Home / Activin RIA/ALK-2 / Recombinant Human Activin RIA/ALK-2 Fc Chimera Protein, CF

Recombinant Human Activin RIA/ALK-2 Fc Chimera Protein, CF

Catalog #: 637-AR
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
637-AR-100
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Citations (4)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Activin RIA/ALK-2 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human BMP-6 (Catalog # 507-BP) is used at 0.25 μg/mL, the concentration of Recombinant Human Activin RIA/ALK-2 Fc Chimera that produces 50% of the optimal binding response is found to be approximately
1-5 μg/mL.
Source
Mouse myeloma cell line, NS0-derived human Activin RIA/ALK-2 protein
Human Activin RIA
(Asp23-Val124 & Phe120-Val124)
Accession # Q53SV1		 IEGRMD Human IgG1
(Pro100-Lys330)		 6-His tag
N-terminus C-terminus
Accession #
Q53SV1
N-terminal Sequence
Analysis
Asp23 & Phe120
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
39 kDa & 28 kDa (monomers)
SDS-PAGE
40-45 kDa & 34-38 kDa, reducing conditions

Product Datasheets

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637-AR

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

637-AR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Activin RIA/ALK-2

Activin RIA, also known as ALK-2, TSK-7L, SKR1, TSR-I, and ACTR-I, is a glycosylated 65 kDa type I receptor in the TGF-beta serine/threonine kinase receptor family (1-3). Binding of TGF-beta superfamily ligands induces formation of a heterotetrameric complex that contains two chains each of a type I and a type II receptor in multiple combinations. The type II receptors phosphorylate the type I receptors which then phosphorylate and activate Smad signal transduction proteins (1, 2). Activin RIA functions in a wide variety of growth and differentiation processes including gastrulation, skeletal system development, and cardiac morphogenesis
(1, 4-7). Mature human Activin RIA consists of a 103 aa extracellular domain (ECD), a 23 aa transmembrane segment, and a 363 aa cytoplasmic region that includes a Gly/Ser-rich GS box and the kinase domain (8, 9). Within the ECD, human Activin RIA shares 99% and 94% aa sequence identity with mouse and rat Activin RIA, respectively. It shares 20%-26% aa sequence identity with other human type I receptors Activin RIB, BMPR-IA, BMPR-IB, and TGF-beta RI. BMP signaling through Activin RIA is enhanced by the direct interaction between Activin RIA and RGM-B/DRAGON, a BMP coreceptor that also associates with other type I and type II receptors (10). Activin RIA can additionally phosphorylate the coreceptor Endoglin and is required for the inhibitory effect of Endoglin on prostate cancer cell motility (11).

References
  1. Wu, M.Y. and C.S. Hill (2009) Dev. Cell 16:329.
  2. Nickel, J. et al. (2009) Cytokine Growth Factor Rev. 20:367.
  3. de Caestecker, M. (2004) Cytokine Growth Factor Rev. 15:1.
  4. Chuva de Sousa Lopes, S.M. et al. (2004) Genes Dev. 18:1838.
  5. Zhang, D. et al. (2003) J. Bone Miner. Res. 18:1593.
  6. van Dinther, M. et al. (2010) J. Bone Miner. Res. 25:1208.
  7. Wang, J. et al. (2005) Dev. Biol. 286:299.
  8. Matsuzaki, K. et al. (1993) J. Biol. Chem. 268:12719.
  9. Attisano, L. et al. (1993) Cell 75:671.
  10. Samad, T.A. et al. (2005) J. Biol. Chem. 280:14122.
  11. Romero, D. et al. (2010) Carcinogenesis 31:359.
Long Name
Activin Receptor IA
Entrez Gene IDs
90 (Human); 11477 (Mouse)
Alternate Names
activin A receptor, type I; Activin RIA; ActivinRIA; ACTRI; ACVR1; ACVR1A; ACVRLK2; ALK2; ALK-2; FOP; SKR1; TSRI

Citations for Recombinant Human Activin RIA/ALK-2 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Facilitated receptor-recognition and enhanced bioactivity of bone morphogenetic protein-2 on magnesium-substituted hydroxyapatite surface
    Authors: B Huang, Y Yuan, T Li, S Ding, W Zhang, Y Gu, C Liu
    Sci Rep, 2016-04-14;6(0):24323.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Soluble Endoglin Specifically Binds Bone Morphogenetic Proteins 9 and 10 via Its Orphan Domain, Inhibits Blood Vessel Formation, and Suppresses Tumor Growth.
    Authors: Castonguay R, Werner ED, Matthews RG, Presman E, Mulivor AW, Solban N, Sako D, Pearsall RS, Underwood KW, Seehra J, Kumar R, Grinberg AV
    J. Biol. Chem., 2011-07-07;286(34):30034-46.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  3. A new model for growth factor activation: type II receptors compete with the prodomain for BMP-7.
    Authors: Sengle G, Ono RN, Lyons KM, Bachinger HP, Sakai LY
    J. Mol. Biol., 2008-07-02;381(4):1025-39.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA (detection), Surface Plasmon Resonance
  4. Regulation of ADMP and BMP2/4/7 at opposite embryonic poles generates a self-regulating morphogenetic field.
    Authors: Reversade B, De Robertis EM
    Cell, 2005-12-16;123(6):1147-60.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Receptor Binding Assay

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