Recombinant Human Angiopoietin-like Protein 2/ANGPTL2, CF

Name: Recombinant Human Angiopoietin-like Protein 2/ANGPTL2, CF
Brand: R&D Systems
SKU: 9795-AN
Rating: 5 (1 reviews)

Catalog #: 9795-AN
1 Citation 1 Review Datasheet / COA / SDS

C-Terminal Fragment

Catalog #	Availability	Size / Price	Qty
9795-AN-050

Recombinant Human Angiopoietin-like Protein 2/ANGPTL2 Binding Activity

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All Angiopoietin-like Protein 2/ANGPTL2 Proteins and Enzymes

Product Details

Citations (1)

FAQs

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Reviews (1)

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Angiopoietin-like Protein 2/ANGPTL2, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human Angiopoietin‑like Protein 2/ANGPTL2 is immobilized at 1 μg/mL, 100 μL/well, the concentration of Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera Recombinant Human ILT4/CD85d Fc Chimera (Catalog # 2078-T4) that produces 50% of the optimal binding response is 6-48 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human Angiopoietin-like Protein 2/ANGPTL2 protein
Thr260-His493, with a C-terminal 6-His tag

Accession #

Q9UKU9.1

N-terminal Sequence
Analysis

Thr260

Predicted Molecular Mass

28 kDa

SDS-PAGE

28-36 kDa, under reducing conditions.

Product Datasheets

9795-AN

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9795-AN

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human Angiopoietin-like Protein 2/ANGPTL2 (Catalog # 9795-AN) is immobilized at 1 µg/mL, 100 µL/well, Recombinant Human ILT4/CD85d Fc Chimera (Catalog # 2078-T4) binds with an ED₅₀ of 6-48 ng/mL.

Reconstitution Calculator

Background: Angiopoietin-like Protein 2/ANGPTL2

Angiopoietin-like Protein 2 (ANGPTL2), also known as ARP2 is a secreted 56 kDa glycoprotein that contains a N-terminal coiled coil domain and a C-terminal fibrinogen like domain (1). ANGPTL2 is one of the seven members of the Angiopoietin Like family proteins, which are structurally similar to angiopoietins (2). Within mature human ANGPTL2, amino acid 260-493 contains the C-terminal fibrinogen like domain, and this domain shares 99% sequence identity with both mouse and rat homologs. ANGPTL2 is widely expressed in many tissues, and its expression is induced by chronic, but not acute hypoxia (3). Elevated level of ANGPTL2 in the serum is also correlated with inflammation and obesity (3). ANGPTL2 has both pro and anti-angiogenic functions (1, 4). It serves as tumor suppressor in ovarian cancer (5), and also promote metastasis in various cancer types, making it potential biomarker for tumor progression (6-7). ANGPTL2 also function as growth factor to enhance the survival of hematopoietic progenitors, mediated by its coiled coil domain (8). Receptor of ANGPTL2 is recently identified as LILRB2, and both the coiled coil domain and fibrinogen like domain are required for optimal binding (9).

References

Kim, I. et al. (1999) J Biol Chem 274:26523.
Santulli, G. et al. (2014) Front Endocrinol 5:4.
Tabata, M. et al. (2009) Cell Metab 10:178.
Kubota, Y. et al. (2005) Proc. Natl. Acad. Sci. U.S.A. 102:13502.
Kikuchi, R. et al. (2008) Cancer Res 68:5067.
Odagiri, H. et al. (2014) Sci Signal 7:ra7.
Endo, M. et al. (2012) Cancer Res 72:1784.
Broxmeyer, H.E. et al. (2012) Blood Cells Mol Dis 48:25.
Deng, M. et al. (2014) blood 124:924.

Entrez Gene IDs

23452 (Human); 26360 (Mouse)

Alternate Names

angiopoietin-like 2; Angiopoietin-like Protein 2; angiopoietin-related protein 2; ANGPTL2; ANGRP2; ARP2HARP; MGC8889

Related Research Areas

Citation for Recombinant Human Angiopoietin-like Protein 2/ANGPTL2, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1