Home / Butyrylcholinesterase/BCHE / Recombinant Human Butyrylcholinesterase/BCHE Protein, CF

Recombinant Human Butyrylcholinesterase/BCHE Protein, CF

Catalog #: 6137-CE Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
6137-CE-010
R&D Systems Recombinant Proteins and Enzymes
1 Image
Product Details
FAQs
Reviews
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Butyrylcholinesterase/BCHE Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to cleave Butyrylthiocholine. The specific activity is >50,000 pmol/min/μg, as measured under the described conditions.
Source
Chinese Hamster Ovary cell line, CHO-derived human Butyrylcholinesterase/BCHE protein
Met1-Leu602, with a C-terminal 6-His tag
Accession #
P06276
N-terminal Sequence
Analysis
Glu29
Structure / Form
Monomer and disulfide-linked homodimers
Predicted Molecular Mass
66 kDa
SDS-PAGE
90-100 kDa, reducing conditions

Product Datasheets

You must select a language.

x

6137-CE

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6137-CE

Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Assay Procedure

Materials
  • Assay Buffer: 100 mM Sodium Phosphate, pH 7.5
  • Recombinant Human Butyrylcholinesterase/BCHE (rhBCHE) (Catalog # 6137-CE)
  • Substrate: Butyrylthiocholine chloride (BTC) (Sigma Catalog # B3128), 20 mM stock in DMSO
  • 5,5’-dithio-bis (2-nitrobenzoic acid) (DTNB) (Sigma Catalog # D8130), 10 mM stock in DMSO
  • 96 well Clear Plate (Costar, Catalog # 92592)
  • Plate Reader (Model: SpectraMax Plus by Molecular Devices) or equivalent
  1. Dilute rhBCHE to 0.02 µg/mL in Assay Buffer.
  2. Dilute BTC and DTNB to 200 µM final concentrations in deionized water.
  3. Load into plate 50 µL of 0.02 µg/mL rhBCHE and start the reaction by adding 50 µL of the BTC/DTNB mixture to the wells. As a Substrate Blank, load 50 µL of Assay Buffer and 50 µL of the BTC/DTNB mixture.
  4. Read in kinetic mode for 5 minutes at an absorbance of 405 nm.
  5. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

 Adjusted Vmax* (OD/min) x well volume (L) x 1012 pmol/mol
ext. coeff** (M-1cm-1) x path corr.*** (cm) x amount of enzyme (µg)

     *Adjusted for Substrate Blank 
     **Using the extinction coefficient 13260 M-1cm-1 
     ***Using the path correction 0.32 cm
     Note: the output of many spectrophotometers is in mOD Per Well:
  • rhBCHE: 0.001 µg
  • DTNB and BTC: 100 µM
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Butyrylcholinesterase/BCHE

Butyrylcholinesterase (BCHE) is a major acetylcholine hydrolyzing enzyme in the circulation (1). Although it is present in significant amounts in human plasma, no endogenous physiological substrate has been described for this enzyme. It can degrade a large number of ester-containing compounds besides acylcholines, including neurotoxic organophosphate esters. Thus, it plays significant pharmacological and toxicological roles. It is thought to be involved in the pathological progression of Alzheimer’s disease (AD) by depleting acetylcholine. In contrast to ACHE, it attenuates amyloid fibril formation in vitro (2). BCHE inhibitors have been used to delay symptoms of AD patients by virtue of their ability to enhance ACH availability (3). Its involvement in the cholinergic anti-inflammatory pathway connects BCHE and ACHE as possible markers of low-grade systemic inflammation observed in Type-2 diabetes, obesity, hypertension, coronary heart disease, and AD (4). BCHE can exist as monomers, dimers, or tetramers (1).

References
  1. Darvesh, S. et al. (2003) Nat. Rev. Neurosci. 4:131.
  2. Diamant, S. et al. (2006) Proc. Nat. Acad. Sci. 103:8628.
  3. Campbell, V. A. and  Gowran, A. (2007) Br. J. Pharm. 152:655.
  4. Das, U. N. (2007) Med Sci Monit. 13:RA214.
Entrez Gene IDs
590 (Human); 12038 (Mouse)
Alternate Names
Acylcholine acylhydrolase; BCHE; Butyrylcholine esterase; Butyrylcholinesterase; CHE1; CHE1cholinesterase; Choline esterase II; cholinesterase 1; E1; EC 3.1.1.8; Pseudocholinesterase

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Human Butyrylcholinesterase/BCHE Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human Butyrylcholinesterase/BCHE Protein, CF and earn rewards!

Have you used Recombinant Human Butyrylcholinesterase/BCHE Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review