Recombinant Human beta-Defensin 3 Protein, CF

Catalog #: 4435-BD
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
4435-BD-050

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human beta-Defensin 3 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its anti-microbial activity against E. coli. Ganz, T. et al. (2003) Nat. Rev. Immunol. 3:710. The ED₅₀ for this effect is 7.5-30 μg/mL.

Source

E. coli-derived human beta-Defensin 3 protein
Gly23-Lys67

Accession #

P81534

N-terminal Sequence
Analysis

Gly23

Predicted Molecular Mass

5.2 kDa

Product Datasheets

4435-BD

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4435-BD

Formulation	Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution	Reconstitute at 500 μg/mL in sterile 4 mM HCl.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: beta-Defensin 3

beta -Defensin 3, also known as BD3 and DEFB-3, is a membrane-active cationic peptide that functions in inflammation and innate immune responses. There are at least 30 beta-Defensins which are distinguished from alpha-Defensins by the connectivity pattern of their three intramolecular disulfide bonds (1). The 45 amino acid (aa) mature human BD3 shares 38% and 33% aa sequence identity with mouse and rat BD3, respectively (2, 3). It shares 18%-36% aa sequence identity with other human beta-Defensins. BD3 is widely expressed among epithelial tissues, notably by keratinocytes and airway epithelial cells. It is upregulated in response to proinflammatory cytokines, microbial and viral infections, and at the edges of skin wounds (2, 4-6). BD3 induction in osteoarthritis chondrocytes promotes MMP1 and 13 production and inhibits TIMP1 and 2 expression (7). In vivo control of BD3 activity is accomplished in part through cleavage by cathepsins B, L, and S (8). BD3 displays strain specific microbicidal activity toward a broad spectrum of bacteria and yeast (2, 9). BD3 also induces monocyte migration, mast cell activation, and a mast cell-dependent increase in vascular permeability (4, 10). Disruption of the intramolecular disulfide bond pattern in BD3 abrogates its monocyte chemoattractant properties but not its antimicrobial properties (11, 12). BD3 inhibits viral infectivity by interacting directly with HIV-1 plus its coreceptor CXCR4 (5, 13), and with HSV glycoprotein B plus its receptor heparan sulfate (14), and by forming a protective coating on the surface of influenza virus target cells (15).

References

Dhople, V. et al. (2006) Biochim. Biophys. Acta 1758:1499.
Harder, J. et al. (2001) J. Biol. Chem. 276:5707.
Schibli, D.J. et al. (2002) J. Biol. Chem. 277:8279.
Garcia, J.-R.C. et al. (2001) Cell Tissue Res. 306:257.
Quinones-Mateu, M.E. et al. (2003) AIDS 17:F39.
Sorensen, O.E. et al. (2006) J. Clin. Invest. 116:1878.
Varoga, D. et al. (2005) Arthritis Rheum. 52:1736.
Taggart, C.C. et al. (2003) J. Immunol. 171:931.
Joly, S. et al. (2004) J. Clin. Microbiol. 42:1024.
Chen, X. et al. (2007) Eur. J. Immunol. 37:434.
Kluver, E. et al. (2005) Biochemistry 44:9804.
Wu, Z. et al. (2003) Proc. Natl. Acad. Sci. 100:8880.
Feng, Z. et al. (2006) J. Immunol. 177:782.
Hazrati, E. et al. (2006) J. Immunol. 177:8658.
Leikina, E. et al. (2005) Nat. Immunol. 6:995.

Entrez Gene IDs

55894 (Human)

Alternate Names

BD14; BD3; BD-3; beta-defensin 103; betaDefensin 3; beta-Defensin 3; DEFB103; DEFB103A; DEFB103B; DEFB3; DEFB-3; DEFB3DEFB103A; Defensin, beta 103; defensin, beta 103B; Defensin-like protein; HBD-3; HBD3HBP3

Related Research Areas

Citations for Recombinant Human beta-Defensin 3 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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