Species: Human
Sample Types: Whole Blood
Applications: Bioassay -
Leukotriene B4 amplifies eosinophil accumulation in response to nematodes.
Authors: Patnode M, Bando J, Krummel M, Locksley R, Rosen S
J Exp Med, 2014-06-02;211(7):1281-8.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
NADPH oxidase-independent formation of extracellular DNA traps by basophils.
Authors: Morshed M, Hlushchuk R, Simon D, Walls A, Obata-Ninomiya K, Karasuyama H, Djonov V, Eggel A, Kaufmann T, Simon H, Yousefi S
J Immunol, 2014-04-25;192(11):5314-23.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Eotaxin-1 promotes prostate cancer cell invasion via activation of the CCR3-ERK pathway and upregulation of MMP-3 expression.
Authors: Zhu F, Liu P, Li J, Zhang Y
Oncol Rep, 2014-03-05;31(5):2049-54.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
The internal architecture of leukocyte lipid body organelles captured by three-dimensional electron microscopy tomography.
Authors: Melo, Rossana, Paganoti, Guillher, Dvorak, Ann M, Weller, Peter F
PLoS ONE, 2013-03-26;8(3):e59578.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Novel association between vasoactive intestinal peptide and CRTH2 receptor in recruiting eosinophils: a possible biochemical mechanism for allergic eosinophilic inflammation of the airways.
Authors: El-Shazly A, Begon D, Kustermans G, Arafa M, Dortu E, Henket M, Lefebvre P, Louis R, Delvenne P
J Biol Chem, 2012-11-20;288(2):1374-84.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
Nature, 2012-07-26;487(7408):505-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
The ageing systemic milieu negatively regulates neurogenesis and cognitive function.
Authors: Villeda SA, Luo J, Mosher KI, Zou B, Britschgi M, Bieri G, Stan TM, Fainberg N, Ding Z, Eggel A, Lucin KM, Czirr E, Park JS, Couillard-Despres S, Aigner L, Li G, Peskind ER, Kaye JA, Quinn JF, Galasko DR, Xie XS, Rando TA, Wyss-Coray T
Nature, 2011-08-31;477(7362):90-4.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Leptin enhances survival and induces migration, degranulation, and cytokine synthesis of human basophils.
Authors: Suzukawa M, Nagase H, Ogahara I, Han K, Tashimo H, Shibui A, Koketsu R, Nakae S, Yamaguchi M, Ohta K
J. Immunol., 2011-03-18;186(9):5254-60.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Osteopontin is expressed and functional in human eosinophils.
Authors: Puxeddu I, Berkman N, Ribatti D
Allergy, 2009-10-05;65(2):168-74.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases.
Authors: Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D
J. Immunol., 2008-12-01;181(11):8053-67.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Maternal and cord plasma cytokine and chemokine profile in pregnancies complicated by asthma.
Authors: Osei-Kumah A, Smith R, Clifton VL
Cytokine, 2008-07-17;43(2):187-93.
Applications: ELISA (Standard) -
Regulation of D6 chemokine scavenging activity by ligand- and Rab11-dependent surface up-regulation.
Authors: Bonecchi R, Borroni EM, Anselmo A, Doni A, Savino B, Mirolo M, Fabbri M, Jala VR, Haribabu B, Mantovani A, Locati M
Blood, 2008-05-14;112(3):493-503.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Functional analysis of the chemokine receptor CCR3 on airway epithelial cells.
Authors: Beck LA, Tancowny B, Brummet ME, Asaki SY, Curry SL, Penno MB, Foster M, Bahl A, Stellato C
J. Immunol., 2006-09-01;177(5):3344-54.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
Authors: Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B
Clin. Immunol., 2006-06-16;120(2):220-31.
Applications: ELISA (Standard) -
Novel antiviral activity of chemokines.
Authors: Nakayama T, Shirane J, Hieshima K, Shibano M, Watanabe M, Jin Z, Nagakubo D, Saito T, Shimomura Y, Yoshie O
Virology, 2006-04-17;350(2):484-92.
Species: Virus
Sample Types: Virus, Whole Cells
Applications: Binding Assay, Bioassay -
The CC chemokine eotaxin/CCL11 has a selective profibrogenic effect on human lung fibroblasts.
Authors: Puxeddu I, Bader R, Piliponsky AM, Reich R, Levi-Schaffer F, Berkman N
J. Allergy Clin. Immunol., 2005-12-02;117(1):103-10.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Manipulation of host cytokine network by ticks: a potential gateway for pathogen transmission.
Authors: Hajnicka V, Vancova I, Kocakova P, Slovak M, Gasperik J, Slavikova M, Hails RS, Labuda M, Nuttall PA
Parasitology, 2005-03-01;130(0):333-42.
Applications: ELISA (Standard) -
CC chemokines and transmigration of eosinophils in the presence of vascular cell adhesion molecule 1.
Authors: Yamamoto H, Nagata M, Sakamoto Y
Ann. Allergy Asthma Immunol., 2005-02-01;94(2):292-300.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Bioassay -
Identification of full, partial and inverse CC chemokine receptor 3 agonists using [35S]GTPgammaS binding.
Authors: Wan Y, Jakway JP, Qiu H, Shah H, Garlisi CG, Tian F, Ting P, Hesk D, Egan RW, Billah MM, Umland SP
Eur. J. Pharmacol., 2002-12-05;456(1):1-10.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Chemoattractant factors in breast milk from allergic and nonallergic mothers.
Authors: Bottcher MF, Jenmalm MC, Bjorksten B, Garofalo RP
Pediatr. Res., 2000-05-01;47(5):592-7.
Applications: ELISA (Standard)
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