Recombinant F. meningosepticum Endo F1 Protein, CF
Recombinant F. meningosepticum Endo F1 Protein, CF Summary
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Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
5220-GH
Formulation | Supplied as a 0.2 μm filtered solution in Tris and NaCl. |
Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date. |
Reconstitution Calculator
Background: Endo-beta-N-acetylglucosaminidase F1/Endo F1
N-glycans are commonly found on various glycoproteins. While peptide N-glycosidase from Flavobacterium meningosepticum (PNGase F) is widely used to release virtually all types of N-glycans under denaturing conditions, Endo-beta -N-acetylglucosaminidases from the same bacterial species, including Endo F1, can be used under native conditions to specifically release particular types of N-glycans (1, 2). Because these glycosidases hydrolyze the chitobiose core ofN-glycans, the released glycan products will contain one GlcNAc residue at their reducing ends with the other GlcNAc residue remaining attached to an asparagine residue on the glycoprotein. Endo F1 specifically releases oligomannose and hybrid but not complex type N-glycans from glycoproteins (3, 4). This enzyme is also suitable to deglycosylate substrates under denaturing conditions and remains active on sulfated and core fucosylated N-glycans at reduced rates (4).
- Maley, F. et al. (1989) Anal. Biochem. 180:195.
- Tarentino, A.L. et al. (1985) Biochemistry. 24:4665.
- Tarentino, A.L. et al. (1992) J. Biol. Chem. 267:3868.
- Trimble, R.B. and Tarentino, A.L. (1991) J. Biol. Chem. 266:1646.
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