Recombinant Human Active Chk1 Protein, CF Summary
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Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1630-KS
| Formulation | Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM Glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | This product is stable at ≤ ‑70°C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles. |
Scientific Data
Reconstitution Calculator
Background: Chk1
Chk1 is a 56 kDa Serine/Threonine protein kinase that was originally identified in fission yeast to play a role in activation of the DNA damage checkpoint in the G2 phase of the cell cycle (1). Chk1 appears to function downstream of several of the known fission yeast checkpoint gene products, including that encoded by Rad3, a gene with sequence similarity to the ATM gene mutated in patients with ataxia telangiectasia (2).
Citations for Recombinant Human Active Chk1 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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PARP1 and CHK1 coordinate PLK1 enzymatic activity during the DNA damage response to promote homologous recombination-mediated repair
Authors: B Peng, R Shi, J Bian, Y Li, P Wang, H Wang, J Liao, WG Zhu, X Xu
Nucleic Acids Research, 2021-07-21;49(13):7554-7570.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay -
Chk1 and 14-3-3 proteins inhibit atypical E2Fs to prevent a permanent cell cycle arrest
Authors: R Yuan, HR Vos, RM van Es, J Chen, BM Burgering, B Westendorp, A de Bruin
EMBO J., 2018-01-23;0(0):.
Species: Human
Sample Types: Protein
Applications: Bioassay -
Checkpoint kinase 1-induced phosphorylation of O-linked ?-N-acetylglucosamine transferase regulates the intermediate filament network during cytokinesis
Authors: Z Li, X Li, S Nai, Q Geng, J Liao, X Xu, J Li
J. Biol. Chem., 2017-10-11;0(0):.
Applications: Bioassay
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