Recombinant Human 4-1BB Ligand/TNFSF9 Protein
Recombinant Human 4-1BB Ligand/TNFSF9 Protein Summary
Product Specifications
Arg71-Glu254, with an N-terminal Met and a 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
2295-4L
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS, NaCl and PEG with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
2295-4L/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS, NaCl and PEG and with Trehalose. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: 4-1BB Ligand/TNFSF9
4-1BB Ligand (4-1BBL), also known as CD137L, is a type II transmembrane protein that belongs to the TNF superfamily of molecules and plays an important role in immune response activation (1). The 38 kDa human 4-1BB Ligand consists of an 25 aa cytoplasmic domain, a 23 aa transmembrane segment, and a 206 aa extracellular domain (ECD) (2). Within the ECD, human 4-1BB Ligand shares 34% aa sequence identity with mouse and rat 4-1BB Ligand. 4-1BB Ligand is expressed by activated B cells, monocytes, macrophages, dendritic cells (DC), T cells, lymphoma and multiple myeloma cells, hematopoietic stem cells, early myeloid progenitors, neurons, and astrocytes (3-9). A 26 kDa soluble form of 4-1BB Ligand can be released from the surface of activated cells and retains bioactivity (10).
4-1BB Ligand binds to 4-1BB/TNFRSF9/CD137 on activated CD4+ and CD8+ T cells, thymocytes, and NK cells as well as on monocytes, neutrophils, DC, and eosinophils. In response to 4-1BB Ligand binding, 4-1BB transduces a co-stimulatory signal that promotes the proliferation, activation, and survival of CD4+ and CD8+ T cells (4, 11, 12). T cell co-stimulation through CD28 is important for the initial T cell expansion, while 4-1BB acts later in the response (12, 13). 4-1BB Ligand function supports the survival and responsiveness of memory T cells during viral infection (13-15). Reverse signaling through 4-1BB Ligand on monocytes induces the production of inflammatory cytokines (5). On macrophages, 4-1BB Ligand associates in cis with TLR4 and enhances inflammatory cytokine production in response to TLR4 ligation (6). Its expression on early myeloid progenitor cells limits the development of dendritic cells, monocytes, and B cells (9).
- Wang, C. et al. (2009) Immunol. Rev. 229:192.
- Alderson, M.R. et al. (1994) Eur. J. Immunol. 24:2219.
- Reali, C. et al. (2003) J. Neurosci. Res. 74:67.
- DeBenedette, M.A. et al. (1997) J. Immunol. 158:551.
- Langstein, J. et al. (1998) J. Immunol. 160:2488.
- Kang, Y.J. et al. (2007) Nat. Immunol. 8:601.
- Baessler, T. et al. (2010) Blood 115:3058.
- Gullo, C. et al. (2010) PLoS One 5:e10845.
- Lee, S.-W. et al. (2008) Nat. Immunol. 9:917.
- Salih, H.R. et al. (2001) J. Immunol. 167:4059.
- Wen, T. et al. (2002) J. Immunol. 168:4897.
- Cannons, J.L. et al. (2001) J. Immunol. 167:1313.
- Bertram, E.M. et al. (2002) J. Immunol. 168:3777.
- Bukczynski, J. et al. (2004) Proc. Natl. Acad. Sci. USA 101:1291.
- Lin, G.H.Y. et al. (2009) J. Immunol. 182:934.
Citations for Recombinant Human 4-1BB Ligand/TNFSF9 Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Structure of the 4-1BB/4-1BBL complex and distinct binding and functional properties of utomilumab and urelumab
Authors: SM Chin, CR Kimberlin, Z Roe-Zurz, P Zhang, A Xu, S Liao-Chan, D Sen, AR Nager, NS Oakdale, C Brown, F Wang, Y Yang, K Lindquist, YA Yeung, S Salek-Arda, J Chaparro-R
Nat Commun, 2018-11-08;9(1):4679.
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance -
An anti-TL1A antibody for the treatment of asthma and inflammatory bowel disease
Authors: AW Clarke, L Poulton, D Shim, D Mabon, D Butt, M Pollard, V Pande, J Husten, J Lyons, C Tian, AG Doyle
MAbs, 2018-03-05;0(0):1-43.
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance (SPR -
Galectin-9 controls the therapeutic activity of 4-1BB-targeting antibodies.
Authors: Madireddi S, Eun S, Lee S, Nemcovicova I, Mehta A, Zajonc D, Nishi N, Niki T, Hirashima M, Croft M
J Exp Med, 2014-06-23;211(7):1433-48.
Species: Human
Sample Types: Protein
Applications: Bioassay -
Tumor necrosis factor receptor 1 associates with CD137 ligand and mediates its reverse signaling.
Authors: Moh M, Lorenzini P, Gullo C, Schwarz H
FASEB J, 2013-04-25;27(8):2957-66.
Species: Human
Sample Types: Protein
Applications: Bioassay -
Murine insulin growth factor-like (IGFL) and human IGFL1 proteins are induced in inflammatory skin conditions and bind to a novel tumor necrosis factor receptor family member, IGFLR1.
Authors: Lobito AA, Ramani SR, Tom I, Bazan JF, Luis E, Fairbrother WJ, Ouyang W, Gonzalez LC
J. Biol. Chem., 2011-03-31;286(21):18969-81.
Species: Human
Sample Types:
Applications: Surface Plasmon Resonance
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Average Rating: 4 (Based on 2 Reviews)
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Reason for Rating: Useful in the 50ng/mL and below range to form a standard curve.
