Recombinant Human BTN2A1/Butyrophilin 2A1 Protein, CF Summary
Product Specifications
0.1-0.5 μg/mL.
Gln29-Ala248, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9058-BT
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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When Recombinant Human BTN2A1/Butyrophilin 2A1 (Catalog # 9058-BT) is coated at 1 µg/mL, Recombinant Human DC-SIGN/CD209 Fc Chimera (Catalog # 161-DC) binds with a ED50 of 0.1-0.5 μg/mL.
Reconstitution Calculator
Background: BTN2A1
Butyrophilin 2A1 (BTN2A1) is an approximately 70 kDa widely expressed and variably glycosylated type I transmembrane glycoprotein (1). Mature human Butyrophilin 2A1 consisits of a 220 amino acid (aa) extracellular domain with two immunoglobulin-like domains, a 21 aa transmembrane segment, and a 258 aa cytoplasmic domain (2). Alternative splicing generates additional isoforms of human Butyrophilin 2A1 that lack the first Ig-like domain or transmembrane segment as well as isoforms with substitutions and deletions in the cytoplasmic region. BTN2A1 is widely expressed including on colonic epithelial cells, on immune cells, and in milk fat globules (3, 4). It binds to the C-type lectin DC-SIGN on monocyte-derived dendritic cells, and this interaction can be blocked by soluble gp130 from HIV (3). The polymorphism of BTN2A1 has been associated with metabolic syndrome, type II diabetes mellitus, chronic kidney disease, and hypertension (5, 6).
- Arnett, H.A. and J.L. Viney (2014) Nat. Rev. Immunol. 14:559.
- Tazi-Ahnini, R. et al. (1997) Immunogenetics 47:55.
- Malcherek, G. et al. (2007) J. Immunol. 179:3804.
- Cavaletto, M. et al. (2002) Proteomics 2:850.
- Oguri, M. et al.(2011) J. Med. Genet. 48:787.
- Horibe, H. et al. (2014) Mol. Med. Rep. 9:808.
Citation for Recombinant Human BTN2A1/Butyrophilin 2A1 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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BTN2A1, an immune checkpoint targeting Vgamma9Vdelta2 T cell cytotoxicity against malignant cells
Authors: CE Cano, C Pasero, A De Gassart, C Kerneur, M Gabriac, M Fullana, E Granarolo, R Hoet, E Scotet, C Rafia, T Hermman, C Imbert, L Gorvel, N Vey, A Briantais, AC le Floch, D Olive
Cell Reports, 2021-07-13;36(2):109359.
Species: Human, Mouse
Sample Types: Cell Lysates, In Vivo
Applications: ELISA Capture, Immunization
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