Recombinant Human Active Akt1 Protein, CF Summary
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Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1775-KS
| Formulation | Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 10 mM glutathione, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | This product is stable at ≤ ‑70°C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles. |
Scientific Data
Reconstitution Calculator
Background: Akt1
Akt1, also known as PKB alpha, is a serine/threonine kinase that belongs to the Akt family. Akt1 is activated in cells in response to diverse stimuli such as hormones, growth factors and extracellular matrix components and is involved in glucose metabolism, transcription, survival, cell proliferation, angiogenesis, and cell motility (1). Akt1 is frequently over-expressed and active in many types of human cancers including cancers of colon, breast, brain, pancreas, and prostate as well as lymphomas and leukemias (2).
- Coffer, P.J. et al. (1998) Biochem J. 335:1.2.
- Anderson, K.E. et al. (1998) Curr. Biol. 8:684.
Citations for Recombinant Human Active Akt1 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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MPST deficiency promotes intestinal epithelial cell apoptosis and aggravates inflammatory bowel disease via AKT
Authors: J Zhang, L Cen, X Zhang, C Tang, Y Chen, Y Zhang, M Yu, C Lu, M Li, S Li, B Lin, T Zhang, X Song, C Yu, H Wu, Z Shen
Redox Biology, 2022-09-11;56(0):102469.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation -
UBE2S, a novel substrate of Akt1, associates with Ku70 and regulates DNA repair and glioblastoma multiforme resistance to chemotherapy
Oncogene, 2016-09-05;0(0):.
Species: Human
Sample Types: Protein
Applications: Enzyme Assay -
The PI3K/Akt signal hyperactivates Eya1 via the SUMOylation pathway.
Authors: Sun Y, Kaneko S, Li X, Li X
Oncogene, 2014-06-23;34(19):2527-37.
Species: Human
Sample Types: Cell Lysates
Applications: Enzyme Assay -
Dithiolethione compounds inhibit Akt signaling in human breast and lung cancer cells by increasing PP2A activity.
Authors: Switzer CH, Ridnour LA, Cheng RY, Sparatore A, Del Soldato P, Moody TW, Vitek MP, Roberts DD, Wink DA
Oncogene, 2009-08-24;28(43):3837-46.
Species: Human
Sample Types: Cell Lysates
Applications: Bioassay
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