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Recombinant Human C1qTNF14/C1qL1 Protein, CF

Catalog #: 9134-TN Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
9134-TN-050
Recombinant Human C1qTNF14/C1qL1 Protein Bioactivity
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Recombinant Human C1qTNF14/C1qL1 Protein, CF Summary

Product Specifications

Purity
>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human C1qTNF14/C1qL1 is immobilized at 1 μg/mL, 100 μL/well, the concentration of Recombinant Human BAI3 (Catalog # 9106-BA) that produces 50% of the optimal binding response is approximately 0.4-2.4 μg/mL
Source
E. coli-derived human C1qL1 protein
Thr125-Asp258, with an N-terminal Met and 6-His tag
Accession #
O75973
N-terminal Sequence
Analysis
Met
Structure / Form
Noncovalently-linked homotrimer
Predicted Molecular Mass
16 kDa
SDS-PAGE
13 kDa, reducing conditions

Product Datasheets

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9134-TN

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9134-TN

Formulation Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and TCEP.
Reconstitution Reconstitute at 1 mg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Human C1qTNF14/C1qL1 Protein Bioactivity View Larger

When Recombinant Human C1qTNF14/C1qL1 (Catalog # 9134-TN) is coated at 1 µg/mL (100 µL/well), Recombinant Human BAI3 (Catalog # (Catalog # 9106-BA) binds with a typical ED50 of 0.4-2.4 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: C1qL1

C1qTNF14 (CTRP14), also known as C1qL1, is an approximately 30 kDa member of the C1q family of secreted proteins (1, 2). Mature human C1qTNF14 contains a collagen-like region and one C1q-like domain and can form disulfide-linked heteromers with C1qTNF11/C1qL4 (3, 4). Within the C1q-like domain, human C1qTNF14 shares 100% aa sequence identity with mouse and rat C1qTNF14. C1qTNF14 is expressed in the inferior olive, hippocampus, and cerebral cortex (5). Similarly to C1qTNF13/C1qL3, C1qTNF10/C1qL2, and C1qTNF11/C1qL4, C1qTNF14 binds to BAI3 in the cerebral cortex and on cerebellar Purkinje cells (5-8). C1qTNF14/C1qL1 binding to BAI3 induces the formation and maintenance of excitatory synapses between climbing fibers and parallel fibers with Purkinje cells (5, 7).

References
  1. Grebrehiwet, B. et al. (2012) Front. Immunol. 5:3.
  2. Yuzaki, M. (2010) Eur. J. Neurosci. 32:191.
  3. Ressl, S. et al. (2015) Structure 23:688.
  4. Wei, Z. et al. (2013) J. Biol. Chem. 288:10214.
  5. Sigoillot, S.M. et al. (2015) Cell Rep. 10:820.
  6. Lanoue, V. et al. (2013) Mol. Psychiatry 18:943.
  7. Kakegawa, W. et al. (2015) Neuron 85:316.
  8. Bolliger, M.F. et al. (2011) Proc. Natl. Acad. Sci. USA 108:2534.
Long Name
Complement Component 1, Q Subcomponent-Like 1
Entrez Gene IDs
10882 (Human); 23829 (Mouse); 363686 (Rat)
Alternate Names
C1qL1; C1q-related factor; C1QRF; C1QRFCRFC1QTNF14; C1qTNF14; Complement component 1 Q subcomponent-like 1; complement component 1, q subcomponent-like 1; CTRP14

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