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Recombinant Human Active ERK1 Protein, CF

Catalog #: 1879-KS
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
1879-KS-010
Recombinant Human Active ERK1 Protein SDS-PAGE
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Product Details
Citations (2)
FAQs
Reviews
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Active ERK1 Protein, CF Summary

Product Specifications

Activity
The specific activity of ERK1 was determined to be 42 nmol/min/mg using a myelin basic protein (MBP) substrate and was equivalent to 382 nmol/min/mg as per radiometric assay.

Accession #
NM_002746
N-terminal Sequence
Analysis
Activated by active MEK1 in vitro
Predicted Molecular Mass
44 kDa

Product Datasheets

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1879-KS

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1879-KS

Formulation Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM PMSF and 25% glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: This product is stable at ≤ ‑70°C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.

Scientific Data

SDS-PAGE Recombinant Human Active ERK1 Protein SDS-PAGE View Larger

The approximate molecular weight is 44 kDa and the purity is > 80%.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ERK1

ERK1 is a protein Serine/Threonine kinase that is a member of the extracellular signal-regulated kinases (ERKs) which are activated in response to numerous growth factors and cytokines (1). Activation of ERK1 requires both tyrosine and threonine phosphorylation that is mediated by MEK. ERK1 is ubiquitously distributed in tissues with the highest expression in heart, brain, and spinal cord. Activated ERK1 translocates into the nucleus where it phosphorylates various transcription factors.

References
  1. Boulton, T.G. et al. (1991) Biochemistry 30:278.
Long Name
Extracellular Signal-regulated Kinase 1
Entrez Gene IDs
5595 (Human); 26417 (Mouse); 50689 (Rat)
Alternate Names
EC 2.7.11; ERK1; ERK-1; ERK1p44-MAPK; ERT2; Extracellular signal-regulated kinase 1; extracellular signal-related kinase 1; HS44KDAP; HUMKER1A; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 3; MAP kinase isoform p44; MAPK 1; MAPK 3; MAPK3; MGC20180; Microtubule-associated protein 2 kinase; Mitogen-activated protein kinase 1; mitogen-activated protein kinase 3; P44ERK1; p44-ERK1; p44mapk; PRKM3; PRKM3EC 2.7.11.24

Citations for Recombinant Human Active ERK1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. ERK1/2 inhibits Cullin 3/SPOP-mediated PrLZ ubiquitination and degradation to modulate prostate cancer progression
    Authors: Y Fan, T Hou, W Dan, Y Zhu, B Liu, Y Wei, Z Wang, Y Gao, J Zeng, L Li
    Cell Death and Differentiation, 2022-02-22;0(0):.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  2. Phosphatase-coupled universal kinase assay and kinetics for first-order-rate coupling reaction.
    PLoS ONE, 2011-08-11;6(8):e23172.
    Applications: Enzyme Assay

FAQs

  1. What is the activation protocol for this enzyme?

    • This enzyme is supplied in its activated form, so no activation step is required.

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