Recombinant Human beta-Glucuronidase/GUSB Protein, CF
Recombinant Human beta-Glucuronidase/GUSB Protein, CF Summary
Product Specifications
The specific activity is >4,000 pmol/min/μg, as measured under the described conditions.
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
6144-GH
| Formulation | Supplied as a 0.2 μm filtered solution in Tris and NaCl. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date. |
Reconstitution Calculator
Background: beta-Glucuronidase/GUSB
Human beta -Glucuronidase (EC 3.2.1.31) encoded by the GUSB gene is a lysosomal hydrolase involved in the stepwise degradation of glucuronic acid-containing glycosaminoglycans that include heparan sulfate, chondroitin sulfate and hyaluronan (1). The enzyme is only active on the glucuronic acid of the non-reducing end. The native protein has been reported as a tetrameric glycoprotein composed of identical subunits (1, 2). Mutations in the GUSB gene are linked to mucopolysaccharidosis type VII (3). Accumulation of partially degraded glycosaminoglycans, with glucuronic acid residues at the non-reducing termini, are usually found in the lysosomes of patients with the disease (3). It has also been reported that this enzyme may contribute to the depletion of chondroitin from cartilage and thereby facilitate the damage of joints in rheumatoid arthritis (4).
- Shipley, J.M. et al. (1993) Am. J. Hum. Genet. 52:517.
- Oshima, A, et al. (1987) Proc. Natl. Acad. Sci. USA 84:685.
- Bell, C.E. Jr. et al. (1977) J. Clin. Invest. 59:97.
- Ortutay, Z. et al. (2003) Arthritis Rheum. 48:2163.
Citation for Recombinant Human beta-Glucuronidase/GUSB Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Microbial ?-glucuronidases drive human periodontal disease etiology
Authors: Lietzan, AD;Simpson, JB;Walton, WG;Jariwala, PB;Xu, Y;Boynton, MH;Liu, J;Redinbo, MR;
Science advances
Species: Human
Sample Types: Gingival Crevicular Fluid (GCF)
Applications: Bioassay
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