Recombinant Human AS3MT Protein, CF Summary
Product Specifications
Ala2-Cys375, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9827-MT
| Formulation | Lyophilized from a 0.2 μm filtered solution in Tris and NaCl with Trehalose. |
| Reconstitution | Reconstitute at 500 μg/mL in water. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: AS3MT
Arsenite methyltransferase (AS3MT) is a cytosolic, 42 kDa enzyme that belongs to the methyltransferase superfamily and catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to arsenite. Arsenic exposure in humans results in a broad range of acute and chronic life-long health risks including cancer, diabetes, cardiovascular and neurological diseases (1-4). Specifically, in all regions of the brain, arsenic exposure can cause a dose-dependent accumulation of arsenic compounds that interfere with glucose transporter function and cause a neurotoxic effect (5). In contrast, some arsenic forms and metabolites of arsenic are effective as therapeutic treatments for cancer (4, 6-8). In humans, AS3MT biomethylation paradoxically detoxifies arsenic and produces carcinogenic metabolites (4, 9-10). Several polymorphisms in the AS3MT gene have been shown to affect the protein's catalytic activity and result in potentially increased risk of arsenic-related disease (11). AS3MT is expressed primarily in liver although it is present in lower abundance in other tissues (12). Expression is also observed in plaque-resident cells where it contributes to atherosclerosis (13) and a truncated isoform of AS3MT is found to have increased expression in the brain where it is associated with schizophrenia (14, 15).
- Abernathy, C.O. et al. (2003) J. Nutr. 133:1536S.
- Tchounwou, P.B. et al. (2003) Toxicol. Pathol. 31:575.
- States, J.C. et al. (2009) Toxicol. Sci. 107:312.
- Khairul, I. et al. (2017) Oncotarget 8:23905.
- Rodriguez, V.M. et al. (2005) Toxicol. Sci. 84:157.
- Dilda, P.J. and P.J Hogg (2007) Cancer Treat. Rev. 33:542.
- Lalleman-Breitenback, V. et al. (2012) Trends. Mol. Med. 18:36.
- Lengfedler, E. et al. (2012) Leukemia. 26:433.
- Ajees, A.A. et al. (2012) Biochemistry. 51:5476.
- Dheeman, D.S. et al. (2014) Chem. Res. Toxicol. 27:1979.
- Li, J. et al. (2017) Chem. Res. Toxicol. 30:1481.
- Kobayashi, Y. et al. (2007) Environ. Toxicol. Pharmacol. 23:115.
- Negro Silva, L.F. et al. (2017) Environ Health Perspect. 125:077001.
- Li, M. et al. (2016) Nat. Med. 22:649.
- Li, L. et al. (2016) Mol. Neuropsychiatry 2:213.
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