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Recombinant Human Activin RIIB Fc Chimera (NS0-expressed)

Catalog #: 339-RB
5 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
339-RB-100/CF

With Carrier

Catalog # Availability Size / Price Qty
339-RB-100
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Product Details
Citations (5)
FAQs
Reviews
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Activin RIIB Fc Chimera (NS0-expressed) Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit Activin A-induced hemoglobin expression in K562 human chronic myelogenous leukemia cells. Schwall, R.H. et al. (1991) Method Enzymol. 198:340. Approximately 0.3-1 µg/mL of rhActivin RIIB/Fc Chimera will inhibit 50% of the biological response due to 3 ng/mL of rhActivin A.
Source
Mouse myeloma cell line, NS0-derived human Activin RIIB protein
Met Human Activin RIIB
(Ser19 - Thr 134)
Accession # CAA54671		 IEGRMD Human IgG1
(Pro100 - Lys330)		 6-His tag
N-terminus C-terminus
Accession #
CAA54671
N-terminal Sequence
Analysis
Met
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
41 kDa (monomer)
SDS-PAGE
60 kDa, reducing conditions

Product Datasheets

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339-RB (with carrier)

Product Datasheet
COA
SDS

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339-RB/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

339-RB

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

339-RB/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Activin RIIB

Activin isoforms and other members of the TGF-beta  superfamily exert their biological effects by binding to heteromeric complexes of a type I and a type II serine-threonine kinase receptor, both of which are essential for signal transduction. Seven type I and five type II receptors, including the two type I and the two type II activin receptors, designated ActR-I(A), ActR-IB, ActR-II(A) and ActR-IIB, have been cloned from mammals. Through alternative mRNA splicing, multiple ActR-IIB isoforms can also be generated, adding to the complexity of the activin receptor system. Different activin isoforms bind with different high-affinities to the various type II isoforms. Type I activin receptors do not bind directly to activin, but will associate with the type II receptor-activin complex and initiate signal transduction. Besides the activin isoforms, ActR-II will also bind inhibin, BMP-2 and BMP-7 with lower affinities. ActR-I can also bind and form signaling complexes with the BMP-2/7-bound BMPR-II. Activin type II receptors are highly conserved. Human, mouse and rat type II activin receptors share greater than 98% amino acid sequence homology. Recombinant soluble activin type II receptors bind activin with high affinity, and are potent activin antagonists.

References
  1. Attisano, L. et al. (1996) Mol. and Cell Biol. 16:1066.
  2. Woodruff, T.K. (1998) Biochem. Pharmacology 55:953.
Long Name
Activin Receptor IIB
Entrez Gene IDs
93 (Human); 11481 (Mouse)
Alternate Names
activin A receptor, type IIB; Activin receptor type IIB; activin receptor type-2B; Activin RIIB; ActivinRIIB; ACTRIIB; ActR-IIB; ACVR2B; EC 2.7.11; EC 2.7.11.30; MGC116908

Citations for Recombinant Human Activin RIIB Fc Chimera (NS0-expressed)

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Structural basis for potency differences between GDF8 and GDF11
    Authors: RG Walker, M Czepnik, EJ Goebel, JC McCoy, A Vujic, M Cho, J Oh, S Aykul, KL Walton, G Schang, DJ Bernard, AP Hinck, CA Harrison, E Martinez-H, AJ Wagers, RT Lee, TB Thompson
    BMC Biol, 2017-03-03;15(1):19.
    Species: Bacteria - Staphylococcus aureus
    Sample Types: Protein
    Applications: Bioassay
  2. Facilitated receptor-recognition and enhanced bioactivity of bone morphogenetic protein-2 on magnesium-substituted hydroxyapatite surface
    Authors: B Huang, Y Yuan, T Li, S Ding, W Zhang, Y Gu, C Liu
    Sci Rep, 2016-04-14;6(0):24323.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Preferential Induction of the T Cell Auxiliary Signaling Molecule B7-H3 on Synovial Monocytes in Rheumatoid Arthritis
    Authors: BR Yoon, YH Chung, SJ Yoo, K Kawara, J Kim, IS Yoo, CG Park, SW Kang, WW Lee
    J. Biol. Chem, 2015-12-23;291(8):4048-57.
    Species: Human
    Sample Types:
    Applications: Bioassay
  4. Rapid Activation of Bone Morphogenic Protein 9 by Receptor-mediated Displacement of Pro-domains
    Authors: Y Kienast, U Jucknischk, S Scheiblich, M Thier, M de Wouters, A Haas, C Lehmann, V Brand, D Bernicke, K Honold, S Lorenz
    J. Biol. Chem, 2015-12-16;291(7):3395-410.
    Species: Human
    Sample Types:
    Applications: Bioassay
  5. A new model for growth factor activation: type II receptors compete with the prodomain for BMP-7.
    Authors: Sengle G, Ono RN, Lyons KM, Bachinger HP, Sakai LY
    J. Mol. Biol., 2008-07-02;381(4):1025-39.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA (detection), Surface Plasmon Resonance

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