Recombinant Human Angiopoietin-like 2 Protein, CF

Catalog #: 9746-AN/CF Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9746-AN-025/CF

Recombinant Human Angiopoietin-like 2 Protein Binding Activity

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All Angiopoietin-like Protein 2/ANGPTL2 Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Angiopoietin-like 2 Protein, CF Summary

Product Specifications

Purity

>75%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Angiopoietin‑like Protein 2/ANGPTL2 is coated at 2 μg/mL, Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera (Catalog # 2078‑T4) binds with an ED₅₀ of 0.02‑0.12 μg/mL Measured by its ability to promote the expansion of E16 rat liver mononuclear cells in vitro, in the presence of Recombinant Mouse SCF/c‑kit Ligand (Catalog # 455-MC), Recombinant Mouse Thrombopoietin/Tpo (Catalog # 488-TO), and Recombinant Mouse Flt‑3 Ligand (Catalog # 427-FL). The ED₅₀ for this effect is 0.3-1.8 μg/mL.

Source

Mouse myeloma cell line, NS0-derived human Angiopoietin-like Protein 2/ANGPTL2 protein
Gln23-His493, with a C-terminal 9-His tag

Accession #

Q9UKU9.1

N-terminal Sequence
Analysis

No results obtained. Gln23 inferred from enzymatic pyroglutamate treatment revealing Glu24

Structure / Form

Disulfide-linked homooligomer

Predicted Molecular Mass

56 kDa

SDS-PAGE

60-69 kDa, reducing conditions

Product Datasheets

9746-AN/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9746-AN/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and CHAPS with Trehalose.
Reconstitution	Reconstitute at 200 μg/mL in water.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Angiopoietin-like Protein 2/ANGPTL2 (Catalog # 9746-AN) is coated at 2 µg/mL, Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera (Catalog # 2078-T4) binds with an ED₅₀ of 0.02-0.12 µg/mL.

Reconstitution Calculator

Background: Angiopoietin-like Protein 2/ANGPTL2

Angiopoietin-like Protein 2 (ANGPTL2), also known as ARP2, is a secreted 56 kDa glycoprotein that contains a coiled coil domain and a fibrinogen like domain (1). ANGPTL2 is one of the eight members of the Angiopoietin like family proteins, which are structurally similar to angiopoietins (2). Mature human ANGPTL2 shares 89% sequence identity with both mouse and rat homologs. ANGPTL2 is widely expressed in many tissues, and its expression is induced by chronic, but not acute hypoxia (3). Elevated level of ANGPTL2 in the serum is also correlated with inflammation and obesity (3). ANGPTL2 has both pro and anti-angiogenic functions (1, 4). It serves as tumor suppressor in ovarian cancer (5), and also promote metastasis in various cancer types, making it potential biomarker for tumor progression (6, 7). ANGPTL2 functions as growth factor to enhance the survival of hematopoietic progenitors, mediated by its coiled-coil domain (8). Receptor of ANGPTL2 is recently identified to be LILRB2, and both the coiled-coil domain and fibrinogen like domain are required for optimal binding (9).

References