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Recombinant Human Cardiotrophin-1/CT-1 Protein

Catalog #: 612-CD
9 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
612-CD-010/CF
612-CD-050/CF

With Carrier

Catalog # Availability Size / Price Qty
612-CD-010
612-CD-050
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (9)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Cardiotrophin-1/CT-1 Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.25‑1.25 ng/mL.
Source
E. coli-derived human Cardiotrophin-1/CT-1 protein
Ser2-Ala201
Accession #
Q16619
N-terminal Sequence
Analysis
Ser2
Predicted Molecular Mass
21 kDa

Product Datasheets

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612-CD (with carrier)

Product Datasheet
COA
SDS

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612-CD/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

612-CD

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCL containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

612-CD/CF

Formulation Supplied as a 0.2 μm filtered solution in Acetonitrile and TFA.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cardiotrophin-1/CT-1

Cardiotrophin-1 (CT-1) is a member of the cytokine family which also includes IL-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), and ciliary neurotrophic factor (CNTF). It was originally isolated based on its ability to induce cardiac myocyte hypertrophy in vitro. CT-1 has since been shown to be a pleiotrophic cytokine with overlapping actions with other IL-6 family members on a variety of cell types. Human CT-1 encodes a 201 amino acid (aa) residue protein that lacks a hydrophobic signal peptide. The mechanism of CT-1 release from cells is currently not understood. Human and mouse CT-1 share 80% aa sequence identity and exhibit cross-species activity. CT-1 is highly expressed in heart, skeletal muscle, liver, lung and kidney. Lower levels of CT-1 expression is also seen in testis and brain. CT-1 initiates downstream signaling pathways through the heterodimerization of gp130 and the LIF receptor beta subunit. A third  alpha receptor subunit has been implicated in the receptor complex.

References
  1. Pennica, D. et al. (1996) Cytokine and Growth Factor Reviews 7:81.
  2. Robledo, O. et al. (1997) J. Biol. Chem. 272:4855.
Entrez Gene IDs
1489 (Human); 13019 (Mouse)
Alternate Names
cardiophin 1; cardiotrophin 1; Cardiotrophin1; Cardiotrophin-1; CT-1CT1; CTF1

Citations for Recombinant Human Cardiotrophin-1/CT-1 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Monitoring phosphorylation and acetylation of CRISPR-mediated HiBiT-tagged endogenous proteins
    Authors: Alves, J;Schwinn, M;Machleidt, T;Goueli, SA;Cali, JJ;Zegzouti, H;
    Scientific reports
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Pleiotropic, Unique and Shared Responses Elicited by IL-6 Family Cytokines in Human Vascular Endothelial Cells
    Authors: M Lindkvist, MM Zegeye, M Grenegård, LU Ljungberg
    International Journal of Molecular Sciences, 2022-01-27;23(3):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Differential Proteomics Identifies Reticulocalbin-3 as a Novel Negative Mediator of Collagen Production in Human Cardiac Fibroblasts
    Authors: E Martínez-M, J Ibarrola, A Fernández-, E Santamaria, J Fernández-, P Rossignol, F Jaisser, N López-Andr
    Sci Rep, 2017-09-22;7(1):12192.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Efficient differentiation of human embryonic and induced pluripotent stem cells into functional astrocytes.
    Authors: Emdad L, D'Souza SL, Kothari HP, Qadeer ZA, Germano IM
    Stem Cells Dev., 2011-07-26;21(3):404-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Sortilin facilitates signaling of ciliary neurotrophic factor and related helical type 1 cytokines targeting the gp130/leukemia inhibitory factor receptor beta heterodimer.
    Authors: Larsen JV, Hansen M, Moller B, Madsen P, Scheller J, Nielsen M, Petersen CM
    Mol. Cell. Biol., 2010-06-28;17(0):4175.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Interferon-gamma reverses the immunosuppressive and protumoral properties and prevents the generation of human tumor-associated macrophages.
    Authors: Duluc D, Corvaisier M, Blanchard S, Catala L, Descamps P, Gamelin E, Ponsoda S, Delneste Y, Hebbar M, Jeannin P
    Int. J. Cancer, 2009-07-15;125(2):367-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Cardiotrophin-1 induces interleukin-6 synthesis in human monocytes.
    Authors: Fritzenwanger M, Meusel K, Foerster M, Kuethe F, Krack A, Figulla HR
    Cytokine, 2007-07-16;38(3):137-44.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Cardiotrophin-1 in choroid plexus and the cerebrospinal fluid circulatory system.
    Authors: Gard AL, Gavin E, Solodushko V, Pennica D
    Neuroscience, 2004-01-01;127(1):43-52.
    Applications: Western Blot
  9. Induction of protein synthesis in cardiac fibroblasts by cardiotrophin-1: integration of multiple signaling pathways.
    Authors: Freed DH, Borowiec AM, Angelovska T
    Cardiovasc. Res., 2003-11-01;60(2):365-75.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay

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