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Recombinant His6-SARS Virus Papain-like Protease Protein, CF

Catalog #: E-610
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
E-610-050
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (2)
FAQs
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant His6-SARS Virus Papain-like Protease Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Reaction conditions will need to be optimized for each specific application.  We recommend an initial PLPro concentration of 20-100 nM when using Ubiquitin-AMC (U-550) or Ubiquitin-Rhodamine 110 (U-555) as a subtrate.  
Source
E. coli-derived viral Papain-like Protease protein
Glu1541 - Lys1855 with a N-terminal 6-His tag
Accession #
P0C6U8.1
Predicted Molecular Mass
37 kDa

Product Datasheets

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E-610

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-610

Formulation Supplied as a solution in HEPES, NaCl and TCEP.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Papain-like Protease

The Papain-like protease ("PLPro") from the human SARS coronavirus (Severe Acute Respiratory Syndrome coronavirus) is a cysteine protease located within the non-structural protein 3 (NS3) section of the viral polypeptide.  PLPro activity is required to process the viral polyprotein into functional, mature subunits; specifically, PLPro cleaves a site at the amino-terminus of the viral replicase region.  In addition to its role in viral protein maturation, PLPro possesses a deubiquitinating and deISGylating activity.  In vivo, this protease antagonizes innate immunity by inhibiting IRF3-induced production of type I interferons.  PLPro has been reported to hydrolyze both K48- and K63 linked poly-Ubiquitin chains in vitro.  When used at low concentrations, the enzyme demonstrates a strong preference for K48-linked tetra-Ubiquitin chains which are primarily converted to di-Ubiquitin species.  

References
  1. Frieman M., et al.  (2009) J.Virol.  83: 6689.
  2. Lindner H.A.,et al.  (2007) Archv. Biochem. Biophys.  466: 8.
  3. Ratia K., et al. (2014) PLoS Pathog. 10:e1004113.
  4. Russell N., et al. (2016) FASEB Ubiquitin & Cell Reg.  (poster)
 
Alternate Names
Papain-like Protease; PLpro; PL-PRO; pp1a; Replicase polyprotein 1a

Citations for Recombinant His6-SARS Virus Papain-like Protease Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. SARS-CoV-2 papain-like protease activates nociceptors to drive sneeze and pain
    Authors: Mali, SS;Silva, R;Gong, Z;Cronce, M;Vo, U;Vuong, C;Moayedi, Y;Cox, JS;Bautista, DM;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Translesion polymerase kappa-dependent DNA synthesis underlies replication fork recovery
    Authors: P Tonzi, Y Yin, CWT Lee, E Rothenberg, TT Huang
    Elife, 2018-11-13;7(0):.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay

FAQs

  1. Is Recombinant Human ISG15 Fluorescein Protein, CF (Catalog # UL-613) a suitable substrate for Recombinant His6-SARS Virus Papain-like Protease Protein, CF (Catalog # E-610)?

    • Recombinant His6-SARS Virus Papain-like Protease Protein, CF (Catalog # E-610) only cleaves at the C-terminus of ISG15 and/or Ubiquitin conjugates. Recombinant Human ISG15 Fluorescein Protein, CF (Catalog # UL-613) is not a suitable substrate because the fluorescein-labeled lysine residues are distributed throughout the protein, but not at the C-terminus. Suitable substrate candidates for catalog # E-610 include include Recombinant Human Ubiquitin AMC Protein, CF (Catalog #U-550), Recombinant Human Ubiquitin Rhodamine 110 Protein, CF (Catalog # U-555), and Recombinant Human ISG15 AMC Protein, CF (Catalog # UL-553).

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